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Sains Malaysiana 47(6)(2018): 1311–1318
http://dx.doi.org/10.17576/jsm-2018-4706-28
Immobilization of Bovine Serum Albumin on
the Chitosan/PVA Film
(Imobilisasi Serum Bovin pada Filem Kitosan/PVA)
NURUL MUJAHIDAH
AHMAD
KHAIRUDDIN1*,
AMALINA
MUHAMMAD
AFIFI1,4,
NUR
AWANIS
HASHIM2,
SHAZA EVA
MOHAMAD3
& KATAYOON KALANTARI1,4
1Department of Mechanical Engineering, University
of Malaya, 50603 Kuala Lumpur,
2Department of Chemical Engineering, University of Malaya, 50603 Kuala Lumpur, Wilayah Persekutuan,
Malaysia
3Department of Environmental and Green Technology (EGT), Malaysia
Japan International Institute of Technology (MJIIT), Universiti
Teknologi Malaysia, Jalan Sultan Yahya Petra, 54100 Kuala Lumpur, Malaysia
4Centre of Advanced Materials (CAM), Faculty of Engineering,
University of Malaya, 50603 Kuala Lumpur, Wilayah Persekutuan,
Malaysia
Diserahkan: 21 Ogos 2017/Diterima: 26 Januari
2018
ABSTRACT
Chitosan/polyvinyl alcohol (Chitosan/PVA) blended film was prepared by direct blend process and
solution casting methods. In order to reduce the swelling ratio
and enhance the chemical and mechanical stability, Chitosan/PVA film
was cross-linked with glutaraldehyde in order to produce Chitosan-g-PVA.
Bovine serum albumin (BSA) was used as a model protein to incorporate
into the Chitosan-g-PVA. The chemical structure and
morphological characteristics of films were studied by FT-IR and
scanning electron microscopy (SEM). Mechanical and physical
properties of blended films such as tensile properties in the dry
and wet states, water uptake and water contact angle measurement
were characterized. Blending PVA and chitosan improved strength and flexibility of the
films. Crosslinking with glutaraldehyde further improves the tensile
strength and decrease the hydrophilicity of films. BSA immobilized on the Chitosan-g-PVA film was calculated as BSA encapsulation
efficiency.
Keywords: Bovine serum albumin;
chitosan; glutaraldehyde; polyvinyl alcohol
ABSTRAK
Filem Kitosan/polivinil alkohol (Kitosan/PVA) telah dihasilkan melalui
proses pengadunan dan teknik pembentukan larutan. Dalam
usaha untuk mengurangkan nisbah pembengkakan dan meningkatkan kestabilan
mekanikal dan kimia, Kitosan/PVA filem
telah ditaut silang dengan menggunakan glutaraldehid untuk menghasilkan
Kitosan-g-PVA. Serum bovin albumin (SBA)
telah digunakan sebagai model protein untuk dicampurkan ke dalam
Kitosan-g-PVA.
Struktur kimia dan ciri morfologi filem telah
dikaji dengan menggunakan FT-IR dan mikroskop elektron pengimbas
(SEM).
Sifat mekanikal dan fizikal filem seperti sifat tegangan filem dalam
keadaan kering dan basah, kadar pengambilan
air dan pengukuran sudut sentuhan air dicirikan. Pengadunan
PVA
dan kitosan memperbaiki kekuatan dan keluwesan filem.
Proses taut silang dengan glutaraldehid meningkatkan lagi kekuatan
tegangan dan mengurangkan sifat hidrofili filem. Immobilisasi
SBA pada
filem Kitosan-g-PVA dinilai sebagai kecekapan pengkapsulan
SBA.
Kata
kunci: Glutaraldehid; kitosan; polivinil alkohol; serum bovin albumin
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*Pengarang untuk surat-menyurat; email: nurul2209@gmail.com
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