Sains Malaysiana 47(5)(2018): 909–921

http://dx.doi.org/10.17576/jsm-2018-4705-06

 

Identification of Phytophthora spp. from Perennial Crops in Malaysia, Its Pathogenicity and Cross-pathogenicity

(Pengecaman Pencilan Phytophthora spp. daripada Tanaman Saka Berkayu di Malaysia, Kepatogenan dan Silang Kepatogenannya)

 

M. LATIFAH, S. KAMARUZAMAN, M.A. ZAINAL ABIDIN & S.A. NUSAIBAH*

 

Department of Plant Protection, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia

 

Received: 22 November 2016/Accepted: 29 December 2017

 

 

ABSTRACT

Phytophthora is one of the most important plant pathogens of perennial crops in the tropics. This study characterised the pathogen(s) responsible for cocoa black pod rot, rubber pod rot and durian stem canker. Eighteen Phytophthora isolates were collected from several states in Malaysia, namely Pahang, Johor and Selangor. A total of 12 Phytophthora palmivora and six Phytophthora nicotianae isolates were isolated and identified based on the morphological and molecular characteristics. Internal transcribed spacer (ITS) sequences enabled Phytophthora identification to species level. Inoculation of 18 isolates on detached leaves and unripen cocoa pods successfully demonstrated the progressive development of lesions on its original host. Lesions were also observed in all wounded detached leaves of young durian (clone D24) and rubber (clone RRIM600) and unripen cocoa pods (clone BAL244) regardless of host and isolates. Phytophthora isolates from durian (DSCI) and rubber (R4A) used to inoculate cocoa pods exhibited lesion sizes of 11.6±0.75 and 9.6±0.64 mm, respectively. However, Phytophthora isolates from cocoa (CPR25) developed a longer length of lesion of 18.6±0.47 mm. On durian leaves, Phytophthora isolate from rubber (R4A) and cocoa (CPR25) recorded 11.73±1.04 and 5.22±0.57 mm length of lesion while Phytophthora isolates from durian (DSCB4) produced 13.13±1.29 mm lesion on its native host. Isolates from cocoa (CPR22) and durian (DSCE3) infected rubber leaves with 3.74±0.48 and 2.55±0.31 mm length of lesion, in comparison to rubber isolates (R1B) with 5.43±0.23 mm length of lesion. Differences in the length of lesion demonstrated higher level of virulence on the native host.

 

Keywords: Cross-pathogenicity; internal transcribed spacers (ITS); pathogenicity; phylogenetic tree; Phytophthora

 

ABSTRAK

Phytophthora merupakan patogen tumbuhan yang penting kepada tanaman saka berkayu di kawasan beriklim tropika. Pencirian dan pengenalpastian terhadap patogen Phytophthora spp. yang merupakan penyebab penyakit buah hitam pada tanaman koko, reput pada buah getah dan kanker batang pada pokok durian telah dijalankan di dalam kajian ini. Sejumlah 18 pencilan Phytophthora spp. telah diperoleh daripada sampel tisu berpenyakit dari beberapa buah negeri di Malaysia iaitu Johor, Pahang dan Selangor. Dua belas pencilan telah dikenal pasti sebagai Phytophthora palmivora dan enam lagi pencilan sebagaiPhytophthora nicotianae berdasarkan sifat morfologi, kultur dan pencirian secara molekul. Pencirian molekul pada kawasan jujukan internal transcribed spacer (ITS) membolehkan pengecamanPhytophthora sehingga ke peringkat spesies. Inokulasi 18 pencilan Phytophthora spp. pada helaian daun dan buah koko yang belum matang menunjukkan kesan lesi merebak pada sampel berkenaan. Kesan lesi hadir pada kawasan luka helaian daun muda durian (klon D24), getah (klon RRIM600) dan buah koko yang belum matang (klon BAL244) tanpa mengambil kira faktor hos dan pencilan. PencilanPhytophthora daripada durian (DSCI) dan getah (R4A) yang digunakan untuk menginokulat buah koko yang belum matang telah mempamirkan saiz lesi masing-masing 11.6±0.75 dan 9.6±0.64 mm. Walau bagaimanapun, pencilanPhytophthora daripada koko (CPR25) mempamerkan kesan lesi yang lebih panjang (18.6±0.47 mm). Pada daun durian, pencilanPhytophthora daripada getah (R4A) dan koko (CPR25) telah mempamerkan kesan lesi bersaiz masing-masing 11.73±1.04 dan 5.22±0.57 mm. Sementara itu, pencilanPhytophthora daripada pencilan durian (DSCB4) telah menghasilkan saiz lesi 13.13±1.29 pada hos asalnya iaitu daun durian. Pencilan daripada koko (CPR22) dan durian (DSCE3) yang diinokulat pada daun getah telah mempamerkan saiz lesi masing-masing 3.74±0.48 dan 2.55±0.31 mm. Namun, lesi yang lebih panjang (5.43±0.23 mm) diperhatikan pada daun getah yang diinokulat dengan pencilan (R1B) daripada getah. Perbezaan panjang lesi yang diperhatikan telah menunjukkan tahap kevirulenan yang tinggi pada perumah asalnya.

 

Kata kunci: Internal transcribed spacer (ITS); kepatogenan; Phytophthora; pohonfilogeni; silang-kepatogenan

REFERENCES

Belgrave, W.N.C. & Norris, F.D.L.M. 1917. Notes on bark cankers and their treatment. F.M.S. Agric. Bull. 6: 2-10.

Boccas, B.R. 1973. Observations preliminaires sur l'héredité du pouvoir pathogène chez le P. palmivora (Butl.) Butl. Cah. ORSTOM sér. Bio.20: 51-56.

Bowman, K.D., Albrecht, U., Graham, J.H. & Bright, D.B. 2007. Detection of Phytophthora nicotianaeand P. palmivora in citrus roots using PCR-RFLP in comparison with other methods. Eur. J. Plant Pathol. 119: 143-158.

Brasier, C.M. & Griffin, M.J. 1979. Taxonomy of 'Pytophthora palmivora' on cocoa. Trans. Br. Mycol. Soc. 72: 111-143.

Chee, K.H. 1973. Production, germination and survival of chlamydospores of Phytophthora palmivorafrom Hevea brasiliensis. Trans. Br. Mycol. Soc. 6: 21-26.

Chillali, M., Idder-Ighili, H., Guillaumin, J.J., Mohammed, C., Escarmant, L. & Botton, B. 1998. Variation in the ITS and IGS regions of ribosomal DNA among the biological species of European Armillaria. Mycol. Res. 102(5): 533-540.

Cooke, D.E.L., Drenth, A., Duncan, J.M., Wagels, G. & Brasier, C.M. 2000. A molecular phylogeny of Phytophthora and related oomycetes. Fungal Genet. Bio.30: 17-32.

Crawford, A.R., Bassam, B.J., Drenth, A., MacLean, D.J. & Irwin, J.A.G. 1996. Evolutionary relationships among Phytophthora species deduced from rDNA sequence analysis. Mycol. Res. 100: 437-443.

Drenth, A. & Sendall, B. 2004. Economic impact of Phytophthora diseases in Southeast Asia. In Diversity and Management of Phytophthora in Southeast Asia, edited by Drenth, A. & Guest, D.I. ACIAR Monograph. 114: 10-28, 97-99.

Edathil, T.T. & George, M.K. 1976: Phytophthora nicotianaevar. parasitica (Dastur) Waterhouse on Hevea brasiliensisin South India. Rubb. Board Bull. 13: 3-4.

Erwin, D.C. & Ribeiro, O.K. 1996. Phytophthora Diseases Worldwide. St. Paul, Minnesota: The American Phytopathological Society.

Förster, H., Cummings, M.P. & Coffey, M.D. 2000. Phylogenetic relationship of Phytophthora species based on ribosomal ITS I DNA sequence analysis with emphasis on Waterhouse groups V and VI. Mycol. Res. 104: 1055-1061.

Hall, G.1993. An integrated approach to the analysis of variation in Phytophthora nicotianae and a redescription of the species. Mycol. Res. 97: 559-574.

Henson, J.M. & French, R. 1993. The polymerase chain reaction and plant disease diagnosis. Annu. Rev. Phytopathol. 31: 81-109.

Hee, W.Y., Torreña, P.S., Blackman, L.M. & Hardham, A.R. 2013. Phytophthora cinnamomiin Australia. In Phytophthora: A Global Perspective, edited by Lamour, K. UK: CAB International. pp. 124.

Hibbett, D.S. 1992. Ribosomal RNA and fungal systematics. Trans. Mycol. Soc. Japan 33: 533-556.

Ko, W.S. & Ho, W.C. 1997. A simple method for obtaining single-spores isolates of fungi. Bot. Bull. Acad. Sinica. 38: 41-44.

Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. 1990. PCR Protocols: A Guide to Methods and Applications. New York: Academic Press, Inc.

Jiang, R.H., Tripathy, S., Govers, F. & Tyler, B.M. 2008. RXLR effector reservoir in two Phytophthora species is dominated by a single rapidly evolving superfamily with more than 700 members. Proceedings of the National Academy of Sciences of the United States of America 105: 4874-4879.

Kennedy, D.M. & Duncan, J.M. 1995. A papillate Phytophthora species with specificity to Rubus. Mycol. Res. 99: 57-68.

Lee, B.S. & Varghese, G. 1974. Studies on the genus Phytophthora in Malaysia. II. Reproduction and sexuality. Malay. Agric. Res. 3: 137-149.

Lee, S.B. & Taylor, J.W. 1992. Phylogeny of five fungus-like protoctistan Phytophthora species, inferred from the internal transcribed spacers of ribosomal DNA. Mol. Biol. Evol. 9: 636-653.

Liu, P.S.W. 1977.  Diseases caused by Phytophthora and Pythium in Sabah, Malaysia. Tech. Bull. 3: 48.

MacDonald, J.D., Stites, J. & Kabashima, J. 1990. Comparison of serological and culture plate methods for detection species of Phytophthora, Pythium, and Rhizoctonia in ornamental plants. Plant Dis. 74: 655-659.

McMahon, P. & Purwantara, A. 2004. Phytophthora on cocoa. In Diversity and Management of Phytophthora in Southeast Asia, edited by Drenth, A. & Guest, D.I. ACIAR Monograph 114: 104-115.

Navaratnam, S.J. 1966. Patch canker of the durian tree. Malay. Agric. J. 45: 291-294.

Newhook, F.J. 1978. Phytophthora cinnamomi in native forests of Australia and New Zealand: Indigenous or introduced? In Microbial Ecology, edited by Loutit, M.W. & Heidelberg, J.A.R. Germany: Springer-Verlag.

Oliver, R.P. 1993. Nucleic acid-based methods for detection and identification. In Principles of Diagnostic Techniques in Plant Pathology, edited by Fox, R.T.V. Wallingford, UK: CAB International. pp. 153-170.

Raffaele, S., Farrer, R.A. & Cano, L.M. 2010. Genome evolution following host jumps in the Irish potato famine pathogen lineage. Science 330: 1540-1543.

Seidl, M.F., van den Ackerveken, G., Govers, F. & Snel, B. 2011. A domain-centric analysis of oomycete plant pathogen genomes reveals unique protein organization. Plant Physiol. 155: 628-644.

Shearer, B.L., Crane, C.E. & Cochrane, A. 2004. Quantification of the susceptibility of the native flora of the South-West Botanical Province, Western Australia, to Phytophthora cinnamomi. Aust. J. Bot. 52: 435-443.

Slamet, A.R. 1991. Pathogenicity test of three isolates of Phytophthora palmivoraon black pepper, coconut, cacao and vanilla. Bulletin Komunikasi Penetilian Rempah dan Obat. p. 6.

Stamps, D.J., Waterhouse, G.M., Newhook, F.J. & Hall, G.S. 1990. Revised tabular key to the species of Phytophthora. Mycol. Papers 162: 1-28.

Suzuki, T.J., Kueprakone, U. & Kamphangridthrong, T. 1979. Phytophthora spp. isolated from some economic plants in Thailand. Tech. Bull. Trop. Agric. Res. Center Jpn. 12: 32-41.

Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. 2013. MEGA 6: Molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30(12): 2725-2729.

Taylor, J.W., Jacobson, D.J., Kroken, S., Kasuga, T., Geiser, D.M., Hibbett, D. & Fisher, M.C. 2000. Phylogenetic species recognition and species concepts in fungi. Fungal Genet. Biol. 31: 21-32.

Thankamma, L. 1974. Phytophthora nicotianaevar. nicotianae on Anacardium occidentalein south India. Plant Dis. Rep. 58: 767-768.

Thankamma, L. 1983. Phytophthora species on eight indigenous host species in south India and their pathogenicity on rubber. Indian Phytopathol. 36: 17-23.

Tsao, P.H. & Ocana, G. 1969. Selective isolation of species of Phytophthora from natural soils on an improved antibiotic medium. Nature 223: 636-638.

Tsao, P.H. & Tummakate, R. 1977. The identity of a Phytophthora species from black pepper in Thailand. Mycologia 69: 631- 637.

Waterhouse, G.M. 1974. Phytophthora palmivora and some related species. In Phytophthora Disease of Cocoa, edited by Gregory, P.H. London: Longman. pp. 51-70.

Waterhouse, G.M. 1963. Key to the species of Phytophthora de Bary. Mycol. Papers. 92: 1-22.

Waterhouse, G.M., Newhook, F.J. & Stamps, D.J. 1983. Present criteria for classification of Phytophthora. In Phytophthora: Its Biology, Taxonomy, Ecology, and Pathology, edited by Erwin, D.C., Bartnicki-Garcia, S. & Tsao, P.H. St. Paul, Minnesota: The American Phytopathological Society. pp. 139-147.

Weste, G. 1983. Population dynamics and survival of Phytophthora. In Phytophthora: Its Biology, Taxonomy, Ecology, and Pathology, edited by Erwin, D.C., Bartnicki- Garcia, S. & Tsao, P.H. St. Paul, Minnesota: The American Phytopathological Society. pp. 139-147.

White, T.J., Bruns, T., Lee, S. & Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: A Guide to Methods and Applications, edited by Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. San Diego: Academic Press. pp. 315-322.

 

 

*Corresponding author; email: nusaibah@upm.edu.my

 

 

 

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