Sains Malaysiana 50(12)(2021): 3557-3567

http://doi.org/10.17576/jsm-2021-5012-08  

Pengenalpastian Bakteria Tanah yang Mempunyai Aktiviti Antikulat terhadap Patogen Kelapa Sawit, Ganoderma boninense

(Identification of Soil Bacteria with Antifungus Activity towards Palm Oil Pathogen, Ganoderma boninense)  

AQILAH YUSOFF1, FATIN HUMAIRAH M. ASHAARI1, MUHAMMAD ASYRAFF ABD SAMAD1,

ANIS FARHAN FATIMI AB WAHAB1,2 & IZWAN BHARUDIN1,3*

 

1Department of Biological Sciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

 

2FGV Innovation Centre (Biotechnology), Pt. 23417 Lengkuk Teknologi, 71760 Bandar Enstek, Negeri Sembilan Darul Khusus, Malaysia

 

3Pusat Penyelidikan Bukit Fraser (PPBF), Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

 

Diserahkan: 29 Mac 2020/Diterima: 16 April 2021

 

ABSTRAK

Penyakit reput pangkal batang (BSR) yang berpunca daripada kulat fitopatogen Ganoderma boninense merupakan ancaman terbesar kepada tanaman kelapa sawit di Malaysia. BSR menyebabkan pengurangan hasil tandan sawit dan kematian terhadap pokok kelapa sawit terjangkit, seterusnya menyebabkan kerugian yang besar kepada ekonomi negara pengeluar. Penggunaan racun kimia juga telah diaplikasikan bagi menangani BSR, namun begitu, kaedah ini tidak mampu mengatasi serangan kulat ini. Beberapa kajian telah dilakukan untuk mengenal pasti potensi penggunaan agen kawalan biologi seperti mikroorganisma antagonis dalam mengawal penyakit BSR. Kajian ini bertujuan untuk mengenal pasti mikroorganisma pencilan tempatan yang mempunyai kesan antagonistik terhadap G. boninense. Sebanyak 49 kultur bakteria tulen telah dipencilkan dari kawasan sekitar Bangi, Selangor, Malaysia. Tiga pencilan iaitu masing-masing dua strain daripada genus Burkholderia dan satu daripada Streptomyces menunjukkan aktiviti antagonistik yang kuat terhadap miselia dwikarion G. boninense strain PER71 dengan nilai Peratusan Perencatan Pertumbuhan Radius (PIRG) melebihi 70% dalam dua asai antikulat yang berbeza. Analisis jujukan DNA bagi semua pencilan telah menunjukkan spesies bakteria tersebut mempunyai kesamaan yang tinggi dengan jujukan nukleotida 16S rRNA daripada Burkholderia stagnalis, B. cepacia dan Streptomyces gelaticus. Bakteria yang digunakan di dalam kajian ini mempunyai potensi yang tinggi untuk digunakan sebagai agen kawalan biologi dalam menghalang penyebaran G. boninense terutamanya di dalam ladang kelapa sawit di Malaysia.

 

Kata kunci: Antagonis; BSR; Burkholderia; kawalan biologi; kulat patogen; Streptomyces

 

ABSTRACT

Basal Stem Rot disease (BSR) caused by the phytopathogenic fungus Ganoderma boninense is a huge threat to the oil palm plantations in Malaysia. BSR can reduce the yield of oil palm fruit bunch and cause fatality on infected oil palm trees thus creating huge economic losses to the producing country. The use of chemical fungicides has been applied in addressing BSR, however these methods were unable to overcome the fungal attack. A few studies have been carried out to identify the potential of biological control agents such as antagonistic microorganisms in controlling BSR disease. This study aimed to identify locally isolated microorganisms having antagonistic effects against G. boninense. A total of 49 pure bacterial cultures were isolated from Bangi, Selangor, Malaysia. Three isolates namely two from genus Burkholderia and one from Streptomyces showed strong antagonistic activity against G. boninense dikaryotic micellia with more than 70% of Percentage Inhibition of Radial Growth (PIRG) in two different antifungal assays. DNA sequence analysis of all isolates showed that the bacteria species shared high similarities in their 16S rRNA sequences with Burkholderia stagnalis, B. cepacia and Streptomyces gelaticus. The bacteria found in this study have high potentials to be used as biological control agents in preventing the spread of G. boninense, particularly in the oil palm plantations in Malaysia.

 

Keywords: Antagonist; biological control; BSR; Burkholderia; fungal pathogen; Streptomyces

 

RUJUKAN

Arif, M.A., Roslan, A., Idris, A.S. & Ramle, M. 2011. Economics of oil palm pests and Ganoderma diseases and yield loses. In Proceedings of the 3rd International Seminar Integrated Oil Palm Pests and Management. MPOB-IOPRI. hlm. 83-98.

Chong, K.P., Dayou, J. & Alexander, A. 2017. Detection and Control of Ganoderma Boninense in Oil Palm Crop. Springer: Berlin. hlm. 1-50.

Eberl, L. & Vandamme, P. 2016. Members of the genus Burkholderia: Good and bad guys. F1000Res. 5: F1000 Faculty Rev-1007.

El Khoury, R., Choque, E., El Khoury, A., Snini, S.P., Cairns, R., Andriantsiferana, C. & Mathieu, F. 2018. OTA prevention and detoxification by actinobacterial strains and activated carbon fibers: Preliminary results. Toxins 10(4): 137.

Esmaeel, Q., Miotto, L., Rondeau, M., Leclère, V., Clément, C., Jacquard, C., Sanchez, L. & Barka, E.A. 2018. Paraburkholderia phytofirmans PsJN-plants interaction: From perception to the induced mechanisms. Front Microbiol. https://doi.org/10.3389/fmicb.2018.02093

Halim, M., Muhaimin, A.M.D., Syarifah-Zulaikha, S.A., Nor Atikah, A.R.M., Masri, M.M. & Yaakop, S. 2017. Evaluation of infestation in parasitoids on Metisa plana Walker (Lepidoptera: Psychidae) in three oil palm plantations in Peninsula Malaysia. Serangga 22(2): 135-149.

Heuer, H., Krsek, M., Baker, P., Smalla, K. & Wellington, E.M. 1997. Analysis of actinomycete communities by specific amplification of genes encoding 16S rRNA and gel-electrophoretic separation in denaturing gradients. Applied and Environmental Microbiology 63(8): 3233-3241.

Hong, C.E. & Park, J.M. 2016. Endophytic bacteria as biocontrol agents against plant pathogens: Current state-of-the-art. Plant Biotechnology Reports 10(6): 353-357.

Hushiarian, R., Yusof, N.A. & Dutse, S.W. 2013. Detection and control of Ganoderma boninense: Strategies and perspectives. SpringerPlus 2(1): 1-12.

Ibrahim, M.S., Abu Seman, I., Rusli, M.H., Izzuddin, M.A., Kamarudin, N., Hashim, K. & Abd Manaf, Z. 2020. Surveillance of Ganoderma disease in oil palm planted by participants of the smallholders replanting incentive scheme in Malaysia. Journal of Oil Palm Research 32(2): 237-244.

Isaac, I.L., Walter, A.W.C.Y., Bakar, M.F.A., Idris, A.S., Bakar, F.D.A., Bharudin, I. & Murad, A.M.A. 2018. Transcriptome datasets of oil palm pathogen Ganoderma boninense. Data in Brief 17: 1108-1111.

Kumar, S., Stecher, G. & Tamura, K. 2016. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33(7): 1870-1874.

Kushairi, A., Ong-Abdullah, M., Nambiappan, B., Hishamuddin, E., Bidin, M., Ghazali, R., Subramaniam, V., Sundram, S. & Parveez, G.K.A. 2019. Oil palm economic performance in Malaysia and R&D progress in 2018. Journal of Oil Palm Research 31(2): 165-194.

Law, J.W.F., Ser, H.L., Ab Mutalib, N.S., Saokaew, S., Duangjai, A., Khan, T.M., Chan, K.G., Goh, B.H. & Lee, L.H. 2019. Streptomyces monashensis sp. nov., a novel mangrove soil actinobacterium from East Malaysia with antioxidative potential. Scientific Reports 9(1): 1-18.

Law, J.W.F., Ser, H.L., Khan, T.M., Chuah, L.H., Pusparajah, P., Chan, K.G., Goh, B.H. & Lee, L.H. 2017. The potential of Streptomyces as biocontrol agents against the rice blast fungus, Magnaporthe oryzae (Pyricularia oryzae). Frontiers in Microbiology https://doi.org/10.3389/fmicb.2017.00003.

Lim, C.K., Ahmad, R., Marzuki, N.F., Goh, Y.K., Azizan, K.A., Goh, Y.K. & Goh, K.J. 2018a. Optimization of metabolite extraction protocols for untargeted metabolite profiling of mycoparasitic Scytalidium parasiticum using LC-TOF-MS. Sains Malaysiana 47(12):  3061-3068.

Lim, P., Gansau, J.A. & Chong, K.P. 2018b. Streptomyces spp. a potential biocontrol agent against Ganoderma boninense of basal stem rot. Journal of Oil Palm Research 30(2): 265-275.

Luqman, H.A., Noor Nasuha, A.A., Dzulhelmi, M.N., Nurul Fatihah, A.L., Muhamad Fahmi, M.H., Teo, T.M., Idris, A.G. & Izfa Riza, H. 2018. Diversity and composition of beetles (Order: Coleoptera) in three different ages of oil palms in Lekir oil Palm Plantation, Perak, Malaysia. Serangga 23(1): 58-71.

Madihah, A.Z., Idris, A.S., Kamaruddin, S., Bharudin, I., Abu Bakar, F.D. & Abd Murad, A.M. 2019. Identification and characterization of a mating signalling gene from an oil palm pathogen, Ganoderma boninense. In AIP Conference Proceedings. AIP. 2111.

Madihah, A.Z., Maizatul-Suriza, M., Idris, A.S., Bakar, M.F.A., Kamaruddin, S., Bharudin, I., Abu Bakar, F.D. & Murad, A.M.A. 2018. Comparison of DNA extraction and detection of Ganoderma, causal of basal stem rot disease in oil palm using loop-mediated isothermal amplification. Malaysian Applied Biology 47(5): 119-127.

Mao, S., Lee, S.J., Hwangbo, H., Kim, Y.W., Park, K.H., Cha, G.S., Park, R.D. & Kim, K.Y. 2006. Isolation and characterization of antifungal substances from Burkholderia sp. culture broth. Current Microbiology 53(5): 358-364.

Maznah, Z., Halimah, M., Ismail, S. & Idris, A.S. 2015. Dissipation of the fungicide hexaconazole in oil palm plantation. Environmental Science and Pollution Research 22(24): 19648-19657.

Mohd-Padil, H., Damiri, N., Sulaiman, S., Chai, S.F., Nathan, S. & Firdaus-Raih, M. 2017. Identification of sRNA mediated responses to nutrient depletion in Burkholderia pseudomallei. Scientific Reports 7(1): 1-11.

Muniroh, M.S., Nusaibah, S.A., Vadamalai, G. & Siddique, Y. 2019. Proficiency of biocontrol agents as plant growth promoters and hydrolytic enzyme producers in Ganoderma boninense infected oil palm seedlings. Current Plant Biology 20: 100116.

Nadarajah, K.K. 2016. Rhizosphere interactions: Life below ground. In Plant-Microbe Interaction: An Approach to Sustainable Agriculture, edited by Choudhary, D.K., Varma, A. & Tuteja, N. Springer: Singapore. https://doi.org/10.1007/978-981-10-2854-0_1.

Naher, U., Sarkar, M.I., Jahan, A., Maniruzzaman, M., Choudhury, A.K., Kalra, N. & Biswas, J. 2019. Nutrient mineralization and soil biology as influenced by temperature and fertilizer management practices. Sains Malaysiana 48(4): 735-744.

Nusaibah, S., Saad, G. & Tan, G.H. 2017. Antagonistic efficacy of Trichoderma harzianum and Bacillus cereus against Ganoderma disease of oil palm via dip, place and drench (DPD) artificial inoculation technique. International Journal of Agriculture and Biology 19(2): 299-306.

Priwiratama, H. & Susanto, A. 2014. Utilization of fungi for the biological control of insect pests and Ganoderma disease in the Indonesian oil palm industry. Journal of Agricultural Science and Technology A 4(2): 103-111.

Ramli, N.R., Mohamed, M.S., Abu-Seman, I., Madihah, A.Z. & Mohamad, N. 2016. The potential of endophytic bacteria as a biological control agent for Ganoderma disease in oil palm. Sains Malaysiana 45(3): 401-409.

Remali, J., Zin, N.M., Ng, C.L., Aizat, W.M. & Tiong, J.J.L. 2019. Fenazin sebagai potensi antibiotik baru daripada Streptomyces kebangsaanensis. Sains Malaysiana 48(3): 543-553.

Retnowati, D., Solihin, D., Ghulamahdi, M. & Lestari, Y. 2018. New information on the potency of sponge-associated actinobacteria as producer of plant growth-promoting bioactive compounds. Malaysian Applied Biology 47(6): 127-135.

Shahbazi, P., Musa, M.Y., Geok, Y.A.T., Avin, F.A., Wee, F.A.T. & Vikineswary, S. 2014. In vitro and in vivo evaluation of Streptomyces suppressions against anthracnose in chili caused by Colletotrichum. Sains Malaysiana 43(5): 697-705.

Shariffah-Muzaimah, S.A., Idris, A.S., Madihah, A.Z., Dzolkhifli, O., Kamaruzzaman, S. & Maizatul-Suriza, M. 2017. Characterization of Streptomyces spp. isolated from the rhizosphere of oil palm and evaluation of their ability to suppress basal stem rot disease in oil palm seedlings when applied as powder formulations in a glasshouse trial. World Journal of Microbiology and Biotechnology 34(1): 15.

Shariffah-Muzaimah, S.A., Idris, A.S., Madihah, A.Z., Dzolkhifli, O. & Kamaruzzaman, S. 2015. Isolation of actinomycetes from rhizosphere of oil palm (Elaeis guineensis Jacq.) for antagonism against Ganoderma boninense. Journal of Oil Palm Research 27(1): 19-29.

Sheibani-Tezerji, R., Rattei, T., Sessitsch, A., Trognitz, F. & Mitter, B. 2015. Transcriptome profiling of the endophyte Burkholderia phytofirmans PsJN indicates sensing of the plant environment and drought stress. MBio 6(5): e00621-15.

Singh, S., Bhatnagar, S., Choudhary, S., Nirwan, B. & Sharma, K. 2018. Fungi as biocontrol agent: An alternate to chemicals. In Fungi and Their Role in Sustainable Development: Current Perspectives, edited by Praveen, G. & Joginder, S. Springer: Singapore. hlm. 23-33.

Sujarit, K., Mori, M., Dobashi, K., Shiomi, K., Pathom-aree, W. & Lumyong, S. 2020. New antimicrobial phenyl alkenoic acids isolated from an oil palm rhizosphere-associated actinomycete, Streptomyces palmae CMU-AB204T. Microorganisms 8(3): 350.

 

*Pengarang untuk surat-menyurat; email: ibb@ukm.edu.my

 

   

   

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