Institut Biologi Sistem

 Institute of Systems Biology

Workshop Outline

This workshop will focus on the introduction to proteomics analysis through theory and hands-on.

Who should attend

Postgraduate students (Master and PhD students), Post Doctorate, Academicians and Researchers (Government and non-government institution)

List of Speakers and Trainers

  • Associate. Prof. Dr. Maizom Hassan obtained her Bachelor (1999), Masters (2004) and Ph.D. Degrees (2008) in Bioresources Science from the University of Tottori, Japan. After completing her studies, she was appointed as a Research Fellow/Senior Lecturer at the Institute of Systems Biology (INBIOSIS), Universiti Kebangsaan Malaysia (UKM). Her current research focuses on the development of biorational insecticide for oil palm pest management by using integrated systems biology, enzymology and entomology approaches. Her other research interests are purification and characterization of several microbial and plant enzymes. Her research interests were manifested in 2 patents, 1 book, 1 book-chapter and 29 publications which include 22 publications in high-impact journals such as Plants, Plant Physiology and Biochemistry, Insects, Phytochemistry, Genes and PloS ONE. Her publications attracted 153 citations with h-index of 8. Expressions of interest in joining the research collaboration and activity with Associate. Prof. Dr. Maizom’s lab are always welcome. Please contact her via e-mail (maizom@ukm.edu.my) for further discussion.

  • Dr. Hamizah Shahirah Hamezah's research group has a great interest in understanding the mechanisms of learning and memory, as well as interventions for the cognitive decline during aging and neurodegenerative diseases. They focus on finding possible biomarkers leading to the cognitive decline in brain aging and neurodegenerative diseases by using the state-of-art omics approaches, brain imaging, and cognitive neurosciences. They are interested in finding possible therapeutics for the treatment of neurodegenerative diseases by investigating the effect of natural compounds on transgenic animal models. If you are interested in collaborating with her, please email her at hamizahshahirah@ukm.edu.my.

Programme

8th October 2024 (Tuesday)

Time Activity Speaker Venue
8.30 am Registration Foyer
9.00 am Introduction Assoc. Prof. Dr. Maizom Hassan Seminar Hall
9.05 am Lecture 1: Introduction to Proteomics - Part 1
● What is proteomics?

● Gel-based & non gel-based proteomics

Assoc. Prof. Dr. Maizom Hassan Seminar Hall
10.30 am Morning break Foyer
11.00 am Lecture 2: Introduction to Proteomics - Part 2
● Protein extraction

● Protein measurement

● Protein visualization

Assoc. Prof. Dr. Maizom Hassan Seminar Hall
1.00 pm Lunch break
2.00 pm  Practical 1:
● Protein extraction
● Protein measurement
● SDS-PAGE electrophoresis
● Protein visualization
●Assoc. Prof. Dr. Maizom Hassan
● Dr. Hamizah Shahirah Hamezah
● AJK kursus latihan
Proteomics Lab
5.00 pm Tea break / END OF DAY 1   Foyer

 

9th October 2024 (Wednesday)

Time Activity Speaker Venue
9.00 am Introduction Dr. Hamizah Shahirah Hamezah Seminar Hall
9.30 am Lecture 3: Introduction to Proteomics - Part 3
● Mass spectrometry analysis in proteomics
Dr. Hamizah Shahirah Hamezah Seminar Hall
10.30 am Morning break
11.00 am Practical 2:
● In-gel digestion tutorial and quality checking
●Assoc. Prof. Dr. Maizom Hassan
● Dr. Hamizah Shahirah Hamezah
● AJK kursus latihan
Proteomics Lab
1.00 pm Lunch break
2.00 pm Practical 3:
● LC-MS-TOF demonstration
● Dr. Hamizah Shahirah Hamezah
● AJK kursus latihan
Chemical Analysis Workshop
3.00 pm Lecture 4: Introduction to Proteomics-Part 4
● Understanding mass spectra
Dr. Hamizah Shahirah Hamezah Seminar Hall
5.00 pm Tea-time & end of session Foyer

10th October 2024 (Thursday)

Time Activity Speaker Venue
9.00 am Introduction Dr. Hamizah Shahirah Hamezah Seminar Hall
9.05 am Lecture 5:
● Introduction to MaxQuant for proteomics data analysis
Dr. Hamizah Shahirah Hamezah Seminar Hall
10.30 am Morning break
11.00 am Practical 4:
● MaxQuant for proteomics data analysis
● Dr. Hamizah Shahirah Hamezah
● AJK kursus latihan
Seminar Hall
1.00 pm Lunch    
2.00 pm Lecture 6:
● Introduction to Perseus for proteomics data analysis
Dr. Hamizah Shahirah Hamezah Seminar Hall
3.00 pm Practical 5:
● Perseus for statistical analysis of proteomics data
Dr. Hamizah Shahirah Hamezah Seminar Hall
4.30 pm Closing ceremony ●Assoc. Prof. Dr. Maizom Hassan
● Dr. Hamizah Shahirah Hamezah
● AJK kursus latihan
Seminar Hall
5.00 pm Tea time / End of workshop Foyer

 

Register now or contact us for more details