Sains Malaysiana 52(1)(2023): 95-105

http://doi.org/10.17576/jsm-2023-5201-08

 

Effect of Swamp Eel (Monopterus albus) Plasma on The Quality of Tilapia (Oreochromis mossambicus) Surimi

(Kesan Plasma Belut Paya (Monopterus albus) terhadap Kualiti Surimi Tilapia (Oreochromis mossambicus))

 

RODIANA NOPIANTI1,3, EKOWATI CHASANAH2, SUKARNO1 & MAGGY THENAWIDJAJA SUHARTONO1,*

 

1Department of Food Science and Technology, Faculty of Agricultural Technology, Bogor Agricultural University (IPB University), Dramaga, Bogor 16002, Indonesia

2National Agency for Research and Innovation (BRIN)

3Department of Fisheries Product Technology, Faculty of Agriculture, Sriwijaya University, Indralaya, Ogan Ilir, South Sumatera, 30662, Indonesia

 

Diserahkan: 27 Jun 2022/Diterima: 29 September 2022

 

Abstract

The effects of crude plasma and ethanol-extracted of swamp eel plasma at different concentrations (0, 0.25, 0.5, 0.75, 1, 1.5, 2 mg/g) as inhibitor protease in the quality of tilapia (Oreochromis mossambicus) surimi were investigated. The parameters analyzed were gel strength, expressible moisture, whiteness, microstructure, protein solubility, hydrolysis pattern of surimi by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, percent inhibition, and zymogram. The results showed that ethanol-extracted plasma could maintain gel strength, expressible moisture, microstructure, protein solubility, and percent inhibition in lysis of tilapia surimi from protease activity that caused formation of modori structure better than crude plasma. The whiteness decreased as the concentration of plasma used increased. SDS-PAGE for hydrolysis pattern analysis showed that the effectiveness of crude plasma as a protease inhibitor decreased as the concentration increased. There was an increase in the inhibition of protease in the ethanol-extracted plasma at a concentration of 1.5 mg/g. It suggested that the crude plasma contained an internal protease enzyme that might interfere with the reduced myosin intensity.

 

Keywords: Crude plasma; modori; plasma ethanol fraction; swamp eel

 

Abstrak

Kesan plasma mentah belut paya dan diekstrak etanol pada kepekatan berbeza (0, 0.25, 0.5, 0.75, 1, 1.5, 2 mg/g) sebagai perencat protease dalam kualiti surimi tilapia (Oreochromis mossambicus) telah dikaji. Parameter yang dianalisis ialah kekuatan gel, lembapan boleh nyata, keputihan, struktur mikro, keterlarutan protein, corak hidrolisis surimi oleh elektroforesis gel natrium dodesil sulfat-poliakrilamida, perencatan peratus dan zimogram. Keputusan menunjukkan bahawa plasma yang diekstrak etanol boleh mengekalkan kekuatan gel, kelembapan yang boleh diungkapkan, struktur mikro, keterlarutan protein dan peratus perencatan dalam lisis surimi tilapia daripada aktiviti protease yang menyebabkan pembentukan struktur modori lebih baik daripada plasma mentah. Keputihan berkurangan apabila kepekatan plasma yang digunakan meningkat. SDS-PAGE untuk analisis pola hidrolisis menunjukkan bahawa keberkesanan plasma mentah sebagai perencat protease menurun apabila kepekatan meningkat. Terdapat peningkatan dalam perencatan protease dalam plasma yang diekstrak etanol pada kepekatan 1.5 mg/g. Ia mencadangkan bahawa plasma mentah mengandungi enzim protease dalaman yang mungkin mengganggu keamatan miosin yang dikurangkan.

 

Kata kunci: Belut paya; modori; plasma mentah; serpihan etanol plasma

 

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*Pengarang untuk surat-menyurat; email: mthenawidjaja@yahoo.com

 

 

   

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