Imaging Resource

Our imaging facility is equipped with the Leica TCS SP5 II confocal microscope, Nikon Eclipse 80i Advanced research digital microscope

Leica TCS SP5 II confocal microscope

The confocal microscope offers several advantages over conventional optical microscopy, including controllable depth of field, the elimination of image degrading out-of –focus information, the ability to collect serial optical sections from thick specimens. The key to the confocal approach is the use of spatial filtering to eliminate out-of-focus light or flare in specimens that are thicker than the plane focus. There has been tremendous explosion in the popularity of confocal microscopy in recent years, due in part to the relative ease with which extremely high-quality images can be obtained from specimens prepared for conventional optical microscopy. There are applications in many areas of research in cell and molecular biology. Our lab is designed with dim lighting to reduce fading of the fluorescence probe during imaging. The confocal microscope also comes with an incubation chamber to allow real-time live imaging of cells for more than 24 hours.

Nikon Eclipse 80i Advanced Research Digital Microscope

This is a widefield epifluorescent microscope that allows quick and easy access to a simple to use but using high quality objective lenses.The Nikon microscope delivers remarkably high signal to noise ratios in producing fluorescence images. Fluorescent-tagged structures or proteins can be located and the cellular morphology of specimens can be visualized using a high-performance DIC method in combination with Hi S/N epi-fluorescence illumination. The microscope is for the visualization tissue/cells fixed on glass slide.