RNA-SEQ NGS DATA ANALYSIS


  •  

  • This workshop will present recent methods of mapping sequence reads generated by an RNA-seq experiment to a reference genome (human genome),assigning reads to genome features (exon, intron, etc), and using the expression levels of those features to identify differentially expressed genes between different conditions (cancer vs normal, treated vs untreated, etc). We will examine some of the methods, their principles and biases in every step. We will look at techniques to quantify our results, and some of the pitfalls to be aware of. At the end of this workshop, participants will have the ability to perform analysis of a realistic/own dataset, from data retrieval through differential gene expression, and have an appreciation of the available data sources and tools, be deeply aware of biases, and be able to use these insights to critically interpret the results.

     

    Objectives

    1. To give participants a combination of lecture and hands-on bioinformatics analysis
    2. To expose and introduce a basic concept of RNA-seq technology
    3. To provide the fundamentals concepts of differential gene expression
    4. To promote awareness of application of RNA-seq in research

  • Facilitators

    Instructors

    • Wan Fahmi Wan Mohamad Nazarie
    • Ang Mia Yang

    Committee

    • Nurul Syakima Ab Mutalib
    • Ryia Illani Mohd Yunos
    • Norshahidah Mahamad Nazir
    • Muhammad Nazirul ‘Azim Mohd Arpandi

  • BANK INFORMATION

    AccountBendahari Universiti Kebangsaan Malaysia
    Account No86-0008114-0
    BankCIMB Berhad
    Bank AddressCIMB Cawangan Taman Maluri, No. 279 & 279A, Jalan Perkasa 1,
    Taman Maluri Off Jalan Cheras, 55100 WP Kuala Kumpur, Malaysia
    CPB/GST UKM0017 8225 9712
    Swift CodeCIBB MYKL

    Account               Bendahari Universiti Kebangsaan Malaysia

    Account No        86-0008114-0

    Bank      CIMB Berhad

    Bank Address    CIMB Cawangan Taman Maluri, No. 279 & 279A, Jalan Perkasa 1,

    Taman Maluri Off Jalan Cheras, 55100 WP Kuala Kumpur, Malaysia

    CPB/GST UKM   0017 8225 9712

    Swift Code          CIBB MYKL

    Secretariat of ‘Introduction to Differential Gene Expression Analysis using RNA-seq’

    Institut Perubatan Molekul UKM (UMBI)

    Jalan Yaacob Latiff, Bandar Tun Razak

    56000 Cheras, Kuala Lumpur, Malaysia

    Fax : +603 – 9171 7185

     

    For any inquiry, please directly contact

    Dr. Wan Fahmi Wan Mohamad Nazarie  (wanfahmi@ukm.edu.my)

    Madam Ryia Illani (ryia.yunos@ppukm.ukm.edu.my)

  • The Bioinformatics Workshop will be held at UKM Medical Molecular Biology Institute (UMBI), Kuala Lumpur that is located just a 15-minute drive away from the heart of Kuala Lumpur city centre.

    For more information about the venue, please visit the UMBI website at http://www.ukm.my/umbi/.

    Please take note that the accommodation is NOT included in the registration fee. Kindly refer to the Accommodation tab for further information.

    WAZE:https://www.waze.com/directions/malaysia/kuala-lumpur/umbi-ukm/66650143.666632502.6392182.html

    GOOGLE MAP:https://goo.gl/maps/soeAgH9K4292

  • Hotel nearby UMBI:

    Hotel Sri Permaisuri Cheras (3-star hotel)

    Hotel EST Kuala Lumpur

    International Youth Centre (IYC) Residence

    Low Budget hotel are also available nearby the UMBI. Please refer to any hotel booking website for further information.

     

    **Important Note: KINDLY BE NOTED THAT THE ORGANIZER WILL NOT BE LIABLE FOR ANY CLAIM OR RESPONSIBILITY IN REGARDS OF THE LISTED ACCOMMODATION ABOVE. FOR ACCOMMODATION BOOKING AND RESERVATION, PLEASE CLICK THE APPROPRIATE LINKS AND FOLLOW THE GUIDELINES IN THE RESPECTIVE WEBSITE.

  • Dr. Wan Fahmi Wan Mohamad Nazarie (wanfahmi@ukm.edu.my) or

    Madam Ryia Illani (ryia.yunos@ppukm.ukm.edu.my)

     

    Institut Perubatan Molekul UKM (UMBI)

    Pusat Perubatan UKM (PPUKM),

    Jalan Yaacob Latiff, Bandar Tun Razak

    56000 Cheras, Kuala Lumpur,

    MALAYSIA

    Fax: +603 – 9171 7185

    WAZE: https://www.waze.com/directions/malaysia/kuala-lumpur/umbi-ukm/66650143.666632502.6392182.html
    GOOGLE MAP: https://goo.gl/maps/soeAgH9K4292