Sains Malaysiana 50(1)(2021): 123-133

http://dx.doi.org/10.17576/jsm-2021-5001-13

Micro-Solid Phase Extraction of Polycyclic Aromatic Hydrocarbons in Water using either C₁₈ or Molecularly Imprinted Polymer Membranes: Analytical Merits and Limitations

 (Pengekstrakan Fasa Pepejal-Mikro bagi Hidrokarbon Aromatik Polisiklik dalam Air Menggunakan Sama Ada Membran C₁₈ atau Polimer Molekul Teraan: Kebaikan dan Kelemahan Analisis)

SITI NURUL UMIRA MOHD SABARI¹, SAW HONG LOH¹*, SAZLINDA KAMARUZAMAN², NOORFATIMAH YAHAYA³ & WAN MOHD AFIQ WAN MOHD KHALIK¹

¹Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu Darul Iman, Malaysia

²Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia

³Integrative Medicine Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, 13200 Bertam Kepala Batas, Pulau Pinang, Malaysia

Diserahkan: 22 Mac 2020/Diterima: 1 Julai 2020

ABSTRACT

Sample pre-treatment is often the bottleneck in an analytical process. Due to the drawbacks of conventional sample pre-treatment methods, microextraction utilizing lower amounts of adsorbents and organic solvents are therefore favoured. A micro-solid phase extraction (μ-SPE) technique coupled with gas chromatography-flame ionization detection (GC-FID) was successfully developed for the analysis of selected polycyclic aromatic hydrocarbons (PAHs), namely phenanthrene, fluoranthene, and pyrene, in environmental water. In this study, μ-SPE techniques using C₁₈ and molecularly imprinted polymer (MIP) membranes were optimized, validated, and applied to the analysis of selected PAHs in environmental water samples. The analytical merits were compared, and the two methods were evaluated in terms of linearity, repeatability, and relative recovery. Under the optimal extraction conditions, both μ-SPE techniques using either C₁₈ or MIP membranes as the adsorbents offered comparable ultratrace analysis of the selected PAHs in the range of 0.003 to 0.01 µg L^–1. The extraction strength of C₁₈ membranes was superior to that of MIP membranes for the extraction of low molecular weights PAHs from water in the presence of humic acid as a matrix factor. The C₁₈ membranes overcome the non-covalence interaction between PAHs and humic acid and thus achieve better recovery.

Keywords: C₁₈; humic acid; micro-solid phase extraction; MIP; polycyclic aromatic hydrocarbons

ABSTRAK

Pra-rawatan sampel selalu menjadi halangan dalam satu proses analisis. Disebabkan kelemahan yang timbul dalam kaedah pra-rawatan sampel yang konvensional, mikro pengekstrakan yang menggunakan amaun penjerap dan pelarut organik yang lebih rendah adalah lebih disukai. Satu teknik pengekstrakan fasa mikro pepejal (μ-SPE) bergabungan kromatografi gas-pengesanan pengionan nyala (GC-FID) telah berjaya dibangunkan untuk analisis hidrokarbon aromatik polisiklik (PAHs) terpilih, iaitu fenantrena, fluorantena dan pirena, dalam air alam sekitar. Dalam kajian ini, teknik μ-SPE menggunakan C₁₈ dan polimer molekul teraan telah dioptimum, divalidasi dan diaplikasi dalam analisis PAHs terpilih dalam sampel air alam sekitar. Kebaikan analitikal dibandingkan dan kedua-dua teknik dinilai daripada segi kelinearan, kebolehulangan dan perolehan semula secara relatif. Di bawah keadaan pengekstrakan yang optimum, kedua-dua teknik μ-SPE yang menggunakan sama ada membran C₁₈ atau MIP sebagai penjerap menawarkan analisis ultra-surih PAHs terpilih yang setanding dalam lingkungan 0.003 hingga 0.01 µg L^–1. Kekuatan mengekstrak membran C₁₈adalah terunggul jika dibandingkan dengan membran MIP khususnya dalam mengektrak PAHs berjisim molekul rendah daripada air dengan kehadiran asik humik sebagai satu faktor matriks. Membran C₁₈ mengatasi interaksi bukan kovalen yang wujud antara PAHs dan asik humik dan seterusnya mencapai perolehan semula yang lebih baik.

Kata kunci: Asid humik; C₁₈; hidrokarbon aromatik polisiklik; MIP; pengekstrakan fasa mikro pepejal

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*Pengarang untuk surat-menyurat; email: lohsh@umt.edu.my