The Malaysian Journal of Analytical Sciences Vol 12 No 3 (2008): 552 - 560

 

 

ANALYSIS OF HUMAN GALLSTONES BY FTIR

 

Naseem A. Channa¹*, Fateh D. Khand², and Muhammad Iqbal Bhanger³

 

¹Institute of Biochemistry, University of Sindh, Jamshoro

²Department of Biochemistry, isra University, Hyderabad

³National Center of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, Pakistan

 

*Corresponding author: nachanna2000@yahoo.com

 

Abstract

The present study was aimed at determining the composition of gallstones removed from patients  in  Southern Sindh, Pakistan. 109 gallstone samples surgically removed from as many patients (98 females and 11 males; age range 20 to 80 years) admitted for treatment in Liaquat University hospital, Jamshsoro during 2000 to 2003, were analyzed for composition by Fourier Transform Infrared (FTIR) spectroscopy. 74  (67.9%) of the 109 gallstone samples were found to be pure cholesterol  stones,  5  (4.6%)  pure  calcium carbonate stones,  13  (11.9%) cholesterol +  calcium carbonate,  10  (9.2%) cholesterol  +  bilirubin  and  07  (6.4%)  calcium  bilirubinate  stones.  In  mixed  composition  gallstones  cholesterol  was concentrated more at periphery than in the center of stone. Cholesterol either singly (67.9%) or in combination with either calcium carbonate (11.9%) or bilirubin (9.2%) was the most predominant component of gallstones. Analysis of gallstones based on FTIR suggests that cholesterol either singly or in combination with either calcium carbonate or bilirubin is the most predominant component of gallstones from Southern Sindh, Pakistan.

 

Keywords: Gallstones, Composition, FTIR, Cholesterol, Bilirubin, Calcium bilirubinate, Calcium carbonate,

mixed gallstones.

 

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