Malaysian Journal of Analytical Sciences Vol 21 No 2 (2017): 298 - 303

DOI: https://doi.org/10.17576/mjas-2017-2102-04

 

 

 

ISOLATION OF FOUR STEROIDS FROM THE LEAVES OF FERN Adiantum latifolium Lam

 

(Pengasingan Empat Steroid Daripada Daun Paku Pakis Adiantum latifolium Lam)

 

Andi Rifki Rosandy¹, Nurnida Mohd. Kamal², Noraini Talip², Rozida Khalid¹, Muntaz Abu Bakar¹*

 

¹School of Chemical Sciences and Food Technology, Faculty of Science and Technology

²School of Environmental and Natural Resources Sciences, Faculty of Science and Technology

Universiti Kebangsaan Malaysia, 43650 UKM Bangi, Selangor, Malaysia

 

*Corresponding author: muntaz@ukm.edu.my

 

 

Received: 1 August 2016; Accepted: 27 February 2017

 

 

Abstract

Isolation of compounds from methanol extract from the leaves of Adiantum latifolium Lam. had been conducted by using radial chromatography and thin layer chromatography (TLC) techniques. Their structures were elucidated by ultraviolet-visible (UV), infrared (IR), nuclear magnetic resonance (NMR) and mass spectroscopy (MS) and by comparison with the previous literatures. Four steroids compound were isolated namely the Stigmast-4-en-3-one (1), Stigmasta-4,22-dien-3-on (2), Stigmasterol (3) and β-Sitosterol (4).

 

Keywords:  Adiantum latifolium, adiantaceae, steroid

 

Abstrak

Pengasingan sebatian daripada ekstrak metanol daripada daun Adiantum latifolium Lam. telah dilakukan dengan menggunakan teknik kromatografi pepejal dan teknik kromatografi lapisan nipis (TLC). Struktur semua sebatian dicirikan dengan menggunakan ultra lembayung (UV), inframerah (IR), resonans magnet nukleus (NMR) dan spektroskopi jisim (MS), dan secara perbandingan dengan data kepustakaan. Empat sebatian steroid telah diasingkan iaitu, Stigmasta-4-en-3-on (1), Stigmasta-4,22-dien-3-on (2), Stigmasterol (3) and β-Sitosterol (4).

 

Kata kunci:  Adiantum latifolium, adiantaceae, steroid

 

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