Sains Malaysiana 44(7)(2015): 947–955

Pure Cultivation and Morphological Studies of Four Chaetoceros Taxa from the Coastal Waters of Pahang, Malaysia

(Pengkulturan Tulen dan Kajian Morfologi Empat Takson Chaetoceros dari Persisiran Pantai Pahang, Malaysia)

 

M.A.M. FUAD1*, N. MOHAMMAD-NOOR2, A.K.C. JALAL2 & B.Y. KAMARUZZAMAN2

 

1Institut Kesihatan Umum, Kementerian Kesihatan Malaysia, Jalan Bangsar, 50590 Kuala Lumpur

Malaysia

 

2Kulliyyah of Science, International Islamic University Malaysia, Jalan Istana, Bandar Indera Mahkota, 25200 Kuantan, Pahang Darul Makmur, Malaysia

 

Received: 1 December 2013/Accepted: 2 February 2015

 

ABSTRACT

This study was conducted to study the morphological features of species under the genus Chaetoceros isolated from the coastal waters of Pahang, Malaysia. The species were isolated, cultivated and viewed under light microscope (LM) and scanning electron microscope (SEM). Four taxa were successfully isolated and cultivated in pure culture, namely Chaetoceros affinis var. affinis, Chaetoceros affinis var. willei, Chaetoceros anastomosans and Chaetoceros baculites. Both varieties of C. affinis were considered as distinct taxa where both strains can be distinguished based on morphological characteristics. C. affinis var. affinis has a thick cell wall compared with C. affinis var. willei. C. anastomosans has special features including a silica bar on the intersection of setae and the production of mucous. The length of the aperture opening is a new record from this study. The new record obtained for C. baculites includes the size of the aperture, the terminal setae, spinal arrangement, the thinness of the cell wall and the location of the species in tropical waters. Detailed data on each species will be added to the taxonomic information for future studies.

 

Keywords: Chaetoceros; coastal water; LM; morphology; SEM

 

ABSTRAK

Kajian ini dijalankan untuk mengkaji ciri morfologi spesies di bawah genus Chaetoceros yang diasingkan dari pesisir pantai Pahang, Malaysia. Kesemua spesies dipencil, dikultur dan diperiksa melalui miskroskop cahaya (LM) dan mikroskop elektron imbasan (SEM). Empat takson telah berjaya dipencilkan dan dikulturkan kepada kultur tulen, iaitu Chaetoceros affinis var. affinis, Chaetoceros affinis var. willei, Chaetoceros anastomosans, dan Chaetoceros baculites. Kedua-dua varieti C. affinis dikategorikan sebagai takson terdekat dengan kedua-dua strain boleh dibezakan berdasarkan ciri morfologi. C. affinis var. affinis mempunyai dinding sel yang tebal berbanding C. affinis var. willei. C. anastomosans mempunyai ciri-ciri istimewa iaitu bar silika di persilangan seta dan penghasilan mukus. Panjang bukaan merupakan rekod terbaru di dalam kajian ini. Rekod terbaru diperoleh bagi C. baculites yang merangkumi saiz bukaan, seta utama, susunan duri dan kenipisan dinding sel dan spesies ini dijumpai di kawasan tropika. Keperincian data untuk setiap spesies akan ditambah ke dalam informasi taksonomi untuk kajian masa hadapan.

 

Kata kunci: Chaetoceros; LM; morfologi; pesisiran pantai; SEM

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 *Corresponding author; email: crystal_fuad@yahoo.com.sg

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