UMBI-QIAGEN-REAL-TIME-PCR-WORKSHOP 2019

 

  • INTRODUCTION

    The workshop is designed for users who are new to real time quantitative PCR (qPCR), but all users are welcome. User will experience converting RNA to cDNA, optimizing a qPCR reaction, assessing primers efficiency and determining expression level of their genes of interest. Clients will gain experience using DNA binding dyes chemistries. Lectures will include an overview of gene expression, assay design and optimization, template preparation, analysis of results, additional applications of real-time PCR technology, and many more. Data analysis and simple statistical test will be included. Some previous experience using standard PCR or real time PCR is helpful but not necessary for workshop attendees.

    OBJECTIVES

    Our objectives are:

    • To give participants a combination of lecture and hands-on laboratory experience using qPCR methods
    • To expose and introduce a basic concept of qPCR and the analysis involved
    • To provide the fundamentals of cDNA synthesis, quality controls in qPCR
    • To promote awareness of the applications of qPCR in research
    • To support research in Molecular Medicine, especially for the local scientists

  • Time Day 1 Day 2
    8:00 amRegistration and breakfastBreakfast
    9:00 amLecture 2:
    Introduction to fundamentals of qPCR
    (Seminar room, Level 5)
    Hands-on 3:
    qPCR standard curve analysis
    (MBU, Level 4)
    10:30 amHands-on 1:
    cDNA synthesis
    (MBU, Level 4)
    Hands-on 4:
    Performing relative quantification qPCR on Biorad CFX96
    (MBU, Level 4)
    12:30 - 1:00 pmUMBI lab visits and photo sessions
    1:00 pmLunch
    (Level 1)
    Lunch
    (Level 1)
    2:30 pmHands-on 2:
    qPCR primers optimization (gradient qPCR)
    (MBU, Level 4)
    Hands-on 5:
    Data analysis of Relative quantification qPCR
    (Bioinformatics lab, Level 3)
    3:30 pmLecture 2:
    Applications of qPCR in research - UMBI’s experience
    (Bioinformatics lab, Level 3)
    4:00 pmDay 1 Review and TeaDay 2 Review and Tea

  •  

    Institut Perubatan Molekul UKM (UMBI)
    Jalan Yaacob Latiff, Bandar Tun Razak
    56000 Cheras, Kuala Lumpur, Malaysia
    Fax : +603 – 9171 7185

    For any inquiry, please directly contact Dr. Nurul Syakima (syakima@ppukm.ukm.edu.my) , Madam Ryia (ryia.yunos@ppukm.ukm.edu.my)

  • The UMBI-QIAGEN REAL TIME PCR WORKSHOP – GENE EXPRESSION ANALYSIS will be held at UKM Medical Molecular Biology Institute (UMBI), Kuala Lumpur that is located just a 15-minute drive away from the heart of Kuala Lumpur city center.

    For more information about the venue, please visit the UMBI website at http://www.ukm.my/umbi/.

    Please take note that the accommodation is NOT provided. Kindly refer to the Accommodation tab for further information.

     

    WAZE: https://www.waze.com/directions/malaysia/kuala-lumpur/umbi-ukm/66650143.666632502.6392182.html

    GOOGLE MAP: https://goo.gl/maps/soeAgH9K4292

  • Hotels nearby UMBI:

    1. Hotel Sri Permaisuri Cheras (3-star hotel)
    2. Hotel EST Kuala Lumpur
    3. International Youth Centre (IYC) Residence

    Low Budget hotel are also available nearby the UMBI. Please refer to any hotel booking website for further information.

    **Important Note : KINDLY BE NOTED THAT THE ORGANIZER WILL NOT BE LIABLE FOR ANY CLAIM OR RESPONSIBILITY IN REGARDS OF THE LISTED ACCOMMODATION ABOVE. FOR ACCOMMODATION BOOKING AND RESERVATION, PLEASE CLICK THE APPROPRIATE LINKS AND FOLLOW THE GUIDELINES IN THE RESPECTIVE WEBSITE.

  • Dr. Nurul Syakima (syakima@ppukm.ukm.edu.my), Madam Ryia (ryia.yunos@ppukm.ukm.edu.my)
    Institut Perubatan Molekul UKM (UMBI)
    Jalan Yaacob Latiff, Bandar Tun Razak
    56000 Cheras, Kuala Lumpur, Malaysia
    Fax : +603 – 9171 7185