Workshop Outline

Gas chromatography coupled to mass spectrometry (GC-MS) is an essential instrument for high-throughput metabolomics studies especially for VOCs profiling. GC-MS utilizes a quadrupole mass spectrometer capable of high-speed scanning, which enables reliable and fast metabolites identification that are biologically relevant. Generally, samples are normally derivatized prior to GC-MS analysis in order to reduce their polarity and facilitate chromatographic separation. The electron ionization (EI) mass spectra obtained are reproducible and suitable for library matching, mass spectral collections being readily available.

In this 3-day workshop, we will provide a detailed practical GC-MS based metabolomics that covers sample preparation (extraction and derivative methods of GC-MS, with hands-on), data acquisition including qualitative and quantitative analysis and finally data analysis using univariate and multivariate approaches.

Who should attend

Those who are interested in metabolomics and its applications (research scientists, academicians, postdoctoral fellows, and students) are encouraged to attend the workshop.

List of Speakers and Trainers

  • ChM. Dr. Kamalrul Azlan Azizan is a research fellow at INBIOSIS, UKM. He completed his PhD in Systems Biology in 2014 at the Universiti Kebangsaan Malaysia (UKM) in 2014. His research interest includes plant and microbial metabolomics as well as the application of chemometrics and multivariate for biomarkers discovery. Currently, he is working on bioprospecting allelochemicals from Wedelia trilobata using chemometrics and predictive models. 

  • ChM. Dr. Kamalrul Azlan Azizan has a strong hands-on experience with GC-MS and LC-MS systems and actively involves in the development and optimization of GC/LC methods, giving guidance through application and troubleshooting assistance. He can be contacted via email; kamalrulazlan@ukm.edu.my 

  • Dr. Ahmed Mediani obtained his PhD in Food Science from Universiti Putra Malaysia in 2016. He then served as a Postdoctoral Fellow at the Atta-ur-Rahman Institute for Natural Product Discovery (AuRIns), UiTM. In 2020, he joined INBIOSIS as a research fellow. 

  • His research focuses on the application of metabolomics in natural products and food Science. He has vast experiences in in vivo and in vitro assays on natural products as well as NMR- and LCMS-based metabolomics analyses. As a researcher, He is actively involved in research projects as well as preparing research proposal, particularly in the area of metabolomics, natural product chemistry and food Science. His recent project entitled NMR-based metabolomics approach revealing metabolic signatures and mechanism associated with anti-diabetic effects of Annona muricata

  • Please feel free to visit his research group website or contact Dr. Ahmed Mediani (ahmed@ukm.edu.my) for research and industrial projects collaboration.

Programme

29th March 2022 (Tuesday)

Time Activity Venue Trainer
8.30 am Registration Foyer -
9.00 am Opening ceremony and introduction Seminar Hall -
9.10 am Lecture 1 : Introduction to GC-MS Metabolomics Seminar Hall Dr. Ahmed Mediani
10.30 am Tea break Foyer -
11.00 am Lecture 2 : Fundamental of Sample Preparation Seminar Hall Dr. K. Azlan
12.30 pm Lunch break Foyer -
2.00 pm Practical 1 : Sample Preparation Hands-on Metabolomics Lab Dr. K. Azlan & Mr.  Syahmi Afiq Mustaza
4.o0 pm Afternoon break Foyer -
4.15 pm Summary, discussions and wrap-up Seminar Hall Dr. Ahmed Mediani & Dr. K. Azlan
5.00 pm END OF DAY 1

30th March 2022 (Wednesday)

Time Activity Venue Trainer
9.00 am Introduction to Day 2 Seminar Hall -
9.10 am Lecture 3 : Fundamental of GC-MS and derivatization Metabolomics Lab Dr. K. Azlan & Mr.  Syahmi Afiq Mustaza
10.30 am Tea break Foyer -
11.00 am Practical 2 : Sample Derivatization Metabolomics Lab Dr. K. Azlan & Mr.  Syahmi Afiq Mustaza
12.30 pm Lunch break Foyer -
2.00 pm Practical 3 : Software Troubleshooting Metabolomics Lab Dr. K. Azlan & Mr.  Syahmi Afiq Mustaza
4.o0 pm Afternoon break Foyer -
4.15 pm Summary, discussions and wrap-up Seminar Hall  Dr. K. Azlan
5.00 pm END OF DAY 2

31st March 2022 (Thursday)

Time Activity Venue Trainer
9.00 am Introduction to Day 3 Seminar Hall -
9.10 am Lecture 4 : Fundamental of Data Pre-processing Seminar Hall Dr. K. Azlan
10.30 am Tea break Foyer -
11.00 am Practical 5 : Data Analysis Seminar Hall Dr. Ahmed Mediani & Dr. K. Azlan
12.30 pm Lunch break Foyer -
2.00 pm Practical 6 : Data Visualization and Multivariate Analysis Seminar Hall Dr. Ahmed Mediani
4.o0 pm Afternoon break Foyer -
4.15 pm
  • Summary, discussions and wrap-up
  • Presentation of attendance certificate
Seminar Hall Dr. Ahmed Mediani & Dr. K. Azlan
5.00 pm END OF DAY 3

Register now or contact us for more details