Sains Malaysiana 49(6)(2020): 1303-1312

http://dx.doi.org/10.17576/jsm-2020-4906-08

 

Anti-inflammatory Effect of Tamarind Seed Coat Extract against LPS-Induced RAW264.7 Macrophages
(Kesan Anti-Keradangan Ekstrak Lapisan Biji Asam Jawa terhadap Penggalak LPS RAW264.7 Makrofaj)

 

JIRAPA PUNTARUT1, WIPAWADEE SIANGLUM2, SUPITA TANASAWET3, PENNAPA CHONPATHOMPIKUNLERT4 & WANIDA SUKKETSIRI1*

 

1Department of Pharmacology, Faculty of Science, Prince of Songkla University, Songkhla, 90110 Thailand

 

2Department of Microbiology, Faculty of Science, Prince of Songkla University, Songkhla, 90110

Thailand

 

3Department of Anatomy, Faculty of Science, Prince of Songkla University, Songkhla, 90110, Thailand

 

4Expert Center of Innovative Health Food, Thailand Institute of Scientific and Technological Research (TISTR), Pathumthani, 12120, Thailand

 

Diserahkan: 2 Ogos 2019/ Diterima: 17 Februari 2020

 

ABSTRACT

Inflammatory response is modulated by stimulated immune cells, and has a pivotal role in host defense system against various stimuli. In this study, we evaluated the anti-inflammatory property of tamarind seed coat extract (TSCE) in lipopolysaccharide (LPS)-induced RAW264.7 macrophages. Various concentrations of TSCE (10, 25, and 50 µg mL-1) were applied and then stimulated with LPS (1 μg mL-1) in RAW264.7 macrophages and the level of reactive oxygen species (ROS) and nitric oxide (NO) were measured. Besides, enzyme-linked immunosorbent assay (ELISA) was used to measure the level of pro-inflammatory cytokines. Our results showed that TSCE suppressed LPS-induced intracellular ROS production and suppressed the NO levels in a dose-dependent manner. Significantly, the anti-inflammatory activity was correlated with a lowered LPS-stimulated TNF-α and IL-1β pro-inflammatory cytokines. These results implied that TSCE possess potent anti-inflammatory activity, which supported new insights into the TSCE utilization to protect inflammation-related disorders.

 

Keywords: Interleukin-1β; reactive oxygen species; Tamarindus indica; tumor necrosis factor alpha

 

ABSTRAK

Tindak balas keradangan dimodulasi oleh sel-sel imun yang dirangsang dan memainkan peranan penting dalam sistem pertahanan perumah terhadap pelbagai rangsangan. Dalam kajian ini, kami menilai sifat anti-radang daripada ekstrak lapisan biji asam jawa (TSCE) pada lipopolisakarida (LPS) yang diaruh makrofaj RAW264.7. Pelbagai kepekatan TSCE (10, 25 dan 50 µg mL-1) digunakan dan kemudian dirangsang dengan LPS (1 μg mL-1) pada makrofaj RAW264.7 dan tahap spesies oksigen reaktif (ROS) dan nitrik oksida (NO) diukur. Selain itu, asai imunosorben berkait enzim (ELISA) digunakan untuk mengukur tahap sitokin pro-radang. Hasil kajian menunjukkan bahawa TSCE menindas pengeluaran intraselular ROS yang disebabkan oleh LPS serta menindas aras NO bergantung pada dos atau kepekatan. Aktiviti anti-radang berkorelasi dengan penurunan LPS yang merangsang sitokin pro-radang TNF-α dan IL-1β secara ketara. Hasil ini menunujukkan bahawa TSCE memiliki aktiviti anti-radang yang tinggi sekaligus menyokong pandangan baru tentang penggunaan TSCE dalam melindungi gangguan yang berkaitan dengan keradangan.

 

Kata kunci: Faktor nekrosis tumor alfa; interleukin-1β; spesies oksigen reaktif; Tamarindus indica

 

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*Pengarang untuk surat-menyurat; email: wanida.su@psu.ac.th

 

 

 

 

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