Sains Malaysiana 47(12)(2018): 3051–3059

http://dx.doi.org/10.17576/jsm-2018-4712-15

 

Sesquiterpene Production in Methyl Jasmonate-Induced Persicaria minor Cell Suspension Culture

(Penghasilan Seskuiterpena dalam Kultur Ampaian Sel Persicaria minor Diinduksi Metil Jasmonat)

 

PIRIYATASNI SELLAPAN, EMELDA ROSSELEENA ROHANI & NORMAH MOHD NOOR*

 

Institute of Systems Biology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

 

Diserahkan: 30 Mei 2018/Diterima: 18 September 2018

 

ABSTRACT

Sesquiterpenes are a three-isoprene unit compounds which belong to terpenoid family of secondary metabolites. These volatile compounds are one of the major constituents of essential oils in plants and plays major roles in plant signaling of defense mechanism. The effects of methyl jasmonate (MeJA) concentrations (100 and 200 μM) on the production of sesquiterpene compounds after incubation period for 1, 3, and 6 days were investigated in Persicaria minor cell suspension culture. Headspace Solid-Phase Microextraction (HS-SPME) method was used to absorb volatile compounds from suspension cells and liquid medium. They were then analyzed through Gas Chromatography-Mass Spectrometry (GC-MS) to identify sesquiterpene compounds. Among the 15 sesquiterpene compounds identified, α- muurolene was found in significantly higher concentration in all MeJA treated cultures. The results showed that α-muurolene was detected in the suspension cells at the highest peak area of 14.17% at 100 μM MeJA treated cultures with 3-day incubation. Analysis of liquid medium of the treatments identified secretion of α-muurolene into the culture medium, with total peak area of 0.72%. These results showed that sesquiterpene production in MeJA induced P. minor suspension culture depended on the MeJA concentration and also culture incubation period.

 

Keywords: α-muurolene; cell suspension culture; GC-MS; methyl jasmonate; sesquiterpene

 

ABSTRAK

Seskuiterpena adalah unit sebatian tiga isoprena yang tergolong dalam keluarga terpenoid metabolit sekunder. Sebatian meruap ini adalah juzuk utama dalam minyak pati daripada tumbuhan dan memainkan peranan penting sebagai pengisyaratan bagi mekanisme pertahanan tumbuhan. Kesan kepekatan metil jasmonat (MeJA) (100 dan 200 μM) ke atas penghasilan seskuiterpena dengan tempoh masa pengeraman 1, 3 dan 6 hari telah dikaji dalam kultur ampaian sel Persicaria minor. Pengekstrakan-mikro ruang kepala fasa (Headspace Solid-Phase Microextraction) (HS-SPME) bersama kromatografi gas-spektrometri jisim (GC-MS) digunakan untuk mengenal pasti sebatian seskuiterpena daripada ekstrak ampaian sel. Daripada 15 sebatian seskuiterpena yang dikenal pasti, α-muurolin didapati hadir pada kepekatan yang signifikan bagi kesemua kultur yang diberi perlakuan MeJA. Keputusan kajian menunjukkan α-muurolin dikesan pada luas puncak tertinggi 14.71% dalam ampaian sel pada 100 μM MeJA dengan tiga hari pengeraman. Analisis ke atas medium cecair mendapati kehadiran rembesan α-muurolin dengan luas puncak keseluruhan 0.72%. Keputusan menunjukkan bahawa penghasilan seskuiterpena dalam ampaian sel terinduksi MeJA bagi P. minor adalah bergantung kepada kepekatan MeJA dan juga tempoh pengeraman.

 

Kata kunci: α-muurolin; GC-MS; kultur ampaian sel; metil jasmonat; seskuiterpena

RUJUKAN

Ahmad, R., Baharum, S.N., Bunawan, H., Lee, M., Noor, N.M., Rohani, E.R., Ilias, N. & Zin N.M. 2014. Volatile profiling of aromatic medicinal plant, Polygonum minus in different tissues and its biological activities. Molecules 19(11): 19220- 19242.

Antunes, E., Gordo, W.M., de Oliveira, J.F., Teixeira, C.E., Hyslop, S. & De Nucci, G. 2001. The relaxation of isolated rabbit Corpus cavernosum by the herbal medicine Catuama and its constituents. Phytotherapy Research 15(5): 416-421.

Ashraf, M.F., Che Mohd Zain, C.R., Zainal, Z., Ismail, I., Noor, N.M., Anuar, N., Markom, M. & Ismail, I. 2015. Establishment of Persicaria minor hairy roots and analysis of secreted β-caryophyllene in medium broth. Plant Cell, Tissue and Organ Culture 121(1): 11-20.

Baharum, S.N., Bunawan, H., Ghani, M.A., Mustapha, W.A.W. & Noor, N.M. 2010. Analysis of the chemical composition of the essential oil of Polygonum minus Huds. using two-dimensional gas chromatography-time-of-flight mass spectrometry (GC-TOF MS). Molecules 15: 7006-7015.

Bona, C.M., Santos, G.D. & Biasi, L.A. 2012. Lavandula calli induction, growth curve and cell suspension formation. Revista Brasileira de Ciências Agrárias 7(1): 17-23.

Cai, Z., Kastell, A., Knorr, D. & Smetanska, I. 2012. Exudation: An expanding technique for continuous production and release of secondary metabolites from plant cell suspension and hairy root cultures. Plant Cell Reports 31(3): 461-477.

Cai, Z., Kastell, A., Mewis, I., Knorr, D. & Smetanska, I. 2011. Polysaccharide elicitors enhance anthocyanin and phenolic acid accumulation in cell suspension cultures of Vitis vinifera. Plant Cell, Tissue and Organ Culture 108(3): 401-409.

Da Silva, A.L., Piato, A.L.S., Bardini, S., Netto, C.A., Nunes, D.S. & Elisabetsky, E. 2004. Memory retrieval improvement by Ptychopetalum olacoides in young and aging mice. Journal of Ethnopharmacology 95(2-3): 199-203.

Dörnenburg, H. & Knorr, D. 1995. Strategies for the improvement of secondary metabolome production in plant cell cultures. Enzyme and Microbial Technology 17: 674-684.

Dursun, A., Güler, Z., Özkan, D. & Bozdoğan-Konuşkan, D. 2017. Identification of volatile compounds (VCs) in the leaves collected from 'Gemlik', 'Halhali' and 'Sari Hasebi' olive tree varieties. International Journal of Secondary Metabolite 4(3): 195-204.

Ee, S.F., Mohamed-Hussein, Z.A., Othman, R., Shaharuddin, N.A., Ismail, I. & Zainal, Z. 2014. Functional characterization of sesquiterpene synthase from Polygonum minus. The Scientific World Journal 2014: 840592.

Gao, F., Yong, Y. & Dai, C. 2011. Effects of endophytic fungal elicitor on two kinds of terpenoids production and physiological indexes in Euphorbia pekinensis suspension cells. Journal of Medicinal Plants Research 5(18): 4418- 4425.

Ismail, I., Gor, M.C., Mohammed-Hussein, Z.A. & Noor, M.N. 2011. Alteration of abiotic stress responsive genes in Polygonum minus roots by jasmonic acid elicitation. In Plants and Environment, edited by Vasanthaiah Kambiranda, D. Rijeka, Croatia: InTech-Open Access Company. pp. 49-88.

Ito, M., Okimoto, K., Yagura, T., Honda, G., Kiuchi, F. & Shimada, Y. 2005. Induction of sesquiterpenoid production by methyl jasmonate in Aquilaria sinensis cell suspension culture. Journal of Essential Oil Research 17(2): 175-180.

Kang, S.M., Min, J.Y. & Kim, Y.D. 2006. Effects of methyl jasmonate and salicylic acid on the production of bilobalide and ginkgolides in cell cultures of Ginkgo biloba. In Vitro Cellular & Developmental Biology-Plant 42: 44-49.

Kang, S.M., Min, J.Y., Kim, Y.D., Karigar, C.S., Kim, S.W. & Goo, G.H. 2009. Effect of biotic elicitors on the accumulation of bilobalide and ginkgolides in Ginkgo biloba cell cultures. Journal of Biotechnology 139(1): 84-88.

Keng, C.L., Wei, A.S. & Bhatt, A. 2010. Elicitation effect on cell biomass and production of alkaloids in cell suspension culture of the tropical tree Eurycoma longifolia. Research Journal of the Costa Rican Distance Education University 2(2): 239-244.

Kumeta, Y. & Ito, M. 2010. Characterization of δ-guaiene synthases from cultured cells of Aquilaria, responsible for the formation of the sesquiterpenes in Agarwood. Plant Physiology 154(4): 1998-2007.

Liew, P.S. & Hair-Bejo, M. 2015. Farming of plant-based veterinary vaccines and their applications for disease prevention in animals. Advances in Virology 209434: 1-12.

Mohamad-Zuldin, N.N., Said, I.M., Noor, N.M., Zainal, Z., Chew, J.K. & Ismail, I. 2013. Induction and analysis of the alkaloid mitragynine content of a Mitragyna speciosa suspension culture system upon elicitation and precursor feeding. The Scientific World Journal 209434: 1-11.

Murashige, T. & Skoog, F. 1962. A revised medium for rapid growth and bioassay with tabacco cultures. Physiology of Plant 15: 473-497.

Ochoa-Villarreal, M., Howat, S., Hong, S., Jang, M.O., Jin, Y.W., Lee, E.K., & Loake, G.J. 2016. Plant cell culture strategies for the production of natural products. BMB Reports 49(3): 149-158.

Pépin, M., Archambault, J., Chavarie, C. & Cormier, F. 2004. Growth kinetics of Vitis vinifera cell suspension cultures: I. Shake flask cultures. Biotechnology and Bioengineering 47(2): 131-138.

Qader, S.W., Abdulla, M.A., Chua, L.S. & Hamdan, S. 2012. Potential bioactive property of Polygonum minus Huds (kesum) review. Scientific Research and Essays 7(2): 90-93.

Sakhya, P., Marslin, G., Siram, K., Beerhues, L. & Franklin, G. 2017. Elicitation as a tool to improve the profiles of high-value secondary metabolites and pharmacological properties of Hypericum perforatum. Journal of Pharmacy and Pharmacology 71(1): 70-82.

Shaaltiel, Y., Bartfeld, D., Hashmueli, S., Baum, G., Brill-Almon, E., Galili, G., Dym, O., Boldin-Adamsky, S.A., Silman, I., Sussman, J.L., Futerman, A.H. & Aviezer, D. 2007. Production of glucocerebrosidase with terminal mannose glycans for enzyme replacement theraphy of Gaucher's disease using a plant cell system. Plant Biotechnology Journal 5(5): 579-590.

Shukor, M.F.A., Ismail, I., Zainal, Z. & Noor, N.M. 2013. Development of a Polygonum minus cell suspension culture system and analysis of secondary metabolites enhanced by elicitation. Acta Physiology Plant 35(5): 1675-1689.

Siqueira, I.R., Fochesatto, C., da Silva, A.L., Nunes, D.S., Battastini, A.M., Netto, C.A. & Elisabetsky, E. 2003. Ptychopetalum olacoides, a traditional Amazonian 'nerve tonic', possesses anticholinesterase activity. Pharmacology Biochemistry and Behavior 75(3): 645-650.

Smetanska, I. 2008. Production of secondary metabolites using plant cell cultures. Food Biotechnology 111: 187-228.

Srivastava, P., Sisodia, V. & Chaturvedi, R. 2011. Effect of culture conditions on synthesis of triterpenoids in suspension cultures of Lantana camara L. Bioprocess and Biosystems Engineering 34(1): 75-80.

Vaz, Z.R., Mata, L.V. & Calixto, J.B. 1998. Analgesic effect of the herbal medicine Catuama in thermal and chemical models of nociception in mice. Phytotherapy Research 11(2): 101-106.

Vikram, P., Chiruvella, K.K., Ripain, I.H.A. & Arifullah, M. 2014. A recent review on phytochemical constituents and medicinal properties of kesum (Polygonum minus Huds.). Asian Pacific Journal of Tropical Biomedicine 4(6): 430-435.

Yaacob, K.B. 1987. Kesom oil: A natural source of aliphatic aldehydes. Perfumer Flavorist 12: 27-30.

 

*Pengarang untuk surat-menyurat; email: profnormah@yahoo.com

 

 

 

 

 

 

sebelumnya