The Malaysian Journal of Analytical Sciences Vol 12 No 3 (2008): 586 - 592

 

 

 

ELECTROCHEMICAL PREANODIZATION OF GLASSY CARBON ELECTRODE AND APPLICATION TO DETERMINE CHLORAMPHENICOL

 

Nguyen Minh Truc^*, John Mortensen and Nguyen Ba Hoai Anh

 

Department of Anal. Chem., Faculty of Chemistry, Ho Chi Minh University of Natural Sciences,

HCMC, Viet Nam.

 

*Corresponding author: nmtruc@hcmuns.edu.vn

 

Abstract

This paper suggested a method to enhance the performance of carbon electrodes for the determination of chloramphenicol (CAP). The sensitivity and  the reproducibility of the carbon electrodes could be  enhanced easily by electrochemical pretreatment. Some kinds of carbon material were studied including glassy carbon, graphite carbon and pyrolytic carbon. Numerous kinds of supporting electrolyte  have been tried. For glassy carbon electrode, the acidic solution, H2SO4   5mM, resulted in best performance at pretreated voltage of +2.1V (vs. Ag/AgCl) in duration of 250 second. However, for graphite and pyrolytic carbon electrodes, the phosphate buffer solution pH 6.0 gave the best performance at +1.7V (vs. Ag/AgCl) in duration of 20 seconds. The detection limit could be at very low concentration of CAP: 0.8ng/ml for glassy carbon electrode, 3.5ng/ml for graphite carbon electrode. The method was successful applied to aqua-agriculture water  sample and milk sample with simple extraction as well as direct ointment sample analysis.

 

Keywords : Chloramphenicol, electrochemical pretreatment, voltammetry, glassy carbon electrode

 

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