Workshop Outline

This workshop is aimed at giving participants a detailed understanding of real-time quantitative PCR: 

  • qPCR primer design and experimental considerations
  • Considerations in conducting standard curve and gene expression experiment
  • Hands-on analysis of qPCR data
  • Different applications of qPCR

For just RM 100 (USD 25), join our guided hands-on program that will be personal yet instructive covering topics from the top-to-toe basics of detection chemistry to the technicalities of the real-time PCR workflow.

We will be using MS Excel for this workshop.

Who should attend?

​This workshop is open to local & international participants, from beginner to advanced researchers in the field.

Speaker Biography

  • Associate Professor Dr. Goh Hoe Han is a plant molecular biologist who obtained his PhD from the University of Sheffield, United Kingdom in 2011 before starting his first acedemic position at the Institute of Systems Biology, Universiti Kebangsaan Malaysia. Upon joining INBIOSIS, he pioneered the Plant Functional Genomics Research Group focusing on the molecular exploration of tropical plants and crop improvement using functional genomics approaches. Some of the tropical plant species include kesum, ketum, Rafflesia, and Nepenthes pitcher plants, as well as important crops, such as mangosteen, papaya, rice, tomato, and oil palm. Fundamental understandings on the molecular aspects of these plants are crucial for their bioprospecting, industrial application, and crop improvement.

  • Dr Goh is keen on industrial collaboration in precision biotechnology for tropical plant improvement and also the commercialisation of novel recombinant plant enzymes. If you are interested to collaborate or be part of the plant functional genomics group, please email to gohhh@ukm.edu.my.

Programme

13th July 2022 (Wednesday)

Time Activity
9.00 am Registration
9.30 am Introduction to qPCR

  • Technical qPCR discussion (Terminology & Assay characteristics)
  • Introduction to MIQE Guidelines
  • Detection Chemistry: DNA binding dyes and hydrolysis probe
  • Primer and Probe Design
  • How does a real-time qPCR system work?
10.45 am Tea break
11.00 am Considerations of qPCR experiment
12.00 pm Experimental design for gene expression study
12.30 pm Technical Talk 
1.00 pm Lunch break
2.00 pm Hands-on practical: qPCR Data Analysis using an Excel Template
3.30 pm Tea break
3.45 pm Applications of qPCR
4.30 pm Questions, Discussion & Closing
5.00 pm End of Workshop

 

Register now or contact us for more details