Sains Malaysiana 52(5)(2023): 1497-1511

http://doi.org/10.17576/jsm-2023-5205-13

 

 

The Impact of Bisphenol A Exposure during Pregnancy on the Heart of Mother and Fetal Rats

(Kesan Pendedahan Bisfenol A kepada Hati Ibu dan Fetus Tikus semasa Bunting)

 

ZATILFARIHIAH RASDI1, ROZIANA KAMALUDIN2, SITI HAMIMAH SHEIKH ABDUL KADIR3,4,*, MOHD DANIAL MOHD EFENDY GOON3,4, JESMINE KHAN3, SHARANIZA AB. RAHIM3 & MOHD HAFIZ DZARFAN OTHMAN2

 

1Centre of Preclinical Sciences Studies, Faculty of Dentistry, Universiti Teknologi MARA, Cawangan Selangor, 47000 Sungai Buloh, Selangor Darul Ehsan, Malaysia

2Advanced Membrane Technology Research Center (AMTEC), School of Chemical and Energy Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor Darul Takzim, Malaysia

3Department of Biochemistry and Molecular Medicine, Faculty of Medicine, Universiti Teknologi MARA, Cawangan Selangor, 47000 Sungai Buloh, Selangor Darul Ehsan, Malaysia

4Institute for Pathology, Laboratory and Forensic Medicine (I-PPerForM), Universiti Teknologi MARA, Cawangan Selangor, 47000 Sungai Buloh, Selangor Darul Ehsan, Malaysia

 

Diserahkan: 6 September 2022/Diterima: 29 Mac 2023

 

Abstract

In utero bisphenol A (BPA) exposure has been reported to increase the risk of cardiovascular disease (CVD) in adult life. Thus, this study aimed to investigate the impact of in utero BPA exposure on proteins expression related to cardiac function in heart of rat foetuses (Rattus norvegicus). In here, pregnant rats were divided into tween-80 (vehicle control), 0.05 mg/mL and 0.2 mg/mL BPA via drinking water for 19 days: from pregnancy day 2 till 21. Caesarean section was conducted on pregnancy day 21 to collect plasma and heart of both mother and foetuses. BPA-exposed pregnant rats showed significant increase in blood pressure (BP) and reduction in glycogen content (p<0.05) in comparison to control pregnant rats. Remarkably, reduced expression of cardiac troponin I (cTnI) and redistribution of alpha fetoprotein (AFP) expression were in foetus of BPA-exposed mother in comparison with foetus of control mother. Hypoxia induced factor-1 alpha (HIF-1α) expression was elevated in BPA-exposed foetal heart compared to the control. The findings in here suggest the risk of in utero BPA exposure on both foetus and mother, which may increase the risk of CVD in later life by altering the expression of protein crucial for heart development and function.

 

Keywords: Bisphenol A; cardiovascular disease; foetus; prenatal; protein expression

 

Abstrak

Pendedahan bisfenol A (BPA) in utero dilaporkan mampu meningkatkan risiko penyakit kardiovaskular (CVD). Penyelidikan ini dijalankan untuk mengkaji impak in utero apabila didedah kepada BPA dari segi ekspresi protein berkait dengan fungsi kardiak dalam jantung fetus tikus (Rattus norvegicus). Tikus bunting telah diberi tween-80 (kawalan pembawa), 0.05 mg/mL atau 0.2 mg/mL BPA melalui air minuman selama 19 hari; dari hari kedua bunting hingga hari ke 21. Pembedahan Caesarean dijalankan pada hari bunting ke-21 untuk memperoleh plasma dan jantung daripada fetus dan ibu tikus. Tikus bunting terdedah kepada BPA menunjukkan kenaikan tekanan darah dan penurunan kandungan glikogen yang ketara (p<0.05) berbanding kumpulan kawalan tikus bunting. Didapati ekspresi aruhan hipoksia faktor-1 alfa (HIF-1α) meningkat dalam jantung fetus yang terdedah dengan BPA berbanding kumpulan kawalan. Hasil kajian menunjukkan risiko pendedahan BPA dalam rahim ke atas fetus dan ibu tikus boleh meningkatkan risiko penyakit jantung terutamanya dengan peningkatan umur melalui peningkatan ekspresi protein yang penting dalam perkembangan dan fungsi jantung.

 

Kata kunci: Bisfenol A; ekspresi protein; fetus; penyakit kardiovaskular; pranatal

 

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