– Metabolic Laboratory
The metabolic laboratory is mainly supported by The Malaysian Cohort (TMC) project. The lab was established to support various validation studies under TMC. Various methods have been developed and validated including the analysis of urine cotinine, erythrocyte thiopurine methyltransferase enzyme, plasma malondialdehyde and carotenoids.
The laboratory is equipped with the nitrogen evaporator and the microplate readers which cater for different detection modes (absorbance, fluorescence and luminescence), UV-VIS Spectrophotometer, High Performance Liquid Chromatography (HPLC) (Agilent 1200 LC series) and Ultra Performance Liquid Chromatography (UPLC) (Waters ACQUITY UPLC) systems.
The presence of TurboVap Caliper Nitrogen Evaporator allows for fast unattended concentration or evaporation of samples during sample preparation for bioanalysis. Photometric quantitative determination of different analytes such as transition metal ions, highly conjugated organic compounds and biological macromolecules is feasible with the existing UV-VIS spectrophotometer (PG Instrument). In addition, the microplate readers enable micro-volume research application in a high throughput manner. The reader with absorbance detection system (Biotek EL800) allows the quantitation for assays such as ELISA, enzyme activity assays, protein and nucleic acid quantification whereas the fluorescence detection system (Thermo Varioskan Flash Microplate Reader) is used for fluorescently labelled cell-based and biochemical applications. The microplate reader with luminescence detection system (Molecular Devices SpectraMax Luminometer) is used for luciferase-based assays as well as cell viability and cytotoxicity assays.
The core equipments in the Metabolic Laboratory are the HPLC and UPLC systems, both installed with Diode Array (DAD) and Fluorescence (FLR) detectors. The HPLC system offers high resolution and sensitivity by providing full power of multiple wavelength detection and spectral information by DAD and FLR. The UPLC is usually used for its higher throughput and productivity than the HPLC. It is up to nine times faster, has up to twice the peak resolution, and is up to three times more sensitive than traditional HPLC. Both systems have built-in GLP/GMO compliance features including automated wavelength verification, tracking of system performance and maintenance.
Besides being used for our own in-house research projects, we welcome service enquiries from the industry or researchers outside UKM.