Malaysian Journal of Analytical Sciences Vol 22 No 6 (2018): 1078 - 1083

DOI: 10.17576/mjas-2018-2206-18

 

 

 

In-vitro PHOTOPROTECTIVE ACTIVITIES OF DIFFERENT SOLVENT EXTRACTION OF Hibiscus sabdariffa

 

(In-vitro Aktiviti Foto Perlindungan Bagi Ekstrak Pelarut yang Berbeza dari Hibiscus sabdariffa)

 

Nur Royhaila Mohamad¹, Siti Salwa Abd Gani^1,2*,  Roswanira Abdul Wahab³, Uswatun Hasanah Zaidan⁴

 

¹Halal Product Research Institute (HPRI), Putra Infoport

²Department of Agriculture Technology, Faculty of Agriculture

Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia

³Department of Chemistry, Faculty of Science,

Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Malaysia

⁴Department of Biochemistry, Faculty of Biotechnology & Biomolecular Sciences,

Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia

 

*Corresponding author:  ssalwaag@upm.edu.my

 

 

Received: 2 April 2018; Accepted: 31 July 2018

 

 

Abstract

In recent years, plant extracts have been considered as potential sunscreen resources owing to their antioxidant activity as well as capability to absorb ultraviolet (UV) rays in the UVA region. In the present study, the UV protective factor of three different extracts of Hibiscus sabdariffa (HS) namely ethanol (HS-ETOH), ethyl acetate (HS-EA), and hexane (HS-HEX), all of which are rich in flavonoids and other phenolic components were assessed by using UV-Vis spectrophotometer to estimate their corresponding in-vitro Sun Protection Factors (SPF). The results revealed that both extracts of HS-ETOH and HS-EA showed characteristic absorption bands in the UVA and UVB regions, while the HS-HEX extract was observed to absorb in the UVB and UVC regions under similar concentration. Pertinently, the highest SPF (17.53±0.22) was obtained in the HS-ETOH extract, inferring its potential as an active component for sunscreen in pharmaceutical and cosmeceutical preparations.

 

Keywords:  Hibiscus sabdariffa, sun protection factor, sunscreen, UV-spectrophotometry

 

Abstrak

Bahan-bahan semulajadi yang telah diekstrak daripada tumbuh-tumbuhan baru-baru ini telah dipertimbangkan potensinya untuk menjadi sumber perlindungan matahari bagi penyerapan sinar ultraviolet dalam lingkungan UVA dan aktiviti antioksidan. Dalam kajian ini, faktor perlindungan UV bagi tiga ekstrak yang berbeza iaitu ekstrak etanol (HS-ETOH), ekstrak etil asetat (HS-EA), dan ekstrak heksana (HS-HEX) daripada Hibiscus sabdariffa (HS) yang kaya dengan komponen seperti flavonoid dan fenolik telah dinilai  dengan menggunakan spektrofotometer UV-Vis dan dikira dengan Faktor Perlindungan Matahari (SPF). In-vitro SPF daripada ekstrak HS telah dianalisis dengan menggunakan pengukuran penjerapan terhadap pelarut yang berbeza semasa proses pengekstrakan. HS-ETOH dan HS-EA telah menunjukkan ciri jalur penyerapan bagi kawasan UVB dan UVA manakala HS-HEX di kawasan UVC dan UVB pada kepekatan yang sama. HS-ETOH mencapai faktor perlindungan matahari yang paling tinggi SPF (17.53±0.22). Ia menunjukkan kemungkinan untuk menggunakan ekstrak ini sebagai perlindungan matahari dalam persediaan farmaseutikal dan kosmeseutikal.

 

Kata kunci:  Hibiscus sabdariffa, faktor perlindungan matahari, pelindung matahari, UV-spektrofotometri

 

References

1.       Vinay, K. G., Rajbir, K., Ramit, S. and Vikas, J. (2016). Photoprotective, antioxidant screening and new ester from dry root extracts of Potentilla atrosanguinea (Himalayan cinquefoil). South African Journal of Botany, 103: 49-53.

2.       Dutra, E. A., Oliveira, D. A., Kedor-Hackmann, E. R. and Santoro, M. I. (2004). Determination of sun protection factor (SPF) of sunscreens by ultraviolet spectrophotometry. Brazilian Journal of Pharmaceutical Science, 40: 381-385.

3.       Mambro, V. M. D. and Fonseca, M. J. V. (2005). Assays of physical stability and antioxidant activity of a topical formulation added with different plant extracts. Journal of Pharmaceutical and Biomedical Analysis, 37: 287-295.

4.       Mohamed, R., Fernandez, J., Pineda, M. and Aguilar, M. (2007). Roselle (Hibiscus sabdariffa) seed oil is a rich source of gamma-tocopherol. Journal of Food Science, 72(3): 207-211.

5.       Mourtzinos, I., Makris, D. P., Yannakopoulou, K., Kalogeropoulos, N., Michali, I. and Karathsnos, V. T. (2008). Thermal stability of anthocyanin extract of Hibiscus sabdariffa L. in the presence of β-cyclodextrin. Journal of Agricultural and Food Chemistry, 56: 10303-10310.

6.       Da-Costa-Rocha, I., Bernd, B., Hartwig, S., Ivo, P. and Micheal, H. (2014). Hibiscus Sabdariffa L- a phytochemical and pharmacological review. Food Chemistry, 165: 424-443.

7.       Violante, I. M. P., Souza, I. M., Venturini, C. L., Ramalho, A. F. S., Santos, R. A. N. and Ferrari, M. (2009). Avaliação in vitro da atividade fotoprotetora de extratos vegetais do cerrado de Mato Grosso. Brazilian Journal of Pharmacognsosy,19: 452-457.

8.       Zhen, J., Villani, T. S., Guo, Y. Q. Y., Chin, K., Min-Hsiung, P., Chi-Tang, H., James, E. S. and Qingli W. (2016). Phytochemistry, antioxidant capacity, total phenolic content and anti-inflammatory activity of Hibiscus sabdariffa leaves. Food Chemistry, 190: 673-680.

9.       Mansur, J. S., Breder, M. V. R., Mansur, M. C. A. and Azulay, R. D. (1986). Determinação do fator de proteção solar por espectrofotometria. Anais Brasileiros de Dermatologia, 61: 121-124.

10.    Sayre, R. M., Agin, P. P., Levee, G. J. and Marlowe, E. (1979). Comparison of in vivo and in vitro testing of sun screening formulas. Photochemistry and Photobiology, 29: 559-566.

11.    Riegelman, S. and Penna, R. P. (1960). Effect of vehicle components on the absorption characteristics of sunscreens compounds. Journal of Society Cosmetic Chemist, 11: 280-291.

12.    Agrapidis-Paloympis, L. E., Nash, R. B. and Shaath, N. A. (1987). The effect of solvents on the ultraviolet absorbance of sunscreens. Journal of Society Cosmetic Chemist, 38: 209-221.

13.    Suva, M. A. (2014). Evaluation of sun protection factor of Zingiber officinale roscoe extract by ultraviolet spectroscopy method. Journal of Pharmaceutical Science and Technology, 3: 95-97.

14.    Roy, A., Sahu, R. K., Matlam, M., Deshmukh, V. K., Dwivedi, J. and Jha, A. K. (2013). In vitro techniques to assess the proficiency of skin care cosmetic formulations. Pharmacognosy Reviews, 7: 97-106.

15.    Treichel, H., De Oliveira, D. A., Mazutti, M., Di Luccio, M. and Oliveira, J. V. (2010) A review on microbial lipases production. Food Bioprocess Technology, 3: 182-196.

16.    Kaur, C. D. and Saraf, S. (2011). Photochemprotective activity of alcoholic extracts of Camellia sinensis. International Journal of Pharmacology, 7(3): 400-404.

17.    Imam, S., Azhar, I. and Mahmood, Z. A. (2015). In-vitro evaluation of sun protection factors of a cream formulation prepared from extracts of Musa accuminata (L.), Psidium gujava (L.) and Pyrus communis (L.). Asian Journal of Pharmaceutical and Clinical Research, 8(3); 234-237.