Sains Malaysiana 43(3)(2014): 407–413

 

Development and Validation of Gas Chromatography-Mass Spectrometric Method for the Determination of Epoxidized Soybean Oil in Foods Stored in Glass Jars with Metal Lids

(Pembangunan dan Validasi Kaedah Kromatografi Gas-Spektrometri Jisim bagi Penentuan

Minyak Kacang Soya Terepoksi dalam Makanan yang Disimpan

di dalam Balang Kaca Berpenutup Logam)

HONG KEAT QUEK1, MOHD MARSIN SANAGI1 2*, WAN AINI WAN IBRAHIM1,

SYAIRAH SALLEH1& MAZIDATULAKMAM MISKAM1 2

 

1Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia

81310 UTM Johor Bahru, Johor, Malaysia

 

2Ibnu Sina Institute for Fundamental Science Studies, Universiti Teknologi Malaysia

81310 UTM Johor Bahru, Johor, Malaysia

 

Diserahkan: 28 Jun 2012/Diterima: 6 Julai 2013

 

ABSTRACT

A method has been developed for the determination of epoxidized soybean oil (ESBO) in oily foods stored in glass jars with metal lids using gas chromatography-mass spectrometry (GC-MS). ESBO and its internal standard (cis,cis-11,12;14,15-diepoxyeicosanoate) were isolated from the matrix by transesterification process. The developed method showed good linear dynamic range between 0.7-20 μg mL-1 with coefficients of determination (R2) > 0.9968 and acceptable limit of detection and limit of quantitation of 7 and 23 mg kg-1, respectively, based on linearity calculations. Analyte recoveries were 90.84±27.24% for low concentration, 78.05±11.59% for medium concentration and 99.23±10.20% for high concentration. This first fully validated GC-MS method was successfully applied for the determination of ESBO in foods stored in glass jar with metal lid. Among the 31 food samples studied, 6 samples were found to exceed the specific migration limit of 60 mg kg-1 (based on EU Directive 2002/72/EC). The developed method is thus potentially useful for routine analysis for the determination of ESBO.

 

Keywords: Epoxidized soybean oil; foods stored in glass jars with metal lids; gas chromatography-mass spectrometry

ABSTRAK

Satu kaedah telah dibangunkan untuk menguji minyak kacang soya terepoksi (ESBO) dalam makanan berminyak di dalam balang kaca berpenutup logam menggunakan kromatografi gas-spektrometri jisim (GC-MS). ESBO dan piawai dalaman (cis,cis-11,12;14,15-diepoxyeicosanoate) telah dipisahkan daripada matrik menggunakan proses transesterifikasi. Kaedah yang dibangunkan ini menunjukkan dinamik linear yang baik antara 0.7-20 μg mL-1 dengan pekali penentuan (R2) > 0.9968 dan had pengesanan serta had kuantifikasi yang boleh diterima iaitu 7 dan 23 mg kg-1, masing-masing berdasarkan kepada pengiraan lineariti. Peratus pengembalian analit ialah 90.84±27.24%, bagi kepekatan rendah, 78.05±11.59% bagi kepekatan sederhana dan 99.23±10.20% bagi kepekatan tinggi. Kaedah GC-MS yang telah divalidasi sepenuhnya buat pertama kali telah berjaya diaplikasikan untuk penentuan ESBO dalam makanan yang disimpan di dalam balang kaca berpenutup logam. Daripada 31 sampel yang telah dikaji, 6 sampel didapati melebihi had 60 mg kg-1 mengikut undang-undang Eropah (EU Directive 2002/72/EC). Oleh itu, kaedah ini berpotensi untuk digunakan sebagai analisis rutin untuk penentuan ESBO.

 

Kata kunci: Kromatografi gas-spektrometri jisim; makanan disimpan di dalam balang kaca berpenutup logam; minyak kacang soya epoksi

 

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*Pengarang untuk surat-menyurat; email: marsin@kimia.fs.utm.my

 

 

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