Sains Malaysiana 51(5)(2022): 1339-1352

http://doi.org/10.17576/jsm-2022-5105-06

Water Quality and Microbial Community Assessment in Artisanal Mining- Affected Sediments of Cikidang River, Banten, Java, Indonesia 

(Penilaian Kualiti Air dan Komuniti Mikrob dalam Perlombongan Artisanal- Sedimen Terjejas Sungai Cikidang, Banten, Java, Indonesia)

NOVERITA DIAN TAKARINA^1*, AYU MAULIDA SUKMA² , ANDRIO ADIWIBOWO³ & SITARESMI¹

¹Department of Biology, Faculty of Mathematics and Natural Sciences University of Indonesia, 16424 Beji Depok, West Java, Indonesia

²Biology Post Graduate Program, Department of Biology, Faculty of Mathematics and Natural Sciences University of Indonesia, 16424 Beji Depok, West Java, Indonesia

³Faculty of Public Health University of Indonesia, 16424 Beji Depok, West Java Indonesia

Diserahkan: 5 Disember 2020/Diterima: 22 September 2021

Abstract

The total microbial diversity and community in submerged sediments near mining sites, transition and intact sites at Cikidang River, Banten were assessed using 16S rDNA sequence data and biodiversity indices. Assessed water quality parameters in the river were water current, dissolved oxygen (DO), pH, redox potential, salinity, temperature and turbidity. Microbial alpha diversity used were Shannon diversity index, ChaoI index and Operational Taxonomic Units (OTUs). These analyses indicated a total of 50 taxa of sediment microbes. Based on Shannon diversity index, the highest value was observed at mining site. High abundant microbes in sediments at mining sites for phylum, class, order, family and genera levels were represented by Proteobacteria, Gammaproteobacteria, Pseudomonadales, Moraxellaceae, and Acinetobacter, respectively. In contrast, high abundant microbes in the sediment of the intact site for each taxon consisted of Firmicutes, Alphaproteobacteria, Erysipelotrichales, Erysipelotrichaceae, and Erysipelothrix. The water quality of mining sites was characterised by alkaline pH (8.807, 95% CI: 8.624 - 8.990) and lower redox potential (59.000 mV, 95% CI: 36.233 - 81.767) that differed significantly from the intact sites (P<0.05). This study also confirmed that mining sites have a higher genus diversity. Research on potential microbes of related genus as bioremediators could be recommended for further study.

Keywords: Community; microorganism; mining; sediment; 16S rDNA sequence data

Abstrak

Seluruh kepelbagaian dan komuniti mikrob dalam sedimen terendam berdekatan dengan tapak perlombongan, peralihan dan lokasi utuh di Sungai Cikidang, Banten telah dinilai menggunakan data jujukan 16S rDNA dan indeks kepelbagaian biologi. Parameter kualiti air yang dinilai di sungai termasuk arus air, oksigen terlarut (DO), pH, potensi redoks, saliniti, suhu dan kekeruhan. Kepelbagaian alfa mikrob yang digunakan adalah indeks kepelbagaian Shannon, indeks ChaoI dan Unit Taksonomi Operasi (OTU). Analisis ini menunjukkan sejumlah 50 taksa mikrob sedimen. Berdasarkan indeks kepelbagaian Shannon, nilai tertinggi diperhatikan di tapak perlombongan. Kelimpahan mikrob yang tinggi dalam sedimen di tapak perlombongan bagi tahap filum, kelas, order, famili dan genus masing-masing diwakili oleh Proteobacteria, Gammaproteobacteria, Pseudomonadales, Moraxellaceae dan Acinetobacter. Sebaliknya, mikrob yang banyak terdapat dalam sedimen di tapak utuh bagi setiap takson terdiri daripada Firmicutes, Alphaproteobacteria, Erysipelotrichales, Erysipelotrichaceae dan Erysipelothrix. Kualiti air di tapak perlombongan dicirikan oleh pH alkali (8.807, 95% CI: 8.624-8.990) dan potensi redoks rendah (59.000 mV, 95% CI:36.233-81.767) yang sangat berbeza secara signifikan daripada tapak utuh (P<0.05). Kajian ini juga mengesahkan bahawa tapak perlombongan mempunyai kepelbagaian genus yang lebih tinggi. Penyelidikan mengenai mikrob yang berpotensi daripada genus yang berkaitan sebagai bioremediasi boleh disarankan untuk kajian lanjutan.

Kata kunci: Data jujukan 16S rDNA; komuniti; mikroorganisma; perlombongan; sedimen

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^*Pengarang untuk surat-menyurat; email: noverita.dian@sci.ui.ac.id