Sains Malaysiana 46(9)(2017): 1565–1571

http://dx.doi.org/10.17576/jsm-2017-4609-27

 

Antioxidant and Antimicrobial Activity of the Extracts from Different Parts of Etlingera sayapensis (Zingiberaceae)

(Aktiviti Antioksida dan Antimikrob bagi Ekstrak daripada Bahagian Berbeza Etlingera sayapensis (Zingiberaceae))

 

BEHNAM MAHDAVI1, W.A. YAACOB2* & LAILY B. DIN2

 

1Department of Chemistry, Faculty of Science, Hakim Sabzevari University, Sabzevar, Iran

 

2School of Chemical Sciences and Food Technology, Faculty of Science and Technology

Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

 

Diserahkan: 9 April 2015/Diterima: 5 Mac 2017

 

ABSTRACT

The rhizomes, stems and leaves of Etlingera sayapensis were extracted with ethyl acetate, acetone, ethanol and methanol to give 12 extracts. The total phenolic content (TPC) of the extracts was measured in this study, together with their antioxidant activity. Assays used were radical scavenging activity (RSA), β-carotene bleaching (BCB), ferrous ion chelating (FIC) and ferric reducing antioxidant power (FRAP). Among the assays, the leaf methanolic extract showed the highest activity with 35.67 mg GAE/g (mg of gallic acid per gram of extract) for TPC, IC50 of 53.43 μg/mL for RSA, 83.24% for BCB, IC50 of 242.43 μg/mL for FIC (second highest) and 19.53 mg TrE/g (mg of Trolox per gram of extract) for FRAP. The measured BCB values for the four leaf extracts were higher than those of the positive controls of butylated hydroxytoluene (BHT), gallic acid (GA) and ascorbic acid (AscA) and slightly lower than that of α-tocopherol (Toc). The antimicrobial activity of the extracts was also measured using disc-diffusion (DD) and minimum inhibition concentration (MIC) assays. Among the extracts, only ethanolic and methanolic extracts showed specific bacterial inhibition against Bacillus subtilis. Only stem methanolic extract exhibited specific fungal inhibition against Candida parapsilosis. The highest activity was shown by this with 9.9 mm and 1.04 mg/mL for DD and MIC assays respectively.

 

Keywords: Antimicrobial activity; antioxidant activity; Etlingera; Zingiberaceae

 

ABSTRAK

Rizom, batang dan daun Etlingera sayapensis telah diekstrak dengan etil asetat, aseton, etanol dan metanol untuk memberikan 12 ekstrak. Kandungan fenolik jumlah (TPC) ekstrak telah diukur dalam kajian ini, bersama-sama dengan aktiviti antioksida mereka. Asai yang digunakan adalah aktiviti penskavenjing radikal (RSA), pelunturan β-karotena (BCB), pengkelatan ion ferus (FIC) dan kuasa antioksida penurunan ferik (FRAP). Dalam kalangan asai ini, ekstrak metanol daun menunjukkan aktiviti tertinggi dengan 35.67 mg GAE/g (mg asid galik per gram ekstrak) bagi TPC, IC50 53.43 μg/mL bagi RSA, 83.24% bagi BCB, IC50 242.43 μg/mL bagi FIC (kedua tertinggi) dan 19.53 mg TrE/g (mg Troloks per gram ekstrak) bagi FRAP. Nilai BCB yang diukur bagi empat ekstrak daun adalah lebih tinggi daripada nilai kawalan positif hidroksitoluena terbutil (BHT), asid galik (GA), asid askorbik (AscA) dan rendah sedikit daripada nilai bagi α-tokoferol (Toc). Aktiviti antimikrob ekstrak juga telah diukur menggunakan asai peresapan-cakera (DD) dan kepekatan perencatan minimum (MIC). Dalam kalangan ekstrak, hanya ekstrak etanol dan metanol menunjukkan perencatan bakteria yang spesifik melawan Bacillus subtilis. Hanya ekstrak metanol batang memperlihatkan perencatan fungus yang spesifik menentang Candida parapsilosis. Aktiviti tertinggi telah ditunjukkan olehnya dengan 9.9 mm dan 1.04 mg/mL bagi asai DD dan MIC.

 

Kata kunci: Aktiviti antimikrob; aktiviti antioksida; Etlingera; Zingiberaceae

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*Pengarang untuk surat-menyurat; email: wanyaa@ukm.my

 

 

 

 

 

 

 

 

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