Sains Malaysiana 47(9)(2018): 1999–2008

http://dx.doi.org/10.17576/jsm-2018-4709-07

 

Optimisation of Cinnamaldehyde-in-water Nanoemulsion Formulation using Central Composite Rotatable Design

(Pengoptimuman Formulasi Nanoemulsi Sinamaldehid dalam Air Menggunakan Reka Bentuk Komposit Putaran Tengah)

 

ASMAWATI M. SAIL1,2, WAN AIDA WAN MUSTAPHA1*, SALMA MOHAMAD YUSOP1,

MOHAMAD YUSOF MASKAT1 & AHMAD FUAD SHAMSUDDIN3,4

 

1School of Chemical Science and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

 

2Agricultural Product Technology Department, Faculty of Agriculture, Syiah Kuala University, Banda Aceh 23111, Indonesia

 

3Centre for Drug Delivery Research, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, 50300 Kuala Lumpur, Wilayah Persekutuan, Malaysia

4Faculty of Pharmacy and Health Science, Universiti Kuala Lumpur Royal College of Medicine Perak, No 3 Jalan Greentown, 30450 Ipoh, Perak Darul Ridzuan, Malaysia

 

Diserahkan: 12 September 2017/Diterima: 1 Jun 2018

 

 

ABSTRACT

Thirteen formulations of cinnamaldehyde/non-ionic surfactant/water system nanoemulsions were prepared using high-pressure homogenisation. The result showed that varying the cinnamaldehyde/surfactant ratio had effect significantly (p<0.05) to mean droplet diameter, polidispersity index, ζ-potential, turbidity and whiteness index, while no significant effect (p>0.05) to viscosity. The mean droplet diameter ranged from 50.48 to 106.4 nm, polydispersity index from 0.06 to 0.28 and ζ-potential from -4.11 to -6.98 mV. The smallest droplet size was produced using 5% cinnamaldehyde and 5% Tween 80. Response surface for droplet diameter showed that the higher the cinnamaldehyde and surfactant concentrations, the larger the droplet diameter, polydispersity index and whiteness index. However, the ζ-potential increased as the cinnamaldehyde concentration decreased and Tween 80 increased. Increasing the cinnamaldehyde concentration led to an increase in turbidity. Formulation of 5% cinnamaldehyde and 6.23% Tween 80 gave no observable separation of the nanoemulsion with minimum droplet size, polidispersity index, viscosity, turbidity, whiteness index and maximum ζ-potential in modulus. The stability of the optimum formulation was sustained for 10 days upon storage at 4°C. The values of droplet diameter, PDI and ζ-potential were 55.50 nm, 0.08 and -5.38 mV, respectively.

 

Keywords: Cinnamaldehyde; formation; high-pressure homogenisation; nanoemulsion; optimization

 

ABSTRAK

Tiga belas formulasi nanoemulsi sistem sinamaldehid/surfaktan bukan ion/air telah disediakan menggunakan homogenisasi tekanan tinggi. Keputusan menunjukkan bahawa perlakuan nisbah sinamaldehid/surfaktan memberi kesan yang signifikan (p<0.05) terhadap rerata diameter titisan, indeks polidispersiti, ζ-potensial, kekeruhan dan indeks keputihan, namun tidak berpengaruh signifikan (p>0.05) terhadap kepekatan. Nilai rerata diameter titisan berkisar antara 50.48 hingga 106.4 nm, indeks polidispersiti daripada 0.06 hingga 0.28, dan ζ-potensial dari -4.11 hingga -6.98 mV. Saiz titisan terkecil dihasilkan menggunakan sinamaldehid 5% dan 5% Tween 80. Tindak balas permukaan untuk diameter titisan menunjukkan bahawa lebih tinggi kepekatan sinamaldehid dan surfaktan, semakin besar pula diameter titisan, indeks polidispersiti dan indeks keputihan. Walau bagaimanapun, potensi ζ meningkat apabila kepekatan sinamaldehid menurun dan Tween 80 meningkat. Peningkatan kepekatan sinamaldehid menyebabkan peningkatan kekeruhan. Formulasi sinamaldehid 5% dan 6.23% Tween 80 tidak ditemukan adanya pengasingan nanoemulsi dengan diameter saiz titisan, indeks polidispersiti, kelikatan, kekeruhan dan indeks keputihan yang minimum dan maksimum nilai ζ-potensial dalam modulus. Kestabilan formulasi optimum dikekalkan selama 10 hari tempoh penyimpanan pada suhu 4°C. Nilai diameter titisan, indeks polidispersiti dan ζ-potensial masing-masing adalah 55.50 nm, 0.08 dan -5.38 mV.

 

Kata kunci: Homogenisasi tekanan tinggi; nanoemulsi; pembentukan; pengoptimuman; sinamaldehid

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*Pengarang untuk surat-menyurat; email: wanaidawm@ukm.edu.my

 

 

 

 

 

 

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