The
Malaysian Journal of Analytical Sciences Vol 10 No 2 (2006): 205 – 210
COMPARATIVE SENSITIVITIES OF CHOLESTEROL ANALYSIS
USING GC, HPLC AND SPECTROPHOTOMETRIC METHODS
Osman, H.* & Yap Kwee Chin
School of
Chemical Sciences and Food Technology
Faculty of
Science and Technology, Universiti Kebangsaan Malaysia,
43600 UKM Bangi,
Selangor
*Corresponding
author: osman@ukm.my
Abstract
Three
methods of extraction namely Bohac ( 1984 ), Beyer & Jensen ( 1989) and
Queensland Health Science Institute, Australia (QSE-CAM-004) (1995) were
compared for the extraction of cholesterol from egg yolk. Each of the extracted
samples was then analyzed by using Spectrophotometer, HPLC and GC respectively.
The results were then evaluated for their analytical linearity, accuracy,
precision, percentage of recovery and the effects of sample sizes. In this
study, the results showed that HPLC system was found to be more suitable in
determination of cholesterol with the limit of detection of 0.08 µg cholesterol/mL,
precision of 97.5% and coefficient variation of 4.94%. By using cholesterol
standard in the comparison of methods, Bohac extraction method was found to be
the most effective, with the percentage of recovery of 96.53%, linearity of
r2>0.99 and effective range of 0.0 - 0.75mg/mL. The Bohac extraction method
and HPLC were the best combination for extraction and analysis of cholesterol.
Keywords: Cholesterol
analysis, methods of extraction, methods of analaysis, LOD, LOQ
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