Sains Malaysiana 46(8)(2017): 1163–1169

http://dx.doi.org/10.17576/jsm-2017-4608-01

 

Histological Organization of Thyroid and Interrenal Glands of the Pearl Mullet, Alburnus tarichi (Cypriniformes: Cyprinidae) from Lake Van Basin of Turkey

(Pengaturan Histologi Kelenjar Tiroid dan antara Renal Ikan Belanak Mutiara, Alburnus tarichi (Cypriniformes: Cyprinidae) dari lembangan Tasik Van, Turki)

 

 

BURAK KAPTANER*

 

Department of Biology, Faculty of Science, Van Yüzüncü Yil University, 65080 Tusba, Van, Turkey

 

Diserahkan: 6 Jun 2016/Diterima: 6 Januari 2017

 

ABSTRACT

In this study, histological organizations of the thyroid and interrenal glands were first investigated in pearl mullet (Alburnus tarichi Güldenstädt, 1814), living in the Lake Van basin in Turkey. The serial sections taken from the subpharyngeal region and kidney were stained with hematoxylin and eosin, Mallory’s trichrome and periodic acid-Schiff/hematoxylin and then examined. The histological examinations showed that the thyroid gland of pearl mullet was unencapsulated and the thyroid follicles were scattered diffusely in the subpharyngeal region, located at the dorsal and lateral aspects of the ventral aorta. The follicles had colloid-filled lumens and were surrounded by simple follicular epithelial cells varying in shapes (squamose, cuboidal and cylindrical). Thyroid follicles were not found in any areas of the kidney. The interrenal and chromaffin tissue were observed to be associated with postcardinal vein with its branches in the head of the kidney. The interrenal cells having a deeply eosinophilic cytoplasm were determined adjacent to branches in the head kidney. The eosinophobic chromaffin cells were found to show two different distributions. They were observed as being intermingled with the interrenal cells and formed separate groups surrounded by a thin connective tissue in the walls of the major veins. Both interrenal and chromaffin cells displayed a negative periodic acid-Schiff reaction.

 

Keywords: Alburnus tarichi; histology; interrenal gland; thyroid gland

 

ABSTRAK

Dalam kajian ini, pengaturan histologi kelenjar tiroid dan antara renal dikaji kali pertama dalam ikan belanak mutiara (Alburnus tarichi Güldenstädt, 1814) yang hidup di lembangan Tasik Van di Turki. Bahagian keratan yang diambil dari rantau subfarinks dan buah pinggang telah diwarna dengan hematoksilin dan eosin, trikrom Mallory dan asid berkala-Schiff/hematoksilin dan seterusnya dikaji. Pemeriksaan histologi menunjukkan bahawa kelenjar tiroid daripada ikan belanak mutiara telah dikapsul dan folikel tiroid tersebar resap di rantau subfarinks, terletak pada aspek dorsal dan sisian aorta ventral. Folikel mempunyai lümen koloid yang dipenuhi dan dikelilingi oleh pelbagai bentuk (squamose, cuboidal dan silinder). Folikel tiroid tidak ditemui dalam mana-mana bahagian buah pinggang. Tisu dalaman dan kromafin diperhatikan dikaitkan dengan urat pasca kardinal dengan cawangannya di kepala buah pinggang. Sel-sel antara renal yang mempunyai sitoplasma yang sangat eosinofilik, ditentukan berdekatan dengan cabang-cabang dalam kepala buah pinggang. Sel eosinofobik kromafin dilihat menunjukkan dua taburan berbeza. Mereka diperhatikan bercampur dengan sel antara renal dan membentuk kumpulan berasingan yang dikelilingi oleh tisu sambungan nipis di dalam dinding saluran vena utama. Kedua-dua sel antara renal dan kromafin menunjukkan tindak balas asid berkala-Schiff yang negatif.

Kata kunci: Alburnus tarichi; histologi; kelenjar antara renal; kelenjar tiroid

REFERENCES

Abbas, H.H., Authman, M.M., Zaki, M.S. & Mohamed, G.F. 2012. Effect of seasonal temperature changes on thyroid structure and hormones secretion of white grouper (Epinephelus aeneus) in Suez Gulf, Egypt. Life Science Journal 9: 700-705.

Abdel-Aziz El-S. H., El-Sayed Ali, T., Abdu, S.B.S. & Fouad, H.F. 2010. Chromaffin cells and interregnal tissue in the head kidney of the grouper, Epinephilus tauvina (Teleostei, Serranidae): A morphological (optical and ultrastructural) study. Journal of Applied Ichthyology 26: 522-527.

Aida, K., Kaneko, T., Oguri, M. & Sato, H. 1995. Endocrine system. In An Atlas of Fish Histology, 2nd. ed., edited by Takashima, F. & Hibiya, T. Tokyo-Stuttgart New York: Kodansha-Gustav Fischer Verlag. pp. 154-185.

Chakrabarti, P. & Ghosh, S.K. 2014. Cyclical changes in interrenal and chromaffin cells in relation to testicular activity of olive barb, Puntius sarana (Hamilton). Archives of Polish Fisheries 22: 151-158.

Chakrabarti, P. 2014. Histological features of interrenal and chromaffin cells in relation to seasonal testicular activities in Notopterus notopterus (Pallas, 1769). Iranian Journal of Ichthyology 1: 206-213.

Civinini, A., Padula, D. & Gallo, V.P. 2001. Ultrastructure and histochemical study on the interrenal cells of the male stickleback (Gasterosteus aculeatus, Teleostea), in relation  to the reproductive annual cycle. Journal of Anatomy 199: 303-316.

Danulat, E. & Selcuk, B. 1992. Life history and environmental conditions of the anadromous Chalcalburnus tarichi (Cyprinidae) in the highly alkaline lake Van, Eastern Anatolia, Turkey. Archiv fur Hydrobiologie 126: 105-125.

Durkina, V.B. 2006. The state of the interrenal gland in plaices Pleuronectes pinnifasciatus and P. obscurus from two stations in Amurskii Bay (Sea of Japan). Journal of Ichthyology 46: 195-199.

Eales, J.G. & Brown, S.B. 1993. Measurement and regulation of thyroidal status in teleost fish. Reviews in Fish Biology and Fisheries 4: 299-347.

Gallo, V.P. & Civinini, A. 2003. Survey of the adrenal homolog in teleosts. International Review of Cytology 230: 89-187.

Gazola, R., Borella, M.I., Val-Sella, M.V., de Moraes F.F. & Bernardino, G. 1995. Histophysiological aspects of the interrenal gland of the pacu female, Piaractus mesopotamicus (Teleostei, Cypriniformes). Boletim Técnico do CEPTA, Pirassununga 8: 1-12.

Genten, F., Terwinghe, E. & Danguy, A. 2008. Endocrine Glands. In Atlas of Fish Histology (Chapter 13). Enfield, NH, USA: Science Publishers. p. 223.

Geven, E.J.W., Nguyen, N.K., van den Boogaart, M., Spanings, F.A.T., Flik, G. & Klaren, P.H.M. 2007. Comparative thyroidology: Thyroid gland location and iodothyronine dynamics in Mozambique tilapia (Oreochromis mossambicus Peters) and common carp (Cyprinus carpio L.). Journal of Experimental Biology 210: 4005-4015.

Janz, D.M. 2000. Endocrine system. In The Laboratory Fish, edited by Ostrander, G.K. San Diego: Academic Press. pp. 189-217.

Kaptaner, B. & Kankaya, E. 2013. Analysis of germ cell proliferation, apoptosis, and androgenesis in the Lake Van fish (Chalcalburnus tarichi) during testicular development. Fish Physiology and Biochemistry 39: 1165-1679.

Leatherland, J.F. 1994. Reflections on the thyroidology of fishes: From molecules to humankind. Guelph Ichthyology Reviews 2: 1-64.

Misra, S.K. 1991. Histo-enzymological analysis on the interrenal tissue of the teleost, Anabas scandens (Bloch). Indian Biol. 23: 28-30.

Morandini, L., Honji, R.M., Ramallo, M.R., Moreira, R.G. & Pandolfi, M. 2014. The interrenal gland in males of the cichlid fish Cichlasoma dimerus: Relationship with stress and the establishment of social hierarchies. General and Comparative Endocrinology 195: 88-98.

Morovvati, H., Mahabady, M.K. & Shahbazi, S. 2012. Histomorphological and anatomical study of kidney in berzem (Barbus pectoralis). International Journal of Fisheries and Aquaculture 4: 221-227.

Nishioka, R.S., Grau, E.G., Lai, K.V. & Bern, H.A. 1987. Effect of thyroid-stimulating hormone on the physiology and morphology of the thyroid gland in coho salmon, Oncorhynchus kisutch. Fish Physiology and Biochemistry 3: 63-71.

Ortiz Delgado, J.B., Ruane, N.M., Pousão-Ferreira, P., Dinis, M.T. & Sarasquete, C. 2006. Thyroid gland development in Senegalese sole (Solea senegalensis Kaup 1858) during early life stages: A histochemical and immunohistochemical approach. Aquaculture 260: 346-356.

Raine, J.C. 2011. Thyroid hormones and reproduction. In Hormones and Reproduction of Vertebrates. Volume 1- Fishes, Chap. 25, edited by Norris, D.O. & Lopez, K.H. Elsevier: Academic Press.

Raine, J.C., Takemura, A. & Leatherland, J.F. 2001. Assessment of thyroid function in adult medaka (Oryzias latipes) and juvenile rainbow trout (Oncorhynchus mykiss) using immunostaining methods. Journal of Experimental Zoology 290: 366-378.

Raine, J.C. & Leatherland, J.F. 2000. Morphological and functional development of the thyroid tissue in rainbow trout (Oncorhynchus mykiss) embryos. Cell and Tissue Research 301: 235-244.

Rocha, R.M., Leme-Dos Santos, H.S., Vicentini, C.A. & Da Cruz, C. 2001. Structural and ultrastructural characteristics of interrenal gland and chromaffin cell of Matrinxã, Brycon cephalus Gunther 1869 (Teleostei-Characidae). Anatomia Histolologia Embryologia 30: 351-355.

Sampour, M. 2008. The study of adrenal chromaffin of fish, Carassius auratus (Teleostei). Pakistan Journal of Biological Sciences 11: 1032-1036.

Senarat, S., Kettratad, J., Lampang, P.N., Gettongsong, T., Karnjanapak, C., Palasai, A., Kangwanrangsan, N. & Jiraungkoorskul, W. 2016. Structural organization of the thyroid gland and interrenal tissue with reference to endocrine parenchyma in short mackerel, Rastrelliger brachysoma (Bleeker, 1851). Agriculture and Natural Resources 50: 60-63.

Wendl, T., Lun, K., Mione, M., Favor, J., Brand, M., Wilson, S.W. & Rohr, K.B. 2002. Pax2.1 is required for the development of thyroid follicles in zebrafish. Development 129: 3751-3760.

Yoakim, E.G. & Grizzle, J.M. 1980. Histological, histochemical and ultrastructural studies on the interrenal and chromaffin cells of the fathead minnow, Pimephales promelas Rafinesque. J. Fish Biol. 17: 477-494.

 

 

*Pengarang untuk surat-menyurat; email: bkaptaner@yahoo.com