Secretion of RANTES and Interleukin-8by the Human Gastric Epithelial Cell Line (MKN45) through Modulation with Cytokines. NORINA ABDULLAH, JORDAN N. J., KOLIOS, G. & COLLINS A. J. Jurnal Sains Kesihatan Malaysia 1 Julai/July 2003

Abstract
In this in vitro study, the secretion of interleukin-8 (IL-8) and RANTES proteins by the MKN45 gastric epithelial cell line was examined using the ELISA technique after the cells had been stimuylated with pro-inflammatory cytokines, i.e. IL-1 a, TNF- a dan IFN-g. The chemokines demonstrated a time-dependent increase in concentration after stimulation with the various cytokines, alone or in combination, IL-1 a, TNF-a and IFN-g provided the strongest stimulation. The immunomodulatory effects of IL-4 and IL-13 on the chemokine protein secretion were also studied. Stimulation of the cells with IL-4 and IL-13 for an hour at relatively high concentrations (10 and 30 ng/ml) prior to stimulation with the combination IL-1 a, TNF-a dan IFN-g inhibited the secretion of RANTES protein but did not inhibit the secretion of IL-8 mRNA or pathways. IL-4 and IL-13 played an important role in decreasing certain inflammatory chemokines and this might have an important effect in decreasing gastric inflammation.

Key words: chemokines, interleukin-8, RANTES, cytokines, IL-4, IL-13, MKN45 gastric epithelial cell