The Establishment of a Primary Colon Carcinoma Cell Line. ZUBAIDAH ZAKARIA, GREGORY, A. R. A., TONG, S., BOON, C. F., ASRIL YUSOF, NORHANOM ABDUL WAHAB, LATIFAH SAIFUL YAZAN, MUHAMMAD SAIFULAMMAN MOHAMED SAID. Jurnal Sains Kesihatan Malaysia 1 Julai/July 2003

Abstract
A primary human colon carcinoma cell line (IMR22) was established in the Institute for Medical Research from a 71 years old Chinese man who had a right Hemicolectomy done for a fungating mass in the caecum. The tumour mass was then confirmed histopathologically to be a well differentiated Adenocarcinoma Dukes Stage B. A piece of the tumor tissue resected fresh from the operating theatre was grown to establish the cell line . The initial growth medium was in Amniomax C 100 supplemented with Fungizone, Penicillin and Streptomycin and was later changed to Medium 199 with Earl’s salt supplemented with 20% fetal calf serum, Kanamycin, Penicillin and Streptomycin and L-glutamine. The IMR22 line was established after ten passages with the occurrence of single type of cells in the culture whose morphological characteristics resembled that of the primary tumour.

Key words: Human colon carcinoma cell line, tissue culture, histophatology, growth medium, primary tumor