Sains Malaysiana 50(4)(2021): 945-952

http://doi.org/10.17576/jsm-2021-5004-06

Grape Seed Proanthocyanidin Improves Lipopolysaccharide-Induced Myocardial Toxicity in Mice

(Proantosianidin Biji Anggur Menambahbaik Ketoksikan Miokardium pada Tikus yang Diaruh Lipopolisakarida)

LIU XIANCHU^1,2, LI SHA¹, CHEN RUYAN¹, LI YUE¹, PENG HUAN³ & LIU MING¹*

¹Department of Human Movement Science, Institute of Physical Culture, Hunan University of Arts and Science, 415000 Changde, Hunan, People’s Republic of China

²Key Laboratory of Physical Fitness and Exercise Rehabilitation of Hunan Province, Hunan Normal University, 410081 Changsha, People’s Republic of China

³Department of Hematology, Xiangya Changde Hospital, 415000 Changde, Hunan, People’s Republic of China

Received: 16 May 2019/Accepted: 15 September 2020

ABSTRACT

Lipopolysaccharide (LPS) is an important pathogenic factor for sepsis which results in cardiovascular diseases and even mortality. Proanthocyanidin, one of the main components in grape seed, has a wide range of biological activities in various diseases. The mouse model was established by intraperitoneal injection with LPS. Grape seed proanthocyanidin (GSP) was administered continuously for 8 days. Our results showed that pre-treatment of GSP dramatically ameliorated the level of creatine kinase (CK) and lactate dehydrogenase (LDH) to defend against LPS-induced myocardial toxicity (p<0.01). Moreover, pre-treatment of GSP significantly mitigated the expression of matrix metallopeptidase 2 (MMP-2) and matrix metallopeptidase 9 (MMP-9) to inhibit LPS-induced myocardial fibrosis (p<0.01). In addition, pre-treatment of GSP significantly increased superoxide dismutase (SOD) and catalase (CAT) activities to improve the level of malondialdehyde (MDA) and hydrogen peroxide (H₂O₂) (p<0.01). Lastly, pre-treatment of GSP relieved tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6) activities to prevent inflammatory responses (p<0.01).

Keywords: Anti-inflammatory; anti-oxidant; grape seed proanthocyanidin; heart; lipopolysaccharide

ABSTRAK

Lipopolisakarida (LPS) adalah faktor patogen penting untuk sepsis yang mengakibatkan penyakit kardiovaskular dan juga kematian. Proantosianidin iaitu salah satu komponen utama dalam biji anggur mempunyai julat aktiviti biologi yang luas dalam pelbagai penyakit. Model tikus dibangunkan melalui suntikan intraperitoneum menggunakan LPS. Proantosianidin biji anggur (GSP) diberikan secara berterusan selama 8 hari. Hasil kajian kami menunjukkan bahawa pra-rawatan GSP meningkatkan tahap kreatina kinase (CK) dan laktat dehidrogenase (LDH) dengan secara dramatik untuk mempertahankan diri terhadap ketoksikan miokardium yang diaruh oleh LPS (p <0.01). Tambahan pula, pra-rawatan GSP secara signifikan mengurangkan pengekspresan matriks metallopeptida 2 (MMP-2) dan matriks metallopeptida 9 (MMP-9) dengan signifikan untuk menghalang fibrosis miokardium yang diaruh LPS (p <0.01). Di samping itu, pra-rawatan GSP meningkatkan aktiviti superoksida dismutase (SOD) dan katalase (CAT) dengan signifikan untuk meningkatkan tahap malondialdehida (MDA) dan hidrogen peroksida (H₂O₂) (p <0.01). Akhir sekali, pra-rawatan GSP menekan aktiviti faktor nekrosis tumor alfa (TNF-α) dan interleukin 6 (IL-6) untuk mencegah tindak balas keradangan (p <0.01).

Kata kunci: Anti-oksidan; anti-radang; jantung; lipopolisakarida; proantosianidin biji anggur

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*Corresponding author; email: liuminghappy36@163.com