Liquid Chromatography Mass Spectrometer (LC-MS)

Brand : Dionex(LC),Bruker(MS)
Model : MicroTOF-Q
Range : 50 – 3000 m/z with resolution of 15000 FWHM

Liquid chromatography–mass spectrometry (LC-MS) is an analytical chemistry technique that combines the physical separation capability of liquid chromatography (or HPLC) with the mass analysis capability of mass spectrometry (MS). Coupled chromatography – MS systems are popular in chemical analysis because the individual capability of each technique is enhanced synergistically. While liquid chromatography separates mixtures with multiple components, mass spectrometry provides structural identity of the individual components with high molecular specificity and detection sensitivity. This tandem technique can be used to analyse both positively and negatively ionization technique by turning the components to either protonated or deprotonated compound.

In addition to the liquid chromatography and mass spectrometry devices, an LC-MS system contains an interface that efficiently transfers the separated components from the LC column into the MS ion source. The interface is necessary because the LC and MS devices are fundamentally incompatible. While the mobile phase in a LC system is a pressurized liquid, the MS analysers commonly operate under vacuum (around 10−6 torr).

Thus, it is not possible to directly pump the eluate from the LC column into the MS source unless the flowrate of the column is appropriate for MS source. Overall, the interface is a mechanically simple part of the LC-MS system that transfers the maximum amount of analyte, removes a significant portion of the mobile phase used in LC and preserves the chemical identity of the chromatography products (chemically inert). As a requirement, the interface should not interfere with the ionizing efficiency and vacuum conditions of the MS system. Nowadays, most extensively applied LC-MS interfaces are based on atmospheric pressure ionization (API) strategies like electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI), and atmospheric pressure photo-ionization (APPI).


Range : 50 - 3000 m/z with resolution of 15000 FWHM Applications LCMS is applicable for both volatile and non-volatile samples. It is commonly used to analyse biochemical, organic, and inorganic compounds commonly found in complex samples of environmental and biological origin. Therefore, LC-MS may be applied in a wide range of sectors including biotechnology, environment monitoring, food processing, and pharmaceutical, agrochemical, and cosmetic industries.
• Environmental monitoring
• Food, beverage, flavour and fragrance analysis
• Forensic and criminal cases
• Biological and pesticides detections
• Astro chemistry and geo chemical research
• Medicine and pharmaceutical applications
• Petrochemical and hydrocarbons analysis
• Clinical toxicology
• Academic research
• Industrial applications
Sample Requirements
• Liquid - organic solvents (MeOH, ACN)
• High purity water
• Chloroform

Additional information


B.Sc. (UKM) Biochemistry


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03-8911 8506