Sains Malaysiana 42(5)(2013): 625–628


In Vitro Antidiabetic Activity of Nisamalaki Churna

(Aktiviti Antidiabetik In Vitro Nisamalaki Churna)



B.S. Ashok Kumar* & N.K. Manjunath

Department of Pharmacognosy, Sri K.V. College of Pharmacy, Chickballapur, Karnataka, India


Saleemulla Khan

Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal, Karnataka, India


Gopi Setty Saran

Department of Pharmaceutics, Sri K.V.College of Pharmacy, Chickballapur, Karnataka, India


R. Nandeesh

Department of Pharmacognosy, SreeSiddaganga College of Pharmacy, Tumkur, Karnataka, India


Received: 27 March 2012/Accepted: 2 October 2012



Nisamalaki Churna is an Ayurveda formulation used for diabetes, which consists of amalaki and haridra. In vitro antidiabetic screening of Nisamalaki Churna by α-amylase inhibition was carried out by starch iodine method, initrosalicylic acid method (DNSA) and effect on normal rats blood sugar level was studied. Nisamalaki Churna shows potent α-amylase inhibition IC50 89.44 μg/mL by starch iodine method and IC50100.0 μg/mL by DNS method. Nisamalaki Churna showed potent decrease in blood glucose level in normal rats.


Keywords: α-amylase inhibition; antidiabetic screening; Nisamalaki Churna



Nisamalaki Churna adalah formula Ayurveda untuk diabetis yang mengandungi amalaki dan haridra. Penyaringan antidiabetis in vitro Nisamalaki Churna telah dijalankan. Kesan Nisamalaki Churna terhadap perencatan α-amilase telah dikaji menggunakan kaedah iodin kanji. Dengan menggunakan kaedah asid dinitrosalisilik (DNSA) kesan terhadap aras gula dalam darah tikus biasa telah dijalankan. Nisamalaki Churna menunjukkan kesan perencatan alfa amilase yang kuat, IC50 89.44 μg/mL melalui kaedah iodin kanji dan IC50100.0 μg/mL DNS. Nisamalaki Churna menunjukkan penurunan aras glukos yang kuat dalam tikus biasa.


Kata kunci: Nisamalaki Churna; penyaringan antidiabetis; perencatan α-amylase


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