Dalam kajian retrospektif limfoma ini, kandungan DNA dan indeks proliferasi daripada 37 kes limfoma yang disahkan secara histopatologi yang terdiri daripada 29 lelaki dan 8 perempuan diukur menggunakan flowsitometer. Ini dilakukan dengan menggunakan ‘embedded paraffin tissue’ daripada kes yang diambil dari Bahagian Patologi Hospital Kuala Lumpur dalam jangka masa 1990-1995. Di antara mereka, 24 kes didiagnos sebagai limfoma bukan Hodgkin (NHL) manakala 13 kes lagi sebagai penyakit Hodgkin (HD). Nodus limfa yang menunjukkan perubahan reaktif serta pembuangan tonsil bagi inflamasi kronik digunakan sebagai kawalan. Daripada semua kes yang dikaji bagi NHL (Sel B = 70.8%, Sel T = 8.3% dan kes yang tidak dapat dikenalpasti=20.9%), 12.5% adalah diploid (Indeks DNA, DI = 1.0 - 1.10), 66.7% adalah hiperdiploid (DI = 1.10 - 1.85) dan 20.8% adalah hipodiploid (DI < 1.0). Majoriti kes bagi hiperdiploid adalah sel B jenis NHL. Dalam HD (keberselan bercampur = 1, nodul sklerosis = 5, limfosit predominan = 1 dan tidak diklasifikasikan = 6), 30.8% adalah diploid, 46.1% adalah hiperdiploid dan 23.1% adalah hipodiploid. Penemuan signifikan dalam kajian ini adalah majoriti NHL dan HD menunjukkan indeks proliferasi yang tinggi.

Kata kunci: Analisis DNA, flowsitometri, limfoma Hodgkin, indeks DNA, indeks proliferasi.

In this retrospective study of lymphomas, DNA content and proliferative index of 37 histopathologically confirmed cases of lymphoma consisting of 29 male and 8 female were measured using a flowcytometer. This was carried out using paraffin embedded tissues of cases diagnosed in the Department of Pathology, Hospital Kuala Lumpur, over a period of 1990 to 1995. Among them, 24 were diagnosed to be non-Hodgkin’s lymphomas (NHL) while 13 were diagnosed as Hodgkin’s disease (HD). Lymph nodes showing reactive changes and tonsils removed for chronic inflammation were used as control. Of all the cases studied for NHL (B cell = 70.8%, T cell = 8.3% and unclassified cases = 20.9%), 12.5% were near diploid (DNA index, DI = 1.0 - 1.10), 66.7% were hyperdiploid (DI = 1.10 - 1.85) and 20.8% were hypodiploid (DI < 1.0). Majority of the hyperdiploid cases were B cell type of NHL. In HD (mixed cellularity = 1, nodular sclerosing = 5, lymphocyte predominant = 1 and unclassified = 6) 30.8% were near diploid, 46.1% were hyperdiploid and 23.1% were hypodiploid. The significant finding of this study was that the majority of the NHL and HD showed a high proliferative index.

Key words: DNA analysis, flowcytometry, Hodgkin’s lymphoma, DNA index, proliferative index.