Sains Malaysiana 51(7)(2022): 2137-2146

http://doi.org/10.17576/jsm-2022-5107-16

 

Combined Antioxidant Formulation of Ascorbic Acid with Resveratrol Ameliorates Isoproterenol-Induced Myocardial Infarction in Rats

(Gabungan Formulasi Antioksidan Asid Askorbik dengan Resveratrol yang Ditambah Baik dengan Isoproterenol-Penginfarkan Miokardium Teraruh dalam Tikus)

 

HAICHAO GAO1, HONG WANG1,*, TINGTING HAN2, XIANSHENG HUANG1, SHUCHENG LI1 & XIBING WANG1

 

1Department of Cardiology, Affiliated Hospital of Chengde Medical College, No 36,  Nanyingzi Street 067000, Chengde, Hebei Province, China

2Department of Emergency, The Third Central Hospital of Tianjin, 300170 Tianjin, China

 

Diserahkan: 31 Julai 2021/Diterima: 7 Disember 2021

 

ABSTRACT

­­The current investigation was proposed to assess the effectiveness of the combination of resveratrol and ascorbic acid against Isoproterenol (ISO)-induced myocardial infarction in rats.  The experimental model was divided into six groups (n = 6 in each group). Group I: control, Group 2: isoproterenol (ISO)-100 mg/kg b.wt, Group 3: ISO+Resveratrol (RES) (20 mg/kg b.wt) treated, Group 4: ISO+ Ascorbic acid (AA) (80 mg/kg b.wt) treated, Group 5: ISO+RES (20 mg/kg b.wt)+AA (80 mg/kg b.wt) treated and  Group 6: RES (20 mg/kg b.wt)+ AA (80 mg/kg b.wt) alone treated. The study showed an increase in lipid peroxides and cardiac markers in the serum samples of experimental animals administered with ISO. Treatment with RES and AA individually and a combinational formulation brought a significant decrease in lipid peroxides and cardiac markers. They increased the level of enzymatic antioxidants and non-enzymatic antioxidant levels. Histopathological results showed the distortion of heart architecture among the experimental groups administered with ISO and significant recovery when treated with RES and AA individually and in their combination. The study presents the effective combination of RES and AA in combating ISO-induced myocardial infarction and protection against ROS-mediated oxidative stress.

 

Keywords: Ascorbic acid; isoproterenol; myocardial infarction; resveratrol

 

ABSTRAK

Penyelidikan ini dicadangkan untuk menilai keberkesanan gabungan resveratrol dan asid askorbik terhadap penginfarkan miokardium yang disebabkan oleh Isoproterenol (ISO) pada tikus. Model uji kaji dibahagikan kepada enam kumpulan (n = 6 dalam setiap kumpulan). Kumpulan I: kawalan, Kumpulan 2: isoproterenol (ISO)-100 mg/kg b.wt, Kumpulan 3: ISO+Resveratrol (RES) (20 mg/kg b.wt) terawat, Kumpulan 4: ISO+ Askorbik asid (AA) (80 mg/kg b.wt) terawat, Kumpulan 5: ISO+RES (20 mg/kg b.wt)+AA (80 mg/kg b.wt) terawat dan Kumpulan 6: RES (20 mg/kg b. wt)+ AA (80 mg/kg b.wt) sahaja terawat. Kajian menunjukkan peningkatan dalam peroksida lipid dan penanda jantung dalam sampel serum haiwan uji kaji yang telah diberi ISO. Rawatan dengan RES dan AA secara individu dan gabungan formulasi membawa penurunan yang ketara dalam peroksida lipid dan penanda jantung. Ia meningkatkan tahap antioksidan berenzim dan tahap antioksidan bukan berenzim. Keputusan histopatologi mendedahkan herotan arkitektur jantung dalam kalangan kumpulan uji kaji yang diadministrasi dengan ISO dan pemulihan yang ketara apabila dirawat dengan RES dan AA secara individu dan secara gabungan. Kajian itu membentangkan gabungan RES dan AA yang berkesan dalam memerangi penginfarkan miokardium yang disebabkan oleh ISO dan perlindungan terhadap tekanan oksidatif pengantaraan ROS.

 

Kata kunci: Asid askorbik; isoproterenol; penginfarkan miokardium; resveratrol

 

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*Pengarang untuk surat-menyurat; email: cdwanghong666@sina.com

 

   

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