Sains Malaysiana 46(6)(2017): 897–902

http://dx.doi.org/10.17576/jsm-2017-4606-08

 

In Vitro Somatic Embryos Multiplication of Eurycoma longifolia Jack using Temporary Immersion System RITA®

(Multiplikasi In Vitro Embrio Somatik daripada Eurycoma longifolia Jack Menggunakan Sistem Rendaman Sementara RITA®)

 

NOOR MADIHAH MOHD, HAFSAH JA'AFAR, DHIYA DALILA ZAWAWI & NADIAWATI ALIAS*

 

School of Agriculture Science and Biotechnology, Faculty of Bioresources and Food Industry, Universiti Sultan Zainal Abidin, Tembila Campus, 22200 Besut, Terengganu Darul Iman, Malaysia

 

Received: 6 March 2016/Accepted: 9 November 2016

 

ABSTRACT

Temporary immersion system, Recipient for Automated Temporary Immersion® (RITA®), is one of the innovative systems that allow the production of large number of somatic embryos or plantlets via in vitro plant propagation. It has been widely used to avoid associated problems such as low multiplication rate of somatic embryos and hyperhydricity during plant propagation. Thus, an attempt was made to investigate optimal parameters such as immersion time and immersion frequency, for the multiplication of direct somatic embryogenesis from cotyledon culture of Eurycoma longifolia Jack using RITA®. Four periods of immersion time (1, 5, 10 and 15 min every 4 h) were evaluated for the efficiency in somatic embryos multiplication. In order to optimize repetitive somatic embryogenesis, three different immersion frequencies (5 min immersion every 2, 4 and 8 h) were applied. The highest number of secondary embryos (69.67 ± 9.73) was found significant when immersing the globular, primary embryos for 5 min every 4 h as compared to other immersion time tested. The secondary somatic embryos obtained in this study could be further used for the development of plantlet regeneration of E. longifolia.

 

Keywords: Eurycoma longifolia Jack; secondary embryogenesis; temporary immersion system RITA®

 

ABSTRAK

Sistem rendaman sementara, ''Recipient for Automated Temporary Immersion®' (RITA®), merupakan salah satu sistem inovatif yang merangsang pengeluaran embrio somatik atau anak pokok dalam kuantiti yang banyak bagi pembiakbakaan in vitro pokok. Sistem ini telah banyak digunakan bagi mengelakkan masalah seperti multiplikasi embrio somatik yang rendah dan hiperhidrisiti bagi pembiakbakaan secara in vitro. Jadi, satu kajian telah dijalankan bagi mengkaji parameter yang optimum seperti tempoh masa dan frekuensi rendaman bagi multiplikasi embrio somatik daripada kultur kotiledon Eurycoma longifolia Jack dengan menggunakan RITA®. Empat tempoh masa rendaman (1, 5, 10 dan 15 min setiap 4 jam) telah dinilai keberkesanannya bagi multiplikasi embrio somatik. Untuk mengoptimumkan penghasilan embrio somatik sekunder, tiga frekuensi rendaman (5 min setiap 2, 4 dan 8 jam) telah digunakan. Jumlah embrio sekunder somatik tertinggi (69.67 ± 9.73) telah diperoleh untuk tempoh rendaman selama 5 min bagi setiap 4 jam jika dibandingkan dengan tempoh masa rendaman yang lain. Embrio somatik sekunder yang diperoleh melalui kajian ini boleh digunakan pada masa hadapan bagi pertumbuhan semula anak pokok E. longifolia.

 

Kata kunci: Embrio somatik sekunder; Eurycoma longifolia Jack; sistem rendaman sementara RITA®

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*Corresponding author; email: nadiawati@unisza.edu.my

 

 

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