Malaysian Journal of Analytical Sciences Vol 23 No 1 (2019): 116 - 123

DOI: 10.17576/mjas-2019-2301-14






(Pengoptimuman Penghasilan Hidrogen dari Buangan Buah melalui Fermentasi Tanpa Cahaya pada Kondisi Mesofilik dan Termofilik: Pengaruh Nisbah Substrat-Inokulum)


Khamdan Cahyari1,2*, Muslikhin Hidayat1, Siti Syamsiah1, Sarto1


1Department of Chemical Engineering, Faculty of Engineering,

Gadjah Mada University, Indonesia

2Dept. of Chemical Engineering, Faculty of Industrial Technology,

Universitas Islam Indonesia, Indonesia


*Corresponding author: 



Received: 13 April 2017; Accepted: 17 April 2018




This research was aimed to optimize hydrogen production from fruit waste, particularly on the effect of the substrate-to-inoculum ratio (SIR). Production of hydrogen was carried out through dark fermentation process both in mesophilic (30 °C, 1 atm) and thermophilic (55 °C, 1 atm) condition. Fermentation was conducted at SIR value ranging from 0.800 to 174 VSsubstrate/g VSinoc. In mesophilic fermentation, the highest cumulative total gas yield was achieved at SIR value of 19 corresponding total gas yield of 113 ml STP/g VS (5%v of H2). In thermophilic condition, the highest H2 yield was obtained at SIR value of 0.800 VSsubstrate/g VSinoc with H2 yield of 294 mL STP/g VS (50 – 60%v of purity). It was noticed that the lower SIR value, the higher hydrogen yield. In summary, it is concluded that substrate-to-inoculum ratio (SIR) plays important role in dark fermentation process to produce renewable energy of hydrogen fuel.


Keywords:  hydrogen, fermentation, substrate-to-inoculum ratio, fruit waste, renewable energy



Kajian ini merupakan langkah pengoptimuman penghasilan hidrogen dari bahan buangan buah, khususnya pada pengaruh nisbah substrat-inokulum (NSI). Penghasilan hidrogen dilakukan melalui proses fermentasi tanpa cahaya pada kondisi mesofilik (30 °C, 1 atm) dan termofilik (55 °C, 1 atm). Fermentasi dilakukan dengan variasi nilai NSI antara 0,800 dan 174 g VSsubstrate/g VSinoc. Pada fermentasi mesofilik, hasil gas total kumulatif tertinggi diperoleh pada nilai NSI 19 g VSsubstrate/g VSinoc dengan nilai penghasilan gas sebesar 113 ml STP/g VS (5%v/v gas H2). Sedangkan proses fermentasi termofilik, hasil hidrogen kumulatif tertinggi dicapai pada nilai RSI 0,800 VSsubstrate/g VSinoc sebesar 294 ml STP H2/g VS (ketulenan H2 50-60%v/v). Hal ini menunjukkan bahawa semakin kecil nilai NSI, hasil gas hidrogen menjadi semakin besar. Sehingga dapat disimpulkan bahawa faktor nisbah substrat terhadap inokulum (NSI) memiliki peranan penting dalam proses fermentasi tanpa cahaya untuk menghasilkan sumber tenaga baharu hidrogen.


Kata kunci:  hidrogen, fermentasi, nisbah substrat-inokulum, bahan buangan buah, tenaga baharu



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