- Home<\/a><\/li>
- Introduction<\/a><\/li>
- Schedule<\/a><\/li>
- Registration<\/a><\/li>
- Venue<\/a><\/li>
- Accommodation<\/a><\/li>
- Contact<\/a><\/li><\/ul><\/div>
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INTRODUCTION<\/strong><\/p>\n
The workshop is designed for users who are new to real time quantitative PCR (qPCR), but all users are welcome. User will experience converting RNA to cDNA, optimizing a qPCR reaction, assessing primers efficiency and determining expression level of their genes of interest. Clients will gain experience using DNA binding dyes chemistries. Lectures will include an overview of gene expression, assay design and optimization, template preparation, analysis of results, additional applications of real-time PCR technology, and many more. Data analysis and simple statistical test will be included. Some previous experience using standard PCR or real time PCR is helpful but not necessary for workshop attendees.<\/p>\n
OBJECTIVES<\/strong><\/p>\n
Our objectives are:<\/p>\n
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- To give participants a combination of lecture and hands-on laboratory experience using qPCR methods<\/li>\n
- To expose and introduce a basic concept of qPCR and the analysis involved<\/li>\n
- To provide the fundamentals of cDNA synthesis, quality controls in qPCR<\/li>\n
- To promote awareness of the applications of qPCR in research<\/li>\n
- To support research in Molecular Medicine, especially for the local scientists<\/li>\n<\/ul>\n
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\n\t Time\t<\/strong>\t<\/th> Day 1<\/strong>\t<\/th> Day 2 <\/strong>\t<\/th>\n<\/tr>\n<\/thead>\n\n \n\t 8:00 am<\/td> Registration and breakfast<\/td> Breakfast<\/td>\n<\/tr>\n \n\t 9:00 am<\/td> Lecture 2:
\nIntroduction to fundamentals of qPCR
\n(Seminar room, Level 5)
\n<\/td>Hands-on 3:
\nqPCR standard curve analysis
\n(MBU, Level 4)
\n<\/td>\n<\/tr>\n\n\t 10:30 am<\/td> Hands-on 1:
\ncDNA synthesis
\n(MBU, Level 4)
\n<\/td>Hands-on 4:
\nPerforming relative quantification qPCR on Biorad CFX96
\n(MBU, Level 4)
\n<\/td>\n<\/tr>\n\n\t 12:30 - 1:00 pm<\/td> UMBI lab visits and photo sessions<\/td>\n<\/tr>\n \n\t 1:00 pm<\/td> Lunch
\n(Level 1)
\n<\/td>Lunch
\n(Level 1)
\n<\/td>\n<\/tr>\n\n\t 2:30 pm<\/td> Hands-on 2:
\nqPCR primers optimization (gradient qPCR)
\n(MBU, Level 4)
\n<\/td>Hands-on 5:
\nData analysis of Relative quantification qPCR
\n(Bioinformatics lab, Level 3)
\n<\/td>\n<\/tr>\n\n\t 3:30 pm<\/td> Lecture 2:
\nApplications of qPCR in research - UMBI\u2019s experience
\n(Bioinformatics lab, Level 3)
\n<\/td>\n<\/tr>\n\n\t 4:00 pm<\/td> Day 1 Review and Tea<\/td> Day 2 Review and Tea<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n <\/li>
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- Introduction<\/a><\/li>