– Cell Culture & Stem Cell Laboratory

This is a state-of-the-art laboratory which houses all the equipments required to perform cell culture and stem cells experiments along with non-radioactive cell-based assays. The lab has well organized workstations, Class II Biosafety cabinets, centrifuges, CO2 incubators, refrigerators, freezers and the latest inverted microscopes.

Both the general cell culture lab and the stem cell lab are used for culturing and maintain live cells from cell lines, primary cells and embryonic stem cells. All cell culture and stem cell users are required to undergo a structured training programme and then evaluated on their cell culture aseptic techniques before being allowed for full access to the lab facilities. The laboratory can be used by researchers outside UKM for a competitive charge.

The list of cell lines available:

STOCK OF CELL LINES IN UMBI 2016
NoCell OriginOrganismCell Line DesignationAttc Catalog NoDiagnosisMedia
Brain
1BrainHomo sapiens, human1321N1Grade II Brain Astrocytoma DMEM
2A172CRL1620Brain GlioblastomaDMEM
3LN18CRL-2610Grade IV glioblastoma, gloimaDMEM
4SHS-Y-5YCRL-2266Brain NeuroblastomaDMEM
5U87MGHTB 14Grade IV glioblastoma, AstrocytomaDMEM
Breast
6BreastHomo sapiens, humanHCC 202CRL 2316TNM stage IIIA, Grade 3, Primary Ductal CarcinomaRPMI-1640
7T-47DHTB-133Ductal CarcinomaRPMI-1640
Colorectal
8ColorectalHomo sapiens, humanSW 480CCL 228Dukes type B, AdenocarcinomaL-15
9SW 48CCL 231Dukes type C, AdenocarcinomaL15
10COLO 320DMCCL 220Dukes type C, AdenocarcinomaRPMI-1640
11COLO 205CCL 222Dukes type D, AdenocarcinomaRPMI-1640
12HCT 116CCL-247AdenocarcinomaMc Coy
13HT 29HTB 38AdenocarcinomaMc Coy
14CCD 112 CoNCRL 1541NormalEMEM
15CCD 841 CoNCRL 1790NormalEMEM
Embryonic Kidney
16Embryonic KidneyHomo sapiens, humanHEK 293CRL 1573hypotriploid human cell line / Contain AdenovirusEMEM
Endometrium
17EndometriumHomo sapiens, humanECC-1CRL2923AdenocarcinomaRPMI-1640
18HEC-1-AHTB-112Mc Coy
19KLECRL 1622DMEM:F12
Liver
20Liver / GallbladderHomo sapiens, humanHLF-aHepatoma non differentiatedDMEM
21LiverWRL 68CL48Normal, Contain HPV
Lungs
22LungsHomo sapiens, humanA549CCL 185CarcinomaF12-K
23NCI-H1299CRL 5803Carcinoma, non-small cell lung cancerRPMI-1640
Ovary
24OvaryHomo sapiens, humanCAOV-03HTB 75AdenocarcinomaDMEM
25SK-OV-3HTB 77Mc Coy
Prostate
26ProstateHomo sapiens, humanDU145HTB 81CarcinomaEMEM
27PC 3CRL 1453Grade IV, AdenocarcinomaF12-K
28RWPE-1CRL-11609NormalK-SFM
WBC
29WBCHomo sapiens, humanMV-4-11CRL-9891AMLIMDM

Updated: 12/05/2016