Jurnal Sains Kesihatan Malaysia 2 (1) 2004
Komunikasi Pendek/Short Communications
Inhibitory Effects of the Extracts of Plantago major Linn. on the Number and Size of Calcium Oxalate Crystals in vitro.
SHARIFA ABDUL AZIZ, SUHANA MD SAAD, KHAIROL OSMAN,
KHARTINI ABDUL WAHAB & MOHD AZMAN ABU BAKAR
ABSTRAK
Kajian ini dijalankan untuk menentukan kesan aktiviti perencatan pelbagai ekstrak Plantago major ke atas kristal kalsium oksalat secara in vitro. Ekstrak, petroleum eter, n-butanol, etanol, metanol dan akues P. major digunakan pada kepekatan masing-masing10 mg/ml dan dimetilsulfoksida digunakan sebagai kawalan negatif dan zilorik sebagai kawalan positif. Kaedah slaid gel Schneider digunakan dan pengukuran bilangan dan saiz kristal kalsium oksalat (dihidrat dan monohidrat) dilakukan setiap 24 jam dengan menggunakan sistem Analisa Imej. Saiz kristal kalsium oksalat dihidrat menurun secara signifikan (p < 0.05) pada semua ekstrak kecuali petroleum eter berbanding dengan kawalan. Saiz kristal monohidrat menurun secara signifikan (p < 0.05) pada ekstrak n-butanol dan akues sahaja. Saiz kristal monohidrat menurun secara signifikan (p < 0.05) pada ekstrak n-butanol dan akues berbanding dengan zilorik. Bilangan kristal monohidrat menurun secara signifikan (p < 0.05) pada ekstrak etanol, n-butanol dan akues berbanding dengan kawalan dan etanol (juga dihidrat) dan akues berbanding dengan zilorik. Hasil ini menunjukkan kebanyakan ekstrak P. major berupaya merencat saiz dan bilangan kristal kalsium oksalat secara in vitro pada kepekatan 10 mg/ml selepas 24 jam.
ABSTRACT
This study was carried out to determine the inhibitory effects of the various extracts of Plantago major on calcium oxalate crystals in vitro. The petroleum ether, n-butanol, ethanol, methanol and aqueous extracts of P. major were each used at a concentration of 10 mg/ml and dimethylsulfoxide (
DMSO) was used as a control. Modified Schneider slide gel method was used and the number and size of calcium oxalate crystals (both dihydrate and monohydrate types) were counted every 24 hrs by using the Image Analyser system. There was a significant reduction in the size of the calcium oxalate dihydrate crystals by all of the extracts except for petroleum ether extract compared to the control (p < 0.05). The significant reduction in the size of the monohydrate crystals was only observed in the n-butanol and water extracts (p < 0.05). The size of monohydrate crystal reduced significantly in n-butanol and water compared to zylorik (p < 0.05). The number of monohydrate crystals reduced significantly in the ethanol, n-butanol and water extracts compared to the control and in ethanol(also dyhydrate) and water extractcompared to zylorik (p < 0.05). These results indicated that most of the extracts of P. major could reduce the number and size of calcium oxalate crystals in vitro at 10 mg/ml after 24 hrs.