Sains Malaysiana 50(3)(2021): 723-733


Artificial Cultivation Anti-tumor Activity of Ganoderma mbrekobenum

(Penanaman Buatan dan Aktiviti Anti-barah Ganoderma mbrekobenum)




1Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangdong 510070, Guangzhou, China


2Guangdong Yuewei Edible Fungi Technol. Co. Ltd., Guangdong 510663, Guangzhou, China


Diserahkan: 25 Disember 2019/Diterima: 26 Ogos 2020



Different varieties of Gandoerma have different functional effects. In this study, identification, cultivation, anti-tumor activity characterization and active constituent determination of Ganoderma specimen collected in Tanzania were carried out. The fungi specimen was identified as Ganoderma mbrekobenum by morphological and molecular methods, which was found in Ghana and was firstly reported in 2016. The fruit body was formed in artificial culture using sawdust as the main substrate at the suitable fruiting temperature of 27-30 ℃, relative humidity 85-90%, and daily processed lighting for 10 h (300-500 Lux). The fruit body of G. mbrekobenum is hard and has ordinary-yield production, uniform shapes, and high stability. The in vitro experiments showed that the extract from G. mbrekobenum fruit body inhibited cancer cell proliferation of various cells, such as HepG2, MDA-MB-231 and U87. The water extract of the fruit body was tested, and the content of crude polysaccharides was 1.12%. The data showed that G. mbrekobenum was a potential valuable variety. This is the first study that reports the cultivation method and the anti-tumor activity of G. mbrekobenum.


Keywords: Ganoderma; medical fungi; polysaccharides



Varieti Ganoderma yang berbeza mempunyai kesan kefungsian yang berbeza. Dalam kajian ini, pengenalpastian, penanaman, pencirian aktiviti anti-barah dan penentuan sebatian aktif terhadap spesimen Ganoderma yang diperoleh dari Tanzania telah dijalankan. Melalui kaedah morfologi dan molekul, spesimen kulat tersebut telah dikenal pasti sebagai Ganoderma mbrekobenum, yang sebelum ini ditemui di Ghana dan pertama kali dilaporkan pada tahun 2016. Jasad buah telah terhasil dalam kultur buatan menggunakan serbuk gergaji sebagai substrat utama pada suhu untuk berbuah yang sesuai antara 27-30 °C, kelembapan relatif 85-90% dan pencahayaan setiap hari selama 10 jam (300-500 Lux). Jasad buah G. mbrekobenum adalah keras dan mempunyai penghasilan biasa, berbentuk seragam dan kestabilan yang tinggi. Uji kaji secara in vitro menunjukkan bahawa ekstrak daripada jasad buah G. mbrekobenum merencat pembahagian beberapa sel barah seperti HepG2, MDA-MB-231 dan U87. Ekstrak air jasad buah telah diuji dan didapati bahawa kandungan polisakarida mentah adalah sebanyak 1.12%. Data menunjukkan bahawa kulat ini merupakan varieti bernilai yang berpotensi. Ini merupakan kajian yang pertama melaporkan kaedah penanaman dan aktiviti anti-barah G. mbrekobenum.


Kata kunci: Ganoderma; kulat perubatan; polisakarida



Bao, H. & Wang, X. 2014. Studies on chemical constituents of Ganoderma sinense. Journal of Fungal Research 12(4): 187-196.

Cao, Y., Wu, S.H. & Dai, Y.C. 2012. Species clarification of the prize medicinal Ganoderma mushroom “Lingzhi”. Fungal Diversity 56(1): 49-62.

Cao, Y. & Yuan, H. 2013. Ganoderma mutabile sp. nov. from southwestern China based on morphological and molecular data. Mycological Progress 12(1): 121-126.

Chinese Pharmacopoeia Committee. 2015. Chinese Pharmacopoeia. Beijing: People’s Press.

Coetzee, M.P.A., Marincowitz, S., Muthelo, V.G. & Wingfield, M.J. 2015. Ganoderma species, including new taxa associated with root rot of the iconic Jacaranda mimosifolia in Pretoria, South Africa. IMA Fungus 6(1): 249-256.

Crous, P.W., Wingfield, M.J., Richardson, D.M., Le Roux, J.J., Strasberg, D., Edwards, J., Roets, F., Hubka, V., Taylor, P.W., Heykoop, M., Martín, M.P., Moreno, G., Sutton, D.A., Wiederhold, N.P., Barnes, C.W., Carlavilla, J.R., Gené, J., Giraldo, A., Guarnaccia, V., Guarro, J., Hernández-Restrepo, M., Kolařík, M., Manjón, J.L., Pascoe, I.G., Popov, E.S., Sandoval-Denis, M., Woudenberg, J.H., Acharya, K., Alexandrova, A.V., Alvarado, P., Barbosa, R.N., Baseia, I.G., Blanchette, R.A., Boekhout, T., Burgess, T.I., Cano-Lira, J.F., Čmoková, A., Dimitrov, R.A., Dyakov, M.Y., Dueñas, M., Dutta, A.K., Esteve-Raventós, F., Fedosova, A.G., Fournier, J., Gamboa, P., Gouliamova, D.E., Grebenc, T., Groenewald, M., Hanse, B., Hardy, G.E., Held, B.W., Jurjević, Ž., Kaewgrajang, T., Latha, K.P., Lombard, L., Luangsa-Ard, J.J., Lysková, P., Mallátová, N., Manimohan, P., Miller, A.N., Mirabolfathy, M., Morozova, O.V., Obodai, M., Oliveira, N.T., Ordóñez, M.E., Otto, E.C., Paloi, S., Peterson, S.W., Phosri, C., Roux, J., Salazar, W.A., Sánchez, A., Sarria, G.A., Shin, H.D., Silva, B.D., Silva, G.A., Smith, M.T., Souza-Motta, C.M., Stchigel, A.M., Stoilova-Disheva, M.M., Sulzbacher, M.A., Telleria, M.T., Toapanta, C., Traba, J.M., Valenzuela-Lopez, N., Watling, R. & Groenewald, J.Z. 2016. Fungal planet description sheets: 400-468. Persoonia- Molecular Phylogeny and Ecolution of Fungi 36(1): 316-458.

Costa-Rezende, D.H., Gugliotta, A.D., Mello, Goes-Neto, A., Reck, M.A., Robledo, G.L. & Drechsler-Santos, E.R. 2016. Amauroderma calcitum sp. nov. and notes on taxonomy and distribution of Amauroderma species (Ganodermataceae). Phytotaxa 244(2): 101-124.

Elliott, M.L., Des, J., Elizabeth, A., Ortiz, J.V. & Macias, T. 2018. Genetic variability of Ganoderma zonatum infecting palms in Florida. Mycologia 110(2): 339-346.

Hsu, K. & Cheng, K. 2018. From nutraceutical to clinical trial: Frontiers in Ganoderma development. Applied Microbiology & Biotechnology 102(21): 9037-9051.

Hu, H.P., Liu, Y.C., Mo, W.P., Huang, L.H., Zhang, Y.F., Li, T.H., Chen, R. & Xie, Y.Z. 2017. Isolation, characterization and anti-cancer activity of two Ganoderma leucocontextum srains. Acta Edulis Fungi 24(1): 50-54.

Hu, H.P., Zhang, Y.F., Zhang, Z., Shao, M.C., Wu, Q.P., Keto, M. & Yang, X.B. 2011. Bioactive components in cultivated fruit bodies of three wild Ganoderma strains collected from Tanzania. Acta Edulis Fungi 18(3): 64-66.

Isaac, I.L., Yin, W.A.W.C., Bakar, M.F.A., Idris, A.S., Bakar, F.D.A., Bharudin, I. & Murad, A.M.A. 2018. Transcriptome datasets of oil palm pathogen Ganoderma boninense. Data in Brief 17: 1108-1111.

Jia, H.Y., Wang, Y.T., Zhang, Z.H., Feng, N., Liu, Y.F., Zhou, S.Z., Zhang, Z., Zhang, J.S. & Tang, Q.J. 2017. Determination of triterpenoids in Ganodema lingzhi from different areas and species by HPLC. Microbiology China 44(1): 238-244.

Kubota, T., Yukihiro, A., Iwao, M. & Hideo, M. 1982. Structures of ganoderic acid A and B, two new lanostane type bitter triterpenes from Ganoderma lucidum (FR.) KARST Helvetica Chimica Acta 65(2): 611-619.

Li, P., Deng, Y., Wei, X. & Xu, J. 2013. Triterpenoids from Ganoderma lucidum and their cytotoxic activities. Natural Product Research 27(1): 17-22.

Li, T., Hu, H., Deng, W., Wu, S., Wang, D. & Tamdrin, T. 2015a. Ganoderma leucocontextum, a new member of the G. lucidum complex from southwestern China. Mycoscience 56(1): 81-85.

Li, Y., Li, T.H., Yang, Z.L., Bau, T. & Dai, Y.C. 2015b. Atlas of Chinese Macrofungal Resources. Zhengzhou: Zhongyuan People's Publishing House. pp. 401-412.

Li, X.M., Xie, Y.Z., Peng, J.J., Hu, H.P., Wu, Q.P. & Yang, B.B. 2019. Ganoderiol F purified from Ganoderma leucocontextum retards cell cycle progression by inhibiting CDK4/CDK6. Cell Cycle 18(21): 1-14.

Lin, Z.B. 2001. The integrated studies on Ganoderma lucidum Kayst. guided by traditional Chinese medical theories. Chinese Journal of Integrated Traditional Western Medicine 21(12): 883-884.

Liu, W., Tang, Q.J., Zhang, G.Y., Feng, N. & Han, W. 2018. In vitro anti-tumor and immunological activity of Ganoderma lobatum. Microbiol China. 45(4): 819-824.

Liu, Y.C., Liu, P., Hu, H.P., Li, D. & Li, Y. 2016. Tricholosporum, a newly recorded genus of Agaricomycetes in China. Phytotaxa. 289(3): 263-270.

Luangharn, T., Karunarathna, S.C., Khan, S., Xu, J., Mortimer, P. & Hyde, K. 2017. Antibacterial activity, optimal culture conditions and cultivation of the medicinal Ganoderma australe, new to Thailand. Mycosphere 8(8): 1108-1123.

Mehmet, S.D., Mehmet, C.Y., Ayşe, G., İzzet, Y. & Akdag, M.Z. 2017. The protective effect of melatonin and Ganoderma lucidum against the negative effects of extremely low frequency electric and magnetic fields on pulp structure in rat teeth. Biotechnology & Biotechnological Equipment 31(5): 979-988.

Osińska-Jaroszuk, M., Jaszek, M., Mizerska-Dudka, M., Błachowicz, A., Rejczak, T.P., Janusz, G., Wydrych, J., Polak, J., Jarosz-Wilkołazka, A. & Kandefer-Szerszeń, M. 2014. Exopolysaccharide from Ganoderma applanatum as a promising bioactive compound with cytostatic and antibacterial properties. BioMed Research International 2014(1): 743812-743812.

Raseta, M., Popovic, M., Capo, I., Stilinovic, N., Vukmirovic, S., Milosevic, B. & Karaman, M. 2020. Antidiabetic effect of two different Ganoderma species tested in alloxan diabetic rats. RSC Advances 10(17): 10382-10393.

Saccardo, P.A. & Saccardo, D. 1905. Supplementum universale. Pars VI. Hymenomycetae-Laboulbeniomycetae. Sylloge Fungorum 17: 860.

Sliva, D., Loganthan, J., Jiang, J., Jedinak, A., Lamb, J.G., Terry, C., Baldridge, L.A., Adamec, J., Sandusly, G.E. & Dudhgaonkar, S. 2012. Mushroom Ganoderma lucidum prevents colitis-associated carcinogenesis in mice. PLoS ONE 7(10): 1-13.

Syahriel, A., Yee, S.L., Sylvia, J.D., Arnnyitte, A. & Khim, P.C. 2018. Ganoderma boninense isolated from Sabah, Malaysia exhibits potent antibacterial activity against clinically important bacterial pathogens. Bangladesh Journal of Pharmacology 13(1): 10-12.

Wang, D.M., Wu, S.H., Su, C.H., Peng, J.T., Shi, Y.H. & Chen, L.Z. 2009. Ganoderma multipileum, the correct name for 'G. lucidum' in tropical Asia. Botanical Studies 50(4): 451-458.

White, T.J., Bruns, T.D., Lee, S. & Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols A Guide to Methods & Applications, edited by Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. New York: Academic Press, Inc. pp. 315-322.

Wu, Q.P., Xie, Y.Z., Deng, Z.Q., Li, X.M., Yang, W.N., Jiao, C.W., Ling, F., Li, S.Z., Pan, H.H. & Yee, A.J. 2012. Ergosterol peroxide isolated from Ganoderma lucidum abolishes microRNA miR-378-mediated tumor cells on chemoresistance. PLoS ONE 7(8): 1-14.

Xing, J.H. & Cui, B.K. 2018. Ganoderma species diversity, classification and phylogenetic studies, Chinese Society of Fungus Academic Conference in Shandong Taian, China. p. 295.

Xing, J.H., Sun, Y.F., Han, Y.L., Cui, B.K. & Dai, Y.C. 2018. Morphological and molecular identification of two new Ganoderma species on Casuarina equisetifolia from China. Mycokeys 34: 93-108.

Xing, J.H., Song, J., Decock, C. & Cui, B.K. 2016. Morphological characters and phylogenetic analysis reveal a new species within the Ganoderma lucidum complex from South Africa. Phytotaxa 266(2): 115-124.

Xing, Z.T., Men, D.Y., Tang, Q.J., Wang, N., Li, M.R. & Guan, S.M. 2008. NY/T 1676-2008 Determination of Crude Mushroom Polysaccharides. The Ministry of Agriculture of the People’s Republic of China. Beijing: China Agriculture Press.

Yong, T.Q., Chen, S.D., Xie, Y.Z., Chen, D.L., Su, J.Y., Shuai, O., Jiao, C.W. & Zuo, D. 2018. Hypouricemic effects of Ganoderma applanatum in hyperuricemia mice through OAT1 and GLUT9. Frontiers in Pharmacology 8(996): 1-11.

Zhao, J.D. & Zhang, X.Q. 2000. Flora Fungorum Sinicorum Vol. 18 Ganodermataceae. Beijing: Science Press.

Zhou, L.W., Cao, Y., Wu, S.H., Josef, V., Li, D.W., Li, M.J. & Dai, Y.C. 2014. Global diversity of the Ganoderma lucidum complex (Ganodermataceae, Polyporales) inferred from morphology and multilocus phylogeny. Phytochemistry 114: 7-15.


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