Malaysian
Journal of Analytical Sciences Vol 21 No 2 (2017): 283 - 290
DOI:
https://doi.org/10.17576/mjas-2017-2102-02
GLUCOSE PRODUCTION FROM STEAM-ALKALI-CHEMICAL
PRE-TREATED OIL PALM TRUNK BIOMASS VIA ENZYMATIC
SACCHARIFICATION PROCESS
(Penghasilan Glukosa dari Pra-Rawatan
Wap-Kimia-Alkali Biomas Batang Kelapa Sawit Melalui Proses Sakarifikasi Enzim)
Nurul Wahidah Mazlan, Long
Wee Lai*, Nur Akmal Suliman, Marini Ibrahim, Rahmad Mohd Taib, Rozila Alias
Department of Science and Biotechnology,
Faculty of Engineering and Life Sciences,
Universiti Selangor, Jalan Timur Tambahan, 45600
Bestari Jaya, Selangor, Malaysia
*Corresponding author: zki@unisel.edu.my
Received: 7
June 2016; Accepted: 1 February 2017
Abstract
This
study proposed lignocellulosic oil palm trunk (OPT) biomass can be used as
alternative substrate for glucose emanation via
enzymatic saccharification route. The OPT biomass was pre-treated using
steam-alkali-chemical (SAC) method prior to enzymatic saccharification process
for glucose formation. Three basic physiochemical parameters such as enzyme
concentration (20 – 100 FPU.mL-1), pH (4.0 – 8.0) and reaction
temperature (30 – 60 °C) were intensively studied. Results revealed that all
parameters gave significant effect on glucose production. By setting the enzyme
hydrolysis conditions at pre-determined parameters points, i.e. enzyme
concentration, 60 FPU.mL-1; pH, 6.0 and reaction temperature, 50 °C,
respectively; 4.27 g.L-1 of glucose was attained at 72 hours
hydrolysis. There is 4.49-fold of glucose increment in SAC-treated OPT
substrate compared to untreated ones. This research also indicates enzyme
digestibility can be enhanced by using treated OPT as substrate.
Keywords: oil palm trunk
biomass, steam-alkali-chemical pre-treatment, enzymatic saccharification,
cellulase enzyme
Abstrak
Kajian ini mencadangkan
lignoselulosa biomas batang kelapa sawit (OPT) boleh digunakan sebagai
alternatif substrat bagi penghasilan glukosa melalui kaedah enzim sakarifikasi.
Biomas OPT terlebih dahulu dirawat dengan wap-kimia-alkali (SAC) sebelum proses
sakarifikasi enzimatik bagi pembentukan glukosa. Tiga fizikokimia
parameter asas seperti kepekatan enzim (20 – 100 FPU.mL-1), pH (4.0 – 8.0) dan suhu tindak-balas (30 – 60 °C)
telah dikaji secara intensif. Hasil kajian menunjukkan bahawa semua parameter
memberi kesan ke atas pengeluaran glukosa. Dengan menetapkan syarat enzim
hidrolisis pada parameter yang telah ditentukan, iaitu masing-masing; kepekatan
enzim, 60 FPU.mL-1; pH, 6.0 dan suhu tindak-balas, 50 °C; 4.27 g.L-1 glukosa telah dicapai pada 72 jam hidrolisis. Glukosa
meningkat sebanyak 4.49 kali ganda dalam SAC-rawatan OPT substrat berbanding
yang tidak dirawat. Kajian ini juga membuktikan penghadaman enzim boleh
dipertingkatkan dengan menggunakan OPT terawat sebagai substrat.
Kata kunci: biomas batang kelapa sawit, pra-rawatan
wap-kimia-alkali, sakarifikasi enzimatik, enzim selulase
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