Sains Malaysiana 50(5)(2021): 1407-1414


Pterostilbene Supplementation Inhibits Early Inflammatory Response and Oxidative Stress in UVB-Induced BALB/C Mice

(Suplementasi Pterostilbene Merencat Tindak Balas Keradangan Awal dan Tekanan Oksidatif pada Tikus BALB/C yang Diaruh-UVB)




1Biomedical Science Programme, Centre for Toxicology and Health Risk Studies, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Federal Territory, Malaysia


2Biomedical Science Programme, Centre for Diagnostic, Therapeutic and Investigations, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Federal Territory, Malaysia


Diserahkan: 6 Januari 2020/Diterima: 29 September 2020



Extended dermal exposure of ultraviolet B (UVB) can induce erythema, hyperpigmentation, epidermal hyperplasia and cancer. Natural active compound such as pterostilbene (PS) is a potential UV-protecting agent as it has broad biological activities. This study aimed to evaluate the photoprotective effect of pterostilbene on ultraviolet-B-induced BALB/c mice. Twenty-four female mice were randomised into four groups (n=6/group): vehicle control (VC); UVB irradiated only (UVB only); UVB irradiation treated with pterostilbene 10 mg/kg (PS10+UVB); (iv) UVB irradiation treated with pterostilbene 20 mg/kg (PS20+UVB). The PS treatments were given for 14 days, and UVB was given at dose 250 mJ/cm2 on days 9, 11, and 13 of the treatment periods. The results showed that PS lessened redness and scaling on the skin of UVB-irradiated mice. The skinfold thickness and epidermal thickness in the PS-treated group were significantly reduced (p<0.05) in comparison with those in the UVB only group. The PS10 and PS20 groups (5.927 ± 0.354 and 5.660 ± 0.765 nmol/g, respectively) demonstrated significantly decreased MDA levels (P<0.05) relative to the UVB only group (13.343 ± 1.350 nmol/g). The GSH level in both PS10 (0.555 ± 0.020 µmol/mg) and PS20 (0.568 ± 0.055 µmol/mg) groups increased significantly (p<0.05) compared with that in the UVB only group (0.376 ± 0.025 µmol/mg). SOD activity in the PS20 group (1.388 ± 0.172 U/min/mg) increased significantly (p<0.05) compared with that in the UVB only group (0.561 ± 0.034 U/min/mg). Histological observation showed that PS reduced leukocyte infiltration and epidermal hyperplasia. Hence, oral PS may exert a photoprotective effect by acting as an anti-inflammatory and antioxidant agent on UVB-irradiated mice skin.


Keywords: Inflammation; oxidative stress; photoprotection; pterostilbene; UVB



Pendedahan kulit terhadap cahaya ultraungu B (UVB) secara berpanjangan boleh menyebabkan eritema, hiper-pigmentasi, hiperplasia epidermis dan kanser. Sebatian aktif semula jadi seperti pterostilbene (PS) berpotensi untuk digunakan sebagai agen perlindungan UV kerana ia mempunyai aktiviti biologi yang luas. Kajian ini bertujuan untuk menilai kesan pemfotolindungan pterostilbene terhadap tikus yang diaruh sinaran UVB. Dua puluh empat ekor tikus betina dibahagikan kepada empat kumpulan secara rawak: kumpulan kawalan negatif (n=6), tanpa sinaran UVB dan rawatan; disinarkan UVB sahaja (n=6); disinarkan UVB dan dirawat dengan pterostilbene 10 mg/kg (PS10+UVB); disinarkan UVB dan dirawat dengan pterostilbene 20 mg/kg (PS20+UVB). Rawatan PS diberikan selama 14 hari, manakala pendedahan kepada UVB diberikan dengan dos 250 mJ/cm2 pada hari ke-9, -11, dan -13 dalam tempoh rawatan. PS telah mengurangkan kulit bersisik dan eritema pada tikus aruhan UVB. PS telah mengurangkan ketebalan lipatan kulit tikus secara signifikan (p <0.05) berbanding dengan kumpulan tikus yang disinarkan UVB sahaja. Ketebalan epidermis juga menurun dengan signifikan (p<0.05) berbanding dengan kumpulan UVB sahaja. Kumpulan PS10 (5.927±0.354 nmol/g) dan PS20 (5.660±0.765 nmol/g) telah menunjukkan penurunan paras MDA yang signifikan (p<0.05) berbanding kumpulan tikus yang disinarkan UVB sahaja (13.343 ± 1.350 nmol/g). Aras GSH bagi kumpulan PS10 (0.555±0.02 μmol/mol) dan PS20 (0.568 ± 0.055 μmol/mol) telah meningkat secara signifikan (p<0.05) berbanding dengan kumpulan tikus yang disinarkan UVB sahaja (0.376±0.026 μmol/mol). Kumpulan PS20 (1.388±0.171 U/min/mg) telah menunjukkan peningkatan aktiviti SOD secara signifikan (p<0.05) berbanding dengan kumpulan tikus yang disinarkan UVB sahaja (0.561±0.034 U/min/mg). Pemerhatian histologi menunjukkan bahawa PS mengurangkan penyusupan leukosit dan hiperplasia epidermis. Oleh itu, PS oral boleh memberikan kesan pemfotolindungan dengan bertindak sebagai agen anti-radang dan antioksidan pada kulit tikus yang disinarkan UVB.


Kata kunci: Keradangan; pemfotolindungan; pterostilbene; tekanan oksidatif; UVB



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