Sains Malaysiana 42(11)(2013): 1549–1555


Synergistic and Antagonistic Effects of Zinc Bioaccumulation with Lead and

Antioxidant Activities in Centella asiatica

(Kesan Sinergistik dan Antagonistik oleh Bioakumulasi Zink dengan Plumbum dan

Aktiviti Antioksidan di Centella asiatica)



G.H. ONG1, C.K. YAP1*, M. MAZIAH2 & S.G. TAN3


1Department of Biology, Faculty of Science, Universiti Putra Malaysia

43400 UPM, Serdang, Selangor, Malaysia


2Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences

Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia


3Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences

Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

Diserahkan: 13 Jun 2012 /Diterima: 9 April 2013



This study was carried out by using Centella asiatica grown using a hydroponic system under laboratory conditions to study synergistic and antagonistic effects of Zn bioaccumulation with added Pb and the changes in antioxidant activities in leaves and roots of C. asiatica. The antioxidant activities included superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and guaiacol peroxidase (GPX). The treatments Zn (2 ppm) + Pb (0.4 ppm) and Zn (4 ppm) + Pb (0.6 ppm) increased the accumulation of Zn in leaves by 14.06  and 16.84%, respectively, but decreased by 7.36% uptake in roots (Zn 4 ppm + Pb 0.6 ppm). This showed that Pb and Zn acted synergistically to Zn accumulation in leaves but antagonistically in roots. CAT and SOD activities in leaves were increased when Zn was added together with Pb. In roots, CAT, APX and SOD activities were increased but GPX was decreased. Owing to their sensitivities to Zn with Pb, SOD and CAT could be used as biomarkers to monitor the toxicity of Pb and Zn exposure in the leaves and roots of C. asiatica.


Keywords: Antagonistic; antioxidant activities; Centella asiatica; synergistic



Kajian ini telah dijalankan dengan Centella asiatica secara hidroponik dalam keadaan makmal untuk mengkaji kesan sinergistik dan antagonistik bioakumulasi Zn ditambah dengan Pb dan perubahan dalam aktiviti antioksidan dalam daun dan akar C. asiatica. Aktiviti antioksidan termasuk superokside dimustase (SOD), katalase (CAT), peroksidase askorbat (APX) dan peroksidase guaiacol (GPX). Rawatan Zn (2 ppm) + Pb (0.4 ppm) dan Zn (4 ppm) + Pb (0.6 ppm) menunjukkan pengumpulan Zn dalam daun sebanyak 14.06  dan 16.84% masing-masing tetapi menurun sebanyak 7.36% dalam pengambilan akar (Zn 4 ppm + Pb 0.6 ppm). Ini menunjukkan bahawa Pb dan Zn bertindak secara sinergistik untuk pengumpulan Zn dalam daun tetapi antogonistik dalam akar. Aktiviti CAT dan SOD dalam daun meningkat apabila Zn ditambah bersama dengan Pb. Dalam akar, CAT, APX dan SOD telah meningkat tetapi GPX telah menurun. Disebabkan sentiviti kepada Zn dengan Pb, SOD dan CAT boleh digunakan sebagai penanda biologi untuk memantau ketoksikan pendedah Pb dan Zn dalam daun dan akar C. asiatica.


Kata kunci: Aktiviti antioksidan; antagonistik; Centella asiatica; sinergistik



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