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Sains Malaysiana 52(1)(2023): 17-33
http://doi.org/10.17576/jsm-2023-5201-02
A Review on
Next-Generation Wildlife Monitoring using Environmental DNA (eDNA) Detection and
Next-Generation Sequencing in Malaysia
(Kajian Pemantauan Hidupan Liar Generasi Masa Hadapan menggunakan Pengesanan DNA (eDNA) Persekitaran dan Penjujukan Generasi Masa Hadapan di Malaysia)
NURSYUHADA
OTHMAN1, KAVIARASU MUNIAN1,2, HIDAYAH HARIS1,
FARAH FARHANA RAMLI1, NUR HARTINI SARIYATI1, MOHD FAUDZIR
NAJMUDDIN1 & MUHAMMAD ABU BAKAR ABDUL-LATIFF1,*
1Environmental
Management and Conservation Research Unit (eNCORe),
Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia (Pagoh Campus), 84600 Muar,
Johor Darul Takzim,
Malaysia
2Zoology Branch, Forest
Biodiversity Division, Forest Research Institute Malaysia, 52109 Kepong,
Selangor Darul Ehsan, Malaysia
Diserahkan: 14 Februari 2022/Diterima: 15 Oktober 2022
Abstract
The use of environmental DNA (eDNA) as the genetic
tool to monitor biodiversity has been increasing across the world, including
Malaysia. Over a decade, the technique has become widely used in wildlife
conservation with the technologies called next-generation sequencing (NGS).
Unfortunately, as one of the top megadiverse countries,
Malaysia is still behind in this field since eDNA methods outperform
traditional surveys. Hence, in this study, we analyzed the paper related to
eDNA studies in Malaysia, which focused on wildlife monitoring. We conducted a
systematic bibliographic search and quantitative review of studies published before
June 2021 from Google Scholar and Scopus database. Forty published eDNA studies
were found, and each paper was classified based on five themes (species
identification, diet assessment, health monitoring, resolve taxonomic,
whole-genome sequencing) according to the study analysis. This study aims to
identify gaps in eDNA in Malaysia, which can later be useful for future
conservation actions and development by applying technology in wildlife
monitoring.
Keywords: Biodiversity; conservation; environmental DNA; eDNA; next-generation sequencing; NGS
Abstrak
Penggunaan DNA persekitaran (eDNA) sebagai alat genetik
untuk memantau kepelbagaian biologi telah pun meningkat di seluruh dunia
termasuk Malaysia. Setelah sedekad, teknik ini telah digunakan secara meluas
dalam pemuliharaan hidupan liar bersama dengan teknologi yang dikenali sebagai
penjujukan generasi masa hadapan (NGS). Walau bagaimanapun, Malaysia yang
merupakan salah sebuah negara di dunia yang mempunyai kekayaan kepelbagaian
biologi yang tinggi masih lagi ketinggalan dalam bidang ini kerana kaedah eDNA
sebenarnya mampu mengatasi kaedah tradisi. Dalam ulasan ini, kami telah
menganalisis kajian yang berkaitan dengan eDNA di Malaysia yang memberi tumpuan
kepada pemantauan hidupan liar. Kami telah menjalankan pencarian secara
sistematik biblografi dan juga secara kuantitatif berdasarkan kajian lepas yang
telah diterbitkan sehingga Jun 2021 menggunakan Google Scholar dan pangkalan
data Scopus. Terdapat 40 kajian eDNA yang telah diterbitkan dan dikelaskan
berdasarkan lima tema (pengenalan spesies, penilaian pemakanan, pemantauan
kesihatan, penyelesaian taksonomi dan penjujukan keseluruhan genom) mengikut
analisis kajian. Tujuan ulasan ini adalah untuk mengenal pasti jurang kajian
eDNA di Malaysia yang dapat membantu untuk aktiviti pemuliharaan dan
pembangunan masa hadapan dengan menggunakan teknologi yang terkini dalam
pemantauan hidupan liar.
Kata kunci: DNA persekitaran; eDNA; kepelbagaian biologi; konservasi; NGS; penjujukan generasi masa hadapan
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*Pengarang untuk surat-menyurat; email: latiff@uthm.edu.my
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