Sains Malaysiana 45(8)(2016): 1221–1225

Formation of ZnO Nanorods via Low Temperature Hydrothermal Method for Enzymatic Glucose Sensor

(Pembentukan Batang Nano ZnO melalui Kaedah Hidroterma Suhu Rendah untuk Sensor Enzim Glukosa)

 

NUR SYAFINAZ RIDHUAN1, KHAIRUNISAK ABDUL RAZAK1* & ZAINOVIA LOCKMAN1,2

 

1School of Materials and Mineral Resources Engineering, Universiti Sains Malaysia,

14300 Nibong Tebal, Pulau Pinang, Malaysia

 

2NanoBiotechnology Research & Innovation (NanoBRI), INFORMM, Universiti Sains Malaysia

11800 USM, Penang, Malaysia

 

Diserahkan: 20 April 2015/Diterima: 16 November 2015

 

ABSTRACT

In this study, zinc oxide (ZnO) nanorod arrays were synthesized using a simple hydrothermal reaction on a ZnO seeds/ITO substrate and applied for the fabrication of enzymatic glucose sensor. ZnO nanorod matrix provided a favourable environment for the immobilization of glucose oxidase (GOx) and introduced a shuttling way for electronic communication between GOx and electrode. The performance of different aspect ratio of ZnO nanorods that was produced by varying hydrothermal reaction time was studied. The aspect ratio of ZnO influenced the GOx enzyme immobilization. The morphology and structure of prepared ZnO nanorods were characterized by employing scanning electron microscopy (SEM), and X-ray powder diffraction (XRD). Electrochemical measurements of the sensor showed a reproducible sensitivity of 2.06 μA/cm2mM for ZnO matrix grown for 4 h with the aspect ratio of 8.0.

 

Keywords: Cyclic-voltammetry; glucose oxidase (GOx); hydrothermal; ZnO nanorods

 

ABSTRAK

Dalam kajian ini, zink oksida (ZnO) batang nano telah disintesis menggunakan reaksi mudah hidroterma pada benih ZnO/substrat ITO substrat dan digunakan untuk fabrikasi sensor glukosa enzim. ZnO matriks batang nano menyediakan persekitaran yang menggalakkan bagi imobilisasi glukosa oksidase (GOx) seterusya bagi menyediakan laluan komunikasi elektronik antara GOx dan elektrod. Prestasi bagi nisbah aspek batang nano ZnO yang berbeza yang dihasilkan dengan mengubah masa tindak balas hidroterma telah dikaji. Nisbah aspek ZnO mempengaruhi immobilisasi GOx enzim. Morfologi dan struktur ZnO batang nano telah dicirikan dengan menggunakan mikroskop imbasan elektron (SEM) dan pembelauan sinar-X (XRD). Ukuran sensor elektrokimia menghasilkan kesensitifan sebanyak 2.06 µA/cm2mM untuk matrik ZnO yang dihasilkan dengan tindak balas hidroterma selama 4 jam dengan nisbah aspek 8.0.

 

Kata kunci: Batang nano ZnO; glukosa oksidase (GOx); hidroterma; kitar-voltan

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*Pengarang untuk surat-menyurat; email: khairunisak@usm.my

 

 

 

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