The Malaysian Journal of Analytical Sciences Vol 16 No 2(2012): 172 – 179

 

 

 

ANALISIS KROMATOGRAFI GAS x KROMATOGRAFI GAS–SPEKTROMETRI JISIM MASA-PENERBANGAN

MINYAK PATI GLOBBA PATENS VAR. PATENS

 

(Analysis of Gas Chromatography x Gas Chromatography–Time-of-Flight Mass Spectrometry of Essential Oils from Globba patens var. patens)

 

A. Masila¹, W.A. Yaacob^1*, I. Nazlina²

 

¹Pusat Pengajian Sains Kimia dan Teknologi Makanan,

²Pusat Pengajian Biosains dan Bioteknologi,

Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia,

43600 Bangi, Selangor, Malaysia

 

*Corresponding author:wanyaa@ukm.my

 

 

Abstrak

Minyak pati daun, batang, dan rizom Globba patens var. patens (Zingiberaceae) yang diperolehi dengan penyulingan-air adalah 0.0021%, sedikit, dan juga sedikit. Komponen kimia mereka dan peratus masing-masing ditentukan dari analisis dengan kromatografi gasxkromatografi gas–spektrometri jisim masa-penerbangan (KGxKG-SJMP). Terpena adalah penyumbang utama minyak dengan rizom memberikan amaun terendah 30.56%. Sebatian utama dalam minyak daun adalah β-tujena (11.45%, 1),  β-selinena (7.82%, 2), dan α-pinena (5.27%, 3); batang, β-pinena (2.77%, 4), mirtenol (2.30%, 5), dan β-elemena/ledena oksida (2.23%, 6/7); rizom, kariofilena oksida (2.74%, 8), D-limonena (2.71%, 9), dan 7-(1-metiletilidena)-bisiklo[4.1.0]heptana (1.93%, 10).

 

Kata kunci: Globba patens var. patens, minyak pati, KGxKG-SJMP

 

Abstract

Leaf, stem, and rhizome essential oils of Globba patens var. patens (Zingiberaceae) were obtained by hydrodistillation at 0.0021%, in small quantity, and also small quantity. Their chemical components and each percentage were determined from analysis by using gas chromatographyxgas chromatography–time-of-flight mass spectrometry (GCxGC-TOFMS). Terpenes were the oils major contributor with the rhizome gave the lowest amount of 30.56%. Major compounds in the leaf oil were β-thujene (11.45%, 1),  β-selinene (7.82%, 2), and α-pinene (5.27%, 3); the stem, β-pinene (2.77%, 4), myrtenol (2.30%, 5), and β-elemene/ledene oxide (2.23%, 6/7); the rhizome, caryophyllene oxide (2.74%, 8), D-limonene (2.71%, 9), and 7-(1-methylethylidene)-bicyclo[4.1.0]heptane (1.93%, 10).  

 

Keywords: Globba patens var. patens, essential oil, GCxGC-TOFMS

 

References

1.       Habsah, M., Ali, A.M., Lajis, N.H., Sukari, M.A., Yap, Y.H., Kikuzaki, H., Nakatani, N. 2005. Antitumor promoting and cytotoxic constituents of Etlingera elatior. Malaysian Journal of Medical Sciences. 12(1): 6-12.

2.       Zakaria, M.B. & Ibrahim, H. 1986. Phytochemical screening of some Malaysian species of Zingiberaceae. Malaysian Journal of Science 8: 125-128.

3.       Takano, A., Hernawati & Tamin, R. 2001. Notes on Globba Linnaeus in Sumatra, W. Malaysia & Borneo: a new record of Globba brachyanthera var. rubra in Sumatra. Folia Malaysiana 2(1): 25-34.

4.       Larsen, K., Ibrahim, H., Khaw, S.H. & Saw, L.G. 1999. Gingers of Peninsular Malaysia and Singapore. Kota Kinabalu: Natural History Publications (Borneo) Sdn. Bhd.

5.       Ibrahim, H. & Rahman, A.A. 1988. Several ginger plants (Zingiberaceae) of potential value. Proceeding of the Seminar on Malaysian Traditional Medicine, hlm. 159-161. Kuala Lumpur: IPT, University Malaya.

6.       Dallüge, J., van Stee, L.L.P., Xu, X., Williams, J., Beens, J., Vreuls, R.J.J. & Brinkman, U.A.T. 2002. Unravelling the composition of very complex samples by comprehensive gas chromatography coupled to time-of-flight mass spectrometry. cigarette smoke. Journal of Chromatography, A 974(1-2): 169-184.

7.       Raj, G., George, V., Dan, M. & Sethuraman, M.G. 2010. Essential oil composition of Globba schomburgkii Hook. f. and Globba ophioglossa Wight. Journal of Essential Oil Research 22(3), 220-222.

8.       Menon, A.N. & Dan, M. 2009. Chemical composition of essential oils of Globba species from south India. Journal of Essential Oil Research 21(1), 59-60.