Sains Malaysiana 46(10)(2017): 1727–1734

http://dx.doi.org/10.17576/jsm-2017-4610-08

 

Morphological Characterization and Selection for High Yielding and Powdery Mildew Resistant Pea (Pisum sativum) Lines

(Pencirian Morfologi dan Pemilihan Titisan Kacang Pea (Pisum sativum) untuk Hasil Tinggi dan Tahan Kulapuk Berdebu)

 

 

AJMAL IQBAL1, SHAHEN SHAH2, MOHAMMAD NISAR1* & ABDUL GHAFOOR3

 

1Department of Botany, University of Malakand, Chakdara Dir (Lower), Khyber Pakhtunkhwa, Pakistan

 

2Department of Agronomy, The University of Agriculture, Peshawar, Khyber Pakhtunkhwa, Pakistan

 

3Bio-resources Conservation Institute (BCI), National Agricultural Research Centre (NARS), Islamabad, Pakistan

 

Received: 27 January 2016/Accepted: 26 March 2017

 

ABSTRACT

Twenty lines of Pisum sativum particularly developed for high yield and resistant to powdery mildew were evaluated along with two parents (Falloner and 11760-3ER) and two checks (Climex and a local cultivar) with the objectives to determine morphological characterization, yield potential and resistance to powdery mildew. On the basis of one way cluster, the 24 lines were mainly grouped into four clusters, especially on the vegetative and yield contributing traits. It was observed that the tall and high yielding lines were grouped in cluster-III while the dwarf and high yielding lines were grouped in cluster-IV. Analysis of variance (ANOVA) showed significant difference (p<0.05) in the yield of 24 pea lines. The average grain yield of the 24 pea lines ranged from 22.87 to 102.54 g. The highest grain yield was produced by PL-4 (102.54 g plant-1) followed by PL-5 (82.14 g plant-1). Of the 24 pea lines, two lines (PL-4 and PL-5) were highly resistant to powdery mildew disease. Therefore, the newly developed PL-4 and PL-5 lines were high yielding and highly resistant. Among the 19 morphological traits, six (Eigenvalue >1.0) contributed more than 80% variability among the materials.

 

Keywords: Morphological characterization; pea lines; powdery mildew; statistical analysis; yield potential

 

ABSTRAK

Dua puluh titisan Pisum savitum dibangunkan untuk mendapatkan hasil yang tinggi dan tahan terhadap kulapuk berdebu telah dinilai bersama dua induknya (Falloner dan 11760-3ER) dan dua pengesah (Climex dan kultivar tempatan) dengan objektif untuk mengenal pasti pencirian morfologi, potensi hasilan dan daya tahan terhadap kulapuk berdebu. Berasaskan kelompok sehala, 24 titisan telah dibahagikan kepada empat kelompok terutamanya ciri vegetatif dan penyumbang hasil. Dapat diperhatikan, titisan yang panjang dengan hasil yang tinggi telah dikumpulkan dalam kelompok-III, manakala titisan yang kerdil dengan hasil yang tinggi termasuk dalam kelompok-IV. Analisis varians (ANOVA) menunjukkan perbezaan yang ketara (p<0.05) dalam hasil kesemua 24 titisan kacang pea. Purata hasil bijian daripada 24 titisan kacang pea adalah antara 22.87 kepada 102.54 g. Hasil tertinggi bijian telah keluaran daripada PL-4 (102.54 g tumbuhan-1), diikuti PL-5 (82.14 g tumbuhan-1). Daripada 24 titisan kacang pea, dua titisan (PL-4 dan PL-5) mempunyai daya tahan terhadap penyakit kulapuk berdebu yang tinggi. Maka, titisan PL-4 dan PL-5 yang baru dibangunkan adalah berhasilan tinggi dan mempunyai berdaya tahan yang tinggi. Antara 19 ciri morfologi, enam (nilai Eigen>1.0) telah menyumbangkan lebih daripada 80% perbezaan dalam kesemua bahan yang terhasil.

 

Kata kunci: Analisis statistik; kulapuk berdebu; pencirian morfologi; potensi penghasilan; titisan kacang pea

REFERENCES

 

Able, J.A., Langridge, P. & Milligan, A.S. 2007. Capturing diversity in the cereals: Many options but little promiscuity. Trends Plant Sci. 12(2): 71-79.

Ali, Z., Qureshi, A.S., Ali, W., Gulzar, H., Nisar, M. & Ghafoor, A. 2007. Evaluation of genetic diversity present in pea (Pisum sativum L.) germplasm based on morphological traits, resistance to powdery mildew and molecular characteristics. Pak. J. Bot. 39(7): 2739-2747.

Anonymous. 2010. Project coordinator’s Report of AICRP on MULLARP. Indian Institute of Pulses Research, Kanpur, India.

Balcha, A. 2014. Genetic variation for grain yield of common of common bean (Phaseolus vulgaris L.) in the sole and maize/ bean intercropping system. Asian J. Crop Sci. 6: 158-164.

Bhattacharjee, R. & Dey, U. 2014. An overview of fungal and bacterial biopesticides to control plant pathogens/disease. Afr. J. Microbiol. Res. 8(17): 1749-1762.

Bouhadida, M., Srarfi, F., Saadi, I. & Kharrat, M. 2013. Molecular characterization of pea (Pisum sativum L.) using microsatellite markers. J. App. Chem. 5(1): 57-61.

Cao, A., Xing, L., Wang, X., Yang, X., Wang, W., Sun, Y., Qian, C., Ni, J., Chen, Y., Liu, D., Wang, X. & Chen, P. 2011. Serine/ threonine kinase gene Stpk-V, a key member of powdery mildew resistance gene Pm21, confers powdery mildew resistance in wheat. Proc. Natl. Acad. Sci. USA 108(19): 7727-7732.

Ek, M., Eklund, M., von Post, R., Dayteg, C., Henriksson, T., Weibull, P., Ceplitis, A., Isaac, P. & Tuvesson, S. 2005. Microsatellite markers for powdery mildew resistance in pea (Pisum sativum L.). Hereditas 142: 86-91.

Esiyok, D., Bozokalfa, M.K. & Asciogul, T.K. 2011. Variability, heritability and association analysis in plant traits of Swiss chard (Beta vulgaris subsp. cicla). Genetika 43(2): 239-252.

FAOSTAT. 2012. Food and Agriculture Organization Corporate Statistical Database. http://faostat.fao.org. 2012.

Fondevilla, S. & Rubiales, D. 2012. Powdery mildew control in pea. A review. Agron. Sustain. Dev. 32: 401-409.

Ghafoor, A. & McPhee, K. 2012. Marker assisted selection (MAS) for developing powdery mildew resistant pea cultivars. Euphytica 186: 593-607.

Ghafoor, A. & Arshad, M. 2008. Multivariate analyses for quantitative traits to determine genetic diversity of blackgram (Vigna mungo) germplasm. Pak. J. Bot. 40: 2307-2313.

Ghafoor, A., Ahmad, Z. & Anwar, R. 2005. Genetic diversity in Pisum sativum and a strategy for indigenous biodiversity conservation. Pak. J. Bot. 37(1): 71-77.

Hanaa, H. & Ali, E. 2011. Agronomic performance of seven pea (Pisum sativum) genotypes with five sowing dates in sandy soil. Acta. Agron. Hung. 59(4): 337-347.

Keneni, G., Jarso, M., Wolabu, T. & Dino, G. 2005. Extent and pattern of genetic diversity for morpho-agronomic traits in Ethiopian highland pulse landraces: I. Field pea (Pisum sativum L.). Genet. Res. Crop. Evol. 52: 539-549.

Khan, T.N., Ramzan, A., Jillani, G. & Mehmood, T. 2013. Morphological performance of peas (Pisum sativum) genotypes under rainfed conditions of Potowar region. Afr. J. Agric. Res. 51(1): 51-60.

Kumar, B., Kumar, A., Singh, A.K. & Lavanya, G.R. 2013. Selection strategy for seed yield and maturity in field pea (Pisum sativum L. arvense). Afr. J. Agric. Res. 8(44): 5411- 5415.

Maji, A.T. & Shaibu, A.A. 2012. Application of principal component analysis for rice germplasm characterization and evaluation. J. Plant Breed. Crop Sci. 4(6): 87-93.

McCune, B. & Grace, J.B. 2005. Multivariate Analysis of Ecological Data (PC-ORD ver. 5.10 MjM Software) Gleneden Beach, Oregon United State of America.

Muhammad, S., Bacha, S., Arshad, M., Din, R. & Ghafoor, A. 2009. Genetic diversity for determining yield potential and selection criteria in Pisum sativum L., genetic resources. Pak. J. Bot. 41(6): 2987-2993.

Naghavi, M.R. & Jahansouz, M.R. 2005. Variation in the agronomic and morphological traits of Iranian chickpea accessions J. Integr. Plant Biol. 47: 375-379.

Nemecek, T., von Richthofen, J.S., Dubois, G., Casta, P., Charles, R. & Pahl, H. 2008. Environmental impacts of introducing grain legumes into European crop rotations. Eur. J. Agron. 28: 380-393.

Nisar, M. & Ghafoor, A. 2009. Inheritance studies of Pisum sativum F1, F2 and F3 generation based morphological traits and selection of high yielding powdery mildew resistant lines. Mol. Plant Breed. 7(2): 1-6.

Nisar, M., Ghafoor, A. & Khan, M.R. 2011. Phenotypic variation in the agronomic and morphological traits of Pisum sativum L. germplasm obtained from different parts of the world. Russ. J. Genet. 47(1): 19-25.

Nisar, M., Ghafoor, A., Ahmad, H., Khan, M.R., Qureshi, A.S., Ali, H. & Islam, M. 2008. Evaluation of genetic diversity of pea germplasm through phenotypic trait analysis. Pak. J. Bot. 40(5): 2081-2086.

Nisar, M., Ghafoor, A., Khan, M.R. & Qureshi, A.S. 2006. Screening of Pisum sativum L. germplasm against Erysiphe pisi Syd. Acta. Biol. Cracov. Bot. 48(2): 33-37.

Ouji, A., Rouaissi, M., Abdellaoui, R. & Gazzah, M.E. 2011. The use of reproductive vigor descriptors in studying genetic variability in nine Tunisian faba bean (Vicia faba L.) populations. Afr. J. Biotechnol. 10(6): 896-904.

Shahid, M., Shah, S.F.A., Ghufranulhaq, Ali, H. & Ishtiaq, S. 2010. Resistance in pea germplasm/lines to powdery mildew under natural conditions. Mycopath. 8(2): 77-80.

Sharma, Sood, M., Rana, A. & Singh, Y. 2007. Genetic variability and association studies for green pod yield and component horticultural traits in garden pea under high hill dry temperate conditions. Indian J. Hortic. 64(4): 410-414.

Sillero, J.C., Fondevilla, S., Davidson, J., VazPatto, M.C., Warkentin, T.D., Thomas, J. & Rubiales, D. 2006. Screening techniques and sources of resistance to rusts and mildews in grain legumes. Euphytica 147: 255-272.

Singh, M., Kumar, B. & Singh, J.P. 2007. Genetic variability, heritability and character association in dwarf field pea (Pisum sativum L.). Progress. Agric. 7: 102-104.

Sultana, T. & Ghafoor, A. 2006. Geographical patterns of diversity of cultivated lentil germplasm collected from Pakistan, as assessed buy seed protein assays. Acta. Biol. Cracov. Bot. 48: 77-84.

Tajammal, M.A. & Naz, N. 2009. Morphological descriptor peas (Pisum sativum L.). Federal seed certification and registration department. Ministry of Food and Agriculture. Government of Pakistan, Islamabad. pp. 293-300.

Twari, G. & Lavanya, G.R. 2012. Genetic variability, character association and component analysis in F4 generation of field pea (Pisum sativum var. arvense L.). Karnataka J. Agric. Sci. 25(2): 173-175.

Tzitzikas, E.N., Vincken, J.P., de Groot, J., Gruppen, H. & Visser, R.G. 2006. Genetic variation in pea seed globulin composition. J. Agric. Food Chem. 54(2): 425-433.

Vieira, E.A., de Carvalho, F.I.F., Bertran, I., Kopp, M.M., Zimmer, P.D., Benin, G., da Silva, J.A.G., Hartwig, I., Malone, G. & de Oliveira, A.C. 2007. Association between distances genetic in wheat (Triticum aestivum L.) as estimated by AFLP and morphological markers. Genet. Mol. Biol. 30: 392-399.

 

 

*Corresponding author; email: mnshaalpk@yahoo.com

 

 

 

 

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