Sains Malaysiana 45(10)(2016): 1435–1442

 

Phytochemical and Biological Evaluation of Defatted Seeds of Jatropha curcas

(Penilaian Fitokimia dan Biologi Biji Jatropha curcas Nyahlemak)

 

MUHAMMAD NISAR UL HAQ1, SULTAN MEHMOOD WAZIR1, FAIZAN ULLAH1*, RAHMAT ALI KHAN2, MIR SADIQ SHAH2 & ADNAN KHATAK1

 

1Department of Botany, University of Science and Technology, Bannu KP, Pakistan

 

2Department of Biotechnology, University of Science and Technology, Bannu KP, Pakistan

 

Received: 14 November 2015/Accepted: 17 February 2016

 

ABSTRACT

In this study, the antimicrobial, antioxidant, phytotoxic and phytochemical properties of defatted seeds of Jatropha curcas were evaluated. A crude methanolic extract of defatted seeds was tested against three fungal strains - Aspergillus niger, Aspergillus flavus and Aspergillus fumigatus - and five bacteria: Escherichia coli and Klebsiella pneumoniae (Gram negative) and Micrococcus luteus, Bacillus subtilis and Staphylococcus aureus (Gram positive). The methanolic extract was diluted in dimethylsulfoxide to final concentrations of 1, 2, 3, 4 and 5 mg/10 mL. The largest zones of inhibition against K. pneumoniae, M. luteus and B. subtilis were achieved using the concentration of 5 mg/10 mL. The concentration of 1 mg/10 mL was most effective against S. aureus and E. coli. In a 1, 1-diphenyl-2-picrylahydrazyl (DPPH) radical scavenging assay, the 5 mg/10 mL concentration of the Jatropha seed extract showed the strongest activity. Higher concentrations of the Jatropha seed extract (10 mg/50 mL and 5 mg/50 mL) significantly inhibited the germination of radish seeds and had negative effects on radish seedling relative water content, shoot length, root length, seedling fresh weight and seedling dry weight (p<0.05). Phytochemical analyses of the defatted seeds detected alkaloids (7.3%), flavonoids (0.39%) and soluble phenolics (mg gallic acid equivalents/g extract). Based on these results, it was inferred that J. curcas seeds contain active ingredients that are effective against pathogenic microbes and therefore could be used to formulate drugs to treat various diseases.

 

Keywords: Antimicrobial; antioxidant; Jatropha curcas; phytochemical; phytotoxicity

 

ABSTRAK

Dalam kajian ini, sifat antimikrob, antioksidan, fitotoksik dan fitokimia biji Jatropha curcas nyahlemak telah dinilai. Ekstrak mentah metanol biji nyahlemak diuji terhadap tiga strain kulat - Aspergillus niger, Aspergillus flavus dan Aspergillus fumigatus dan lima bakteria: Escherichia coli dan Klebsiella pneumoniae (Gram negatif) serta Micrococcus luteus, Bacillus subtilis dan Staphylococcus aureus (Gram positif). Ekstrak metanol dicairkan dalam dimetilsulfoksida hingga ke kepekatan akhir 1, 2, 3, 4 dan 5 mg/10 mL. Zon terbesar perencatan terhadap K. pneumoniae, M. luteus dan B. subtilis telah dicapai menggunakan kepekatan 5 mg/10 mL. Kepekatan 1 mg/10 mL adalah paling berkesan terhadap S. aureus dan E. coli. Dalam 1, 1-diphenyl-2-picrylahydrazyl (DPPH) asai skaveng radikal, kepekatan 5 mg/10 mL ekstrak biji Jatropha menunjukkan aktiviti yang kuat. Kepekatan tinggi ekstrak biji Jatropha (10 mg/50 mL dan 5 mg/50 mL) dengan ketara menghalang percambahan biji lobak dan mempunyai kesan negatif ke atas kandungan air relatif biji lobak, panjang pucuk, panjang akar, berat segar biji dan berat kering biji (p< 0.05). Analisis fitokimia benih nyahlemak dikesan mengandungi alkaloid (7.3%), flavonoid (0.39%) dan fenol larut (mg asid galik setara/ ekstrak gram). Berdasarkan keputusan ini, dapat disimpulkan bahawa biji J. curcas mengandungi bahan aktif yang berkesan terhadap mikrob patogen dan boleh digunakan untuk menghasilkan ubat-ubatan untuk merawat pelbagai penyakit.

 

Kata kunci: Antimikrob; antioksidan; fitoketoksikan; fitokimia; Jatropha curcas

REFERENCES

Adam, K.K. & Liu, R.H. 2002. Antioxidant of activity of grain. Journal of Agriculture and Food Chemistry 50: 6182-6187.

Ademiluyi, B.O. 2013. Evaluation of weed management strategies in cocoyam (Colocosia esculentus L.) production in Ado-Ekiti, Nigeria. International Journal of Agriculture and Soil Sciences 3(2): 38-42.

Arzu, U.T. & Camper, N.D. 2002. Biological activity of common mullein, a medicinal plant. J. Ethnopharmacol. 82: 117-125.

Atta-ur-Rehman. 1991. Studies in Natural Product Chemistry. Netherlands: Elsevier Sci. Publishers. 9: 383-384.

Barkosky, R.R., Einhellig, F.A. & Butler, J.L. 2000. Caffeic acid-induced changes in plant-water relationships and photosynthesis and photosynthesis in leafy spurge (Euphorbia esula L.). J. Chem. Ecol. 26(9): 2095-2109.

Belewu, M.A. 2008. Replacement of fungus treated Jatropha kernel meal for soybean meal in the diet of rats. Green Farming 2(3): 154-157.

Bocco, A., Marie, E.C., Hubert, R. & Claudette, B. 1998. Antioxidant activity and phenolic composition of citrus peel and seed extracts. J. Agric. Food Chem. 46(6): 2123-2129.

Brand-Williams, W., Cuvelier, M.E. & Berset, C. 1995. Use of free radical method to evaluate antioxidant activity. Lebensm Wiss Technology 28: 25-30.

Choudhary, M.I., Dur-e-Shahwar, Perveen, Z., Jabbar, A., Ali, I. & Atta-ur-Rahman. 1995. Antifungal steroidal lactones from Withania coagulance. Phytochem. 40(4): 1243-1246.

Collins, C.H., Lyre, P.M. & Grange, J.M. 1989. Microbiological Methods. 6th end. London: Butterworth-Heinemann.

Edeoga, H.O. & Eriata, D.O. 2001. Alkaloid, tannin and sapnin contents of some medicinal plants. Journal of Medicinal Aromatic Plant Science 23: 344-349.

Ejelonu, B.C., Oderinde, R.A. & Balogun, S.A. 2010. Chemical and biological properties of Jatropha curcas and Mucuna solan seed and seed oil. Libyan Agriculture Research Center Journal International 1(4): 263-268.

Gupta, S.S. 1994. Prospects and perspectives of natural plant products in medicine. Indian Journal of Pharmacology 26: 1-12.

Hayat, M.Q., Khan, M.A., Ashraf, M. & Jabeen, S. 2009. Ethnobotany of the genus Artemisia L. (Asteraceae) in Pakistan. Ethnobotany Research and Applications 7: 147-162.

Idris, A., Al-tahir, I. & Idris, E. 2013. Antibacterial activity of endophytic fungi extracts from the medicinal plant Kigelia africana. Egypt. Acad. J. Biolog. Sci. 5(1): 1-9.

Igbinosa, O.O., Igbinosa, E.O. & Aiyegoro, O.A. 2009. Antimicrobial activity and phytochemical screening of stem bark extracts from Jatropha curcas (Linn). African Journal of Pharmacy and Pharmacology 3(2): 058-062.

James, O., Unekwojo, E.G. & Ojochenemi, A.A. 2011. Assessment of biological activities: A comparison of Pergularia daemia and Jatropha curcas leaf extracts. British Biotechnology Journal 1(3): 85-100.

Khan, A.M., Qureshi, A.M., Gillani, S.A. & Ullah, F. 2011a. Antimicrobial activity of selected medicinal plants of Margalla Hills, Islamabad, Pakistan. Journal of Medicinal Plants Research 5(18): 4665-4670.

Khan, A.M., Qureshi, R.A., Ullah, F. & Gillani, S.A. 2011b. Phytotoxic effects of selected medicinal plants of Margalla Hills., Islamabad. Pakistan Journal of Medicinal Plants Research 5(18): 4671-4675.

Khattak, A., Ullah, F., Wazir, S.M. & Shinwari, Z.K. 2015. Allelopathic potential of Jatropha Curcas L. leaf aqueous extracts on seedling growth of wheat. Pak. J. Bot. 47(6): 2449-2454.

Krishnaiah, D., Sarbatly, R. & Bono, A. 2007. Phytochemical antioxidants for health and medicine - A move towards nature. Biotechnol. Mol. Biol. Rev. 1(4): 097-104.

Leven, M.D., Vanden-Berghe, D.A., Marten, T., Vilientmick, A. & Lomweas, E.C. 1979. Screening of higher plants for biological activity. Planta Medica 36: 311-312.

Maisuthisakul, P. 2008. Antiradical scavenging activity and polyphenolic compounds extracted from Thai mango seed kernels. Asian J. of Food and Agro-Industry 1(02): 87-96.

Martin, N.J., Ferreiro, S.F., Barbault, F., Nicolas, M., Lecellier, G., Paetz, C., Gaysinski, M., Alonso, E., Thomas, O.P., Botana, L.M. & Raharivelomanana, P. 2015. Indole alkaloids from the Marquesan plant Rauvolfia nukuhivensis and their effects on ion channels. Phytochemistry 109: 84-95.

Mattila, P. & Hellström, J. 2007. Phenolic acids in potatoes, vegetables, and some of their products. J. Food Compos. Anal. 20: 152-160.

Namuli, A., Abdullah, N., Sieo, C.C., Zuhainis, S.W. & Oskoueian, E. 2011. Phytochemical compounds and antibacterial activity of Jatropha curcas Linn extracts. Journal of Medicinal Plants Research 5(16): 3982-3990.

Okujagu, T.F., Etatuvie, S.O., Eze, I., Jimoh, B., Nweke, C. & Mbaoji, C. 2006. Medicinal Plants of Nigeria; South-West Nigeria. In collaboration with Lagos state Traditional Medicine Board and Botany Department, University of Lagos. 1st ed. 1: 20.

Oskoueian, E., Abdullah, N., Saad, W.Z., Omar, A.R., Puteh, M.B. & Ho, Y.W. 2011. Anti-nutritional metabolites and effect of treated Jatropha curcas kernel meal on rumen fermentation in vitro. J. Anim. Vet. Adv. 10: 214-220.

Packer, V.G., Selani, M.M., Gomez, F.G., Ruiz, J.N., Augusto, T.R., Contrerascastillo, C.J. & Alencars, S.M. 2010. Addition of beet and guava agroindustrial residues in cooked and refrigerated chicken meat and its effect on lipid oxidation. Polytechinic University of Valencia, Spain.

Rathee, P., Chaudhary, H., Rathee, S., Rathee, D., Kumar, V. & Kohli, K. 2009. Mechanism of action of flavonoids as anti-inflammatory agents: A review. Inflammation and Allergy Drug Targets 8(3): 229-235.

Rug, M. & Ruppel, A. 2000. Toxic activities of the plant Jatropha curcas against intermediate snail hosts and larvae of Schistosomes. Tropical Medicine & International Health 5: 423-430.

Sher, Z., Hussain, F. & Badshah, L. 2014. Allelopathic potential of Mallotus philippinensis muell. euphorbiaceae. Pak. J. Bot. 46(6): 2057-2061.

Thomas, R., Sah, N. & Sharma, P. 2008. Therapeutic biology of Jatropha curcas: A mini review. Current Pharmaceutical Biotechnology 9(4): 315-324.

Uche, F.I. & Aprioku, J.S. 2008. The phytochemical constituents, analgesic and anti-inflammatory effects of methanol extract of Jatropha curcas leaves in mice and wister albino rats. J. Appl. Sci. Environ. Manage. 12(4): 99-102.

Ullah, F., Bano, A. & Nosheen, A. 2014. Sustainable measures for biodiesel production. Energy Sources, Part A: Recovery, Utilization, and Environmental Effects 36: 2621-2628.

Vasantha, K., Priyavardhini, S., Soris, P. & Mohan, V.R. 2012. Phytochemical analysis and antibacterial activity of Kedrostis foetidissima (Jacq.) Cogn. Bioscience Discovery 3(1): 06-16.

Williamson, G. & Manach, C. 2005. Bioavailability and bioefficacy of polyphenols in humans. II. Review of 93 intervention studies. Am. J. Clin. Nutr. 81(Suppl 1): S243-S255.

Yang, X., Yang, L. & Zheng, H. 2010. Hypolipidemic and antioxidant effects of mulberry (Morus alba L.) fruit in hyperlipidemia rats. Food Chem. Toxicol. 48: 2374-2379.

Yiğit, D., Yiğit, N. & Mavi, A. 2009. Antioxidant and antimicrobial activities of bitter and sweet apricot (Prunus armeniaca L.) kernels. Braz. J. Med. Biol. Res. 42(4): 346-352.

 

 

*Corresponding author; email: drfaizanwazir@gmail.com

 

 

 

 

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