Institute of Systems Biology

 Institute of Systems Biology

Workshop Outline

This workshop will focus on the scientific method of Recombinant Protein Purification and Enzyme Characterization through theory and hands-on. In addition, protein purity and homogeneity analysis, in silico structure prediction, and structure functional analysis will be discussed.

UPON THE COMPLETION OF THIS WORKSHOP, PARTICIPANTS WILL BE CAPABLE OF:
• Recombinant protein purification from E.coli expression system
• Protein purity and homogeneity analysis
• Enzyme characterization
• Protein structure prediction and analysis

Who should attend

Postgraduate students (Master and PhD students), Post Doctorate, Academicians and Researchers (Government and non-government institution)

List of Speakers and Trainers

  • Associate Professor Dr. Ng Chyan Leong is a structural biologist. He completed his PhD in York Structural Biology Laboratory (YSBL), Chemistry Department, University of York, UK in 2007 under the supervision of Prof. Dr. Fred Antson. He then worked as a Postdoctoral Fellow in Dr. Venki Ramakrishnan’s group at the Medical Research Council, Laboratory of Molecular Biology (MRC-LMB), Cambridge, UK, focusing on ribosome and protein translation. He joined INBIOSIS in 2012 as a Research Fellow and has established the recombinant protein purification, protein crystallization and protein analysis platform in the Institute.

  • Assoc. Prof. Dr. Ng Chyan Leong’s research interests are to unveil the structure and function of conserved hypothetical proteins, secondary metabolites biosynthesis enzymes, toxin and epitope of allergen molecules. His group determines the atomic resolution protein structure using X-ray crystallography, and applies biochemistry, biophysics, molecular biology and bioinformatics for structural and functional analysis. Together with national and international collaborators, his group has determined the atomic resolution crystal structure of the human CZIB protein, house dust mite allergen, Hydra Actinoporin-Like Toxin (HALT), nerol dehydrogenase and thermophilic amylase, and characterized several secondary metabolites biosynthesis enzymes and proteins with unknown function. Dr. Ng’s research interest is also extended to understanding the impact of carbon sources (glucose vs triglyceride) in fungi metabolite biosynthesis, and plant alkaloid biosynthesis pathway using systems biology approaches, which may lead to the development of alternative microbial fermentation platform and drug discovery. His group has received various university and national research grants, and actively collaborates with industry partners. Their research output has been published in peer-reviewed indexed journals (https://orcid.org/0000-0001-8590-7418) including Nature Communications, Scientific Reports, Plant Physiology and Biochemistry, Phytochemistry, Peer J and Microbial Cell Factories.

  • Please feel free to contact Dr. Ng (clng@ukm.edu.my) for research and industrial projects collaboration. Research students are welcomed to join the group. 

  • Associate. Prof. Dr. Maizom Hassan obtained her Bachelor (1999), Masters (2004) and Ph.D. Degrees (2008) in Bioresources Science from the University of Tottori, Japan. After completing her studies, she was appointed as a Research Fellow/Senior Lecturer at the Institute of Systems Biology (INBIOSIS), Universiti Kebangsaan Malaysia (UKM). Her current research focuses on the development of biorational insecticide for oil palm pest management by using integrated systems biology, enzymology and entomology approaches. Her other research interests are purification and characterization of several microbial and plant enzymes. Her research interests were manifested in 2 patents, 1 book, 1 book-chapter and 29 publications which include 22 publications in high-impact journals such as Plants, Plant Physiology and Biochemistry, Insects, Phytochemistry, Genes and PloS ONE. Her publications attracted 153 citations with h-index of 8.

  • Associate. Prof. Dr. Maizom has been instrumental in establishing industry collaboration with Sime Darby, the largest listed palm oil company globally, Felda Global Ventures R&D Sdn. Bhd, Malaysia Cocoa Board and Department of Agriculture Malaysia. She managed to secure around RM6.6 million of cumulative research funding, of which RM1.4 million as the principal investigator. Currently, she oversees 7 Ph.D., 1 MSc, and 2 BSc students as a Main Supervisor. Dr. Maizom assumed the role as referees for articles in peer-reviewed journals such as GM Crops & Food-Biotechnology in Agriculture and the Food Chain, Journal of Oil Palm Research, Sains Malaysiana and Journal of Tropical Plant Physiology. She was also appointed as expert panel/evaluator for Research Foundation Flanders (FWO) Post-Doctoral Fellowship under the European Science Foundation (ESF).

  • Expressions of interest in joining the research collaboration and activity with Associate. Prof. Dr. Maizom’s lab are always welcome. Please contact her via e-mail (maizom@ukm.edu.my) for further discussion.

Programme

Day 1: 14th December 2022 (Wednesday)

Time Activity Venue
0845 – 0915 Breakfast and Registration Foyer
0915 – 1030  Lecture 1: Introduction to

  • Recombinant protein expression systems
  • Protein Purification 
    • Cell lysis
    • Affinity Chromatography
    • Size Exclusion Chromatography
    • Fast protein liquid chromatography (FPLC) 
Seminar Hall
1030 – 1045 Tea break   Foyer
1045 – 1230 Lecture 2: Introduction to

  • Analysis of protein purity and homogeneity
  • SDS-PAGE, Native PAGE, SEC, Mass-spec and DLS 
Seminar Hall
1230 – 1400  Lunch  Foyer
1400 – 1630  Hands-on session 1:  Protein purification (sample preparation and FPLC analysis) Proteomics Lab
1630 – 1700  Tea break / end of the session Foyer

Day 2: 15th December 2022 (Thursday)

Time Activity Venue
0845 – 0900  Breakfast Foyer
0900 – 1030  Lecture 3: In silico structure prediction and functional analysis of protein Seminar Hall
1030 – 1045 Tea Break  Foyer
1045 – 1230 Lecture 4: Introduction to

  • Protein biochemical characterization
  • Enzyme assay
  • Substrate specificity 
Seminar Hall
1230 – 1400 Lunch Foyer
1400 – 1530 Hands-on session 2: Protein characterization

  • Standard Enzyme assay
  • Effects of pH and temperature on enzyme activity
  • Substrate specificity
Proteomics Lab
1600 – 1630  Discussion, summary and wrap-ups Seminar Hall
1630 – 1700  Tea-time & end of session Foyer

Register now or contact us for more details