Sains Malaysiana 50(10)(2021): 2859-2867

http://doi.org/10.17576/jsm-2021-5010-01

Effect of Larval Food Amount on in vitro Rearing of Indo-Malayan Stingless Bee Queen, Heterotrigona itama (Hymenoptera: Apidae; Meliponini)

(Kesan Jumlah Makanan Larva Ternakan in vitro Ratu Kelulut Indo-Malaya, Heterotrigona itama (Hymenoptera: Apidae; Meliponini))

NURUL IZDIHAR RAZALI¹, SHAMSUL BAHRI ABD RAZAK², FATIMAH HASHIM¹, NURUL WAHIDA OTHMAN³ & WAHIZATUL AFZAN AZMI^1,4*

¹Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu Darul Iman, Malaysia

²Faculty of Fishery and Food Science, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu Darul Iman, Malaysia

³Department of Biological Sciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

⁴Institute of Tropical Biodiversity and Sustainability Development, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu Darul Iman, Malaysia

Diserahkan: 17 Ogos 2020/Diterima: 22 Februari 2021

ABSTRACT

The demand for stingless bee colonies in Malaysia has considerably increased due to the rapid advance of meliponiculture in using the stingless bees as agricultural pollinators, as well as the commercialization of stingless bee products (i.e. honey, bee bread and propolis). Thus, in vitro queen rearing for a large scale and rapid colony multiplication must be developed in order to fulfil the public requirements in a short period. Little is known about the in vitro rearing of native stingless bee queen, Heterotrigona itama. Therefore, in this study, we investigated the amount of larval food required by H. itama queen by comparing three different amounts of larval, viz., 100 µL, 120 µL and 150 µL. All treatments were controlled under 100% relative humidity for the first 6 days, and 75% relative humidity for the rest of larval development until queen adult emergence, under 30 °C incubator temperature. The results showed that larvae of H. itama treated with the highest amount of larval food (150 µL) led to 78% of the queen’s emergence, whereas larvae treated with 120 µL and 100 µL of larval resulted in 40% and 0% of queen emergence. The dynamic survival curve showed that most of the larvae died before the pupation phase and reached constant stability afterward. The queen’s body and abdominal length were significantly greater than wild workers. Microscopy analysis showed that in vitro queen had well-developed reproductive system with a huge ovary and spermatheca, whereas wild worker had much smaller ovary without spermatheca. Outcomes from this study could help increase the number of colonies on a large scale, allowing for their use both ecologically and economically, and contribute to conservation efforts in native species of stingless bees.

Keywords: Heterotrigona itama; Indo-Malayan stingless bee; in vitro rearing; larval food; queen

ABSTRAK

Permintaan untuk koloni kelulut di Malaysia meningkat dengan ketara disebabkan oleh perkembangan pesat meliponikultur yang menggunakan kelulut sebagai pendebunga pertanian dan pengkomersilan produk kelulut (i.e. madu, debunga lebah, propolis). Oleh itu, pembiakbakaan ratuin vitro dalam skala besar dan penggandaan koloni secara cepat perlu dilaksanakan untuk memenuhi permintaan awam dalam jangka masa yang pendek. Walau bagaimanapun, pengetahuan pembiakbakaan ratu kelulutHeterotrigona itama secarain vitro, masih tidak banyak diketahui. Oleh itu, dalam kajian ini, kami mengkaji jumlah makanan larva yang diperlukan oleh larva ratu kelulutH. itama dengan membandingkan tiga jenis jumlah makanan larva iaitu 100 µL, 120 µL dan 150 µL. Ketiga-tiga rawatan dikawal di bawah kelembapan relatif 100 % untuk 6 hari pertama dan 75 % kelembapan relatif bagi keseluruhan pembesaran larva sehingga menjadi ratu dewasa dengan suhu 30°C di dalam inkubator. Keputusan menunjukkan larva H. itama yang diberi dengan kuantiti makanan yang tertinggi (150 µL) menyumbang kepada 78% penghasilan ratu, manakala larva yang dirawat dengan 120 µL dan 100 µL makanan larva menghasilkan 40% dan 0% ratu. Cerun kelangsungan hidup menunjukkan kebanyakan larva mati sebelum fasa pupa dan seterusnya mampu mencapai kestabilan malar. Badan dan panjang abdomen ratu adalah lebih besar secara signifikan berbanding pekerja liar. Analisis mikroskopi ratuin vitro menunjukkan ratu mempunyai sistem pembiakan yang lengkap dengan ovari yang besar berserta spermateka, manakala pekerja liar mempunyai ovari yang lebih kecil tanpa spermateka. Hasil daripada kajian ini dapat membantu untuk meningkatkan bilangan koloni berskala besar, supaya ia boleh dimanfaatkan untuk keperluan ekologi dan ekonomi, serta menyumbang kepada usaha pemeliharaan spesies kelulut asli.

Kata kunci: Heterotrigona itama; kelulut Indo-Malaya; makanan larva; pembelaanin vitro; ratu

RUJUKAN

Azmi, W.A., Wan Sembok, W.Z., Yusuf, N., Mohd. Hatta, M.F., Salleh, A.F., Hamzah, M.A.H., & Ramli, S.N. 2019. Effects of pollination by the Indo-Malaya stingless bee (Hymenoptera: Apidae) on the quality of greenhouse-produced rockmelon. Journal of Economic Entomology 112(1): 20-24.

Azmi, W.A., Samsuri, N., Hatta, M.F.M., Ghazi, R. & Seng, C.T. 2017. Effects of stingless bee (Heterotrigona itama) pollination on greenhouse cucumber (Cucumis sativus). Malaysian Applied Biology 46(1): 51-55.

Baptistella, A.R., Souza, C.C., Santana, W.C. & Soares, A.E.E. 2012. Techniques for the in vitro production of queens in stingless bees (Apidae, Meliponini). Sociobiology 59(1): 297-310.

Camargo, C.D. 1972. Determinação de castas em Scaptotrigona postica Latreille (Hymenoptera, Apidae). Revista Brasileira de Biologia. 32(1): 133-138.

Contrera, F.A.L., Menezes, C. & Venturieri, G.C. 2011. New horizons on stingless beekeeping (Apidae, Meliponini). Revista Brasileira de Zootecnia 40: 48-51.

Cruz-Landim, C.D. 2000. Ovarian development in Meliponine bees (Hymenoptera: Apidae): The effect of queen presence and food on worker ovary development and egg production. Genetics and Molecular Biology 23(1): 83-88.

Department of Agriculture. 2017. Statistics of Stingless Beekeepers in Malaysia. Putrajaya: Ministry of Agriculture of Malaysia.

dos Santos, C.F., dos Santos, P.D.D.S. & Blochtein, B. 2016. In vitro rearing of stingless bee queens and their acceptance rate into colonies. Apidologie 47(4): 539-547.

Engels, W. & Imperatriz-Fonseca, V.L. 1990. Caste development, reproductive strategies and control of fertility in honey bees and stingless bees. In Social Insects, edited by Engels, W. Berlin, Germany: Springer-Verlag. pp. 167-230.

Faustino, C.D., Silva-Matos, E.V., Mateus, S. & Zucchi, R. 2002. First record of emergency queen rearing in stingless bees (Hymenoptera, Apinae, Meliponini). Insectes Sociaux 49(2): 111-113.

Hartfelder, K., Makert, G.R., Judice, C.C., Pereira, G.A., Santana, W.C., Dallacqua, R. & Bitondi, M.M. 2006. Physiological and genetic mechanisms underlying caste development, reproduction and division of labor in stingless bees. Apidologie 37(2): 144-163.

Imperatriz-Fonseca, V.L. & Zucchi, R. 1995. Virgin queens in stingless bee (Apidae, Meliponinae) colonies: A review. Apidologie 26(3): 231-244.

Ismail, M.M. & Ismail, W.I.W. 2018. Development of stingless beekeeping projects in Malaysia. E3S Web of Conferences 52: 00028.

Kokich, V.G. 2013. In-vitro vs in-vivo materials research. American Journal of Orthodontics and Dentofacial Orthopedics 143(4): S11.

Kwapong, P., Aidoo, K., Combey, R. & Karikari, A. 2010. Stingless Bees - Importance, Management and Utilization: A Training Manual for Stingless Beekeeping. Accra, Ghana: Unimax Macmillan Ltd. p. 72.

Maciel-Silva, V.L. & Kerr, W.E. 1991. Sex determination in bees. XXVII. Castes obtained from larvae fed homogenized food in Melipona compressipes (Hymenoptera, Apidae). Apidologie 22(1): 15-19.

Menezes, C., Vollet-Neto, A. & Fonseca, V.L.I. 2013. An advance in the in vitro rearing of stingless bee queens. Apidologie 44(5): 491-500.

Michener, C.D. 2000. The Bees of the World. London: The Johns Hopkins University Press. p. 913.

Mohd Norowi, H., Sajap, A.S., Rosliza, J., Mohd Fahimie, J. & Suri, R. 2008. Conservation and sustainable utilization of stingless bees for pollination services in agricultural ecosystems in Malaysia. Putrajaya: Department of Agriculture. p. 12.

Rasmussen, C. 2008. Catalog of the Indo-Malayan/Australasian stingless bees (Hymenoptera: Apidae: Meliponini). Auckland: Magnolia Press. pp. 15-17.

Sakagami, S.F. 1982. Stingless bees. In Social Insects. Vol. III, edited by Hermann, H. New York: Academic Press. pp. 361-423.

Samsudin, S.F., Mamat, M.R. & Hazmi, I.R. 2018. Taxonomic study on selected species of stingless bee (Hymenoptera: Apidae: Meliponini) in Peninsular Malaysia. Serangga 23(2): 2013-258.

Slaa, E.J., Chaves, L.A.S., Malagodi-Braga, K.S. & Hofstede, F.E. 2006. Stingless bees in applied pollination: Practice and perspectives. Apidologie 37(2): 293-315.

Solomon, M.E. 1951. Control of humidity with potassium hydroxide, sulphuric acid, or other solutions. Bulletin of Entomological Research 42(3): 543-554.

Wheeler, D.E. 1986. Developmental and physiological determinants of caste in social Hymenoptera: Evolutionary implications. The American Naturalist 128(1): 13-34.

Winston, P.W. & Bates, D.H. 1960. Saturated solutions for the control of humidity in biological research. Ecology 41(1): 232-237.

*Pengarang untuk surat-menyurat; email: wahizatul@umt.edu.my