Malaysian Journal of Analytical Sciences Vol 21 No 6 (2017): 1389 - 1398

ISOLATION OF TWO BOTRYANES FROM Hypoxylon rickii AND IDENTIFICATION OF THE ENCODING GENES

(Pemencilan Sebatian Botrien dari Hypoxylon rickii dan Pengenalpastian Pengekodan Gen)

Afnani Alwi¹, Andi Rifki Rosandy², Farah Diba Abu Bakar¹, Rozida Mohd Khalid²*

¹School of Biology and Biological Sciences, Faculty of Science and Technology

²School of Chemical Sciences and Food Technology, Faculty of Science and Technology

Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia

*Corresponding author: rozidakhalid@ukm.edu.my

Received: 28 September 2016; Accepted: 6 March 2017

Abstract

10-oxodehydrobotrydial (1) and 4β-Acetoxy-9β,10β,15α-trihydroxyprobotrydial (2) have been isolated from Hypoxylon rickii mycelia extract. Compound 2 is probably an intermediate of botrydial, which is a known sesquiterpene phytotoxin. Cluster Omega and Artemis software analysis suggested that HRT6, a possible botrydial-like gene cluster with four genes shows more than 50% similarities compared to Botrytis cinerea botrydial gene cluster, BcBOT. A comparison between proposed 4β-Acetoxy-9β,10β,15α- trihydroxyprobotrydial (2) producing pathway and BcBOT proposed pathway shows compatible function of each gene prediction. Further confirmation using RNA gene knock-outs is on-going.

Keywords: Hypoxylon rickii, botryane, terpene synthase

Abstrak

10-oksodehidrobotridial (1) dan 4β-Asetoksi-9β,10β,15α-trihidroksiprobotridial (2) telah dipencilkan dari ekstrak miselia Hypoxylon rickii. Sebatian 2 dijangkakan sebagai sebatian perantara dalam pembentukkan botridial iaitu sejenis fitotoksin sesquiterpena. Penggunaan dua perisian Cluster Omega dan Artemis telah berjaya mengenalpasti gen kelompok yang berkemungkinan dari H. rickii sebagai HRT6 dengan empat gen menunjukkan persamaan lebih daripada 50% berbanding gen kelompok Botrytis cinerea, BcBOT. Perbandingan antara dengan cadangan laluan biosintesis 4β-Asetoksi-9β,10β,15α-trihidroksiprobotridial (2) dengan BcBOT menunjukkan persamaan fungsi – fungsi gen. Pengesahan lanjut menggunakan RNA (mendiamkan gen) sedang dijalankan.

Kata kunci: Hypoxylon rickii, botrien, terpene sintes

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